(Circular) (Six-base) MAPSORT of: pFN21AB7796.seq Check: 7686 from: 1 to: 6259 pFN21AB7796 6259 bp With 182 enzymes: SgfI * December 8, 2011 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3602 1937 Size: 1665 4594 Acc65I G'GTAC_C Cuts at: 2026 3580 2026 Size: 1554 4705 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2657 4322 5706 5974 1632 Size: 806 219 1665 1384 268 1917 Fragments arranged by size: 1917 1665 1384 806 268 219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 5158 6135 1203 Size: 3955 977 1327 Fragments arranged by size: 3955 1327 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6259 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2981 1184 Size: 1797 4462 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2613 2645 2613 Size: 32 6227 AlwNI CAG_nnn'CTG Cuts at: 2983 3026 4002 5574 2983 Size: 43 976 1572 3668 Fragments arranged by size: 3668 1572 976 43 ApaLI G'TGCA_C Cuts at: 2966 4316 5472 2966 Size: 1350 1156 3753 Fragments arranged by size: 3753 1350 1156 ApoI r'AATT_y Cuts at: 1493 3568 3794 3946 6055 1493 Size: 2075 226 152 2109 1697 Fragments arranged by size: 2109 2075 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 3584 1870 Size: 73 1641 4545 Fragments arranged by size: 4545 1641 73 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 3106 3184 3412 3580 Size: 325 217 627 239 1080 78 228 168 Cuts at: 3580 5035 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1080 627 325 239 228 217 168 78 BanII G_rGCy'C Cuts at: 1391 2606 3061 3578 1391 Size: 1215 455 517 4072 Fragments arranged by size: 4072 1215 517 455 BbeI G_GCGC'C Cuts at: 3110 3110 Size: 6259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 928 Size: 971 229 5059 Fragments arranged by size: 5059 971 229 BbvCI CC'TCA_GC Cuts at: 2976 2976 Size: 6259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2020 2054 4450 4484 1903 Size: 34 83 34 2396 34 3678 Fragments arranged by size: 3678 2396 83 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3393 4870 Size: 107 122 71 631 605 337 1384 1477 Cuts at: 4870 136 Size: 1525 Fragments arranged by size: 1525 1477 1384 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 2430 2451 2970 3189 3417 4320 5476 1790 Size: 640 21 519 219 228 903 1156 2573 Fragments arranged by size: 2573 1156 903 640 519 228 219 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 3990 4948 Size: 416 53 83 399 24 1754 819 958 Cuts at: 4948 442 Size: 1753 Fragments arranged by size: 1754 1753 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2705 2714 3058 4919 1239 Size: 1466 9 344 1861 2579 Fragments arranged by size: 2579 1861 1466 344 9 Bpu10I CC'TnA_GC Cuts at: 2976 2976 Size: 6259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4384 5249 5547 4384 Size: 865 298 5096 Fragments arranged by size: 5096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 4922 882 Size: 617 774 2649 2219 Fragments arranged by size: 2649 2219 774 617 BsaAI yAC'GTr Cuts at: 493 1204 4050 493 Size: 711 2846 2702 Fragments arranged by size: 2846 2702 711 BsaBI GATnn'nnATC Cuts at: 3161 6253 3161 Size: 3092 3167 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3107 3422 4066 4448 Size: 53 83 186 936 1574 315 644 382 Cuts at: 4448 5083 275 Size: 635 1451 Fragments arranged by size: 1574 1451 936 644 635 382 315 186 83 53 BsaWI w'CCGG_w Cuts at: 2338 4692 5090 5364 5511 5922 2338 Size: 2354 398 274 147 411 2675 Fragments arranged by size: 2675 2354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2613 2643 3056 Size: 30 165 30 867 30 313 30 413 Cuts at: 3056 3086 1178 Size: 30 4351 Fragments arranged by size: 4351 867 413 313 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2516 2519 2702 2756 3138 1186 Size: 1189 141 3 183 54 382 4307 Fragments arranged by size: 4307 1189 382 183 141 54 3 BseYI C'CCAG_C Cuts at: 2125 2394 5462 5958 2125 Size: 269 3068 496 2426 Fragments arranged by size: 3068 2426 496 269 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 1163 Size: 573 1065 4621 Fragments arranged by size: 4621 1065 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2045 2676 3709 4470 5498 1540 Size: 275 186 44 631 1033 761 1028 2301 Fragments arranged by size: 2301 1033 1028 761 631 275 186 44 BsiHKAI G_wGCw'C Cuts at: 2478 2970 4320 4405 5476 2478 Size: 492 1350 85 1071 3261 Fragments arranged by size: 3261 1350 1071 492 85 BsmI GAATG_Cn' Cuts at: 3770 3863 3770 Size: 93 6166 BsmBI CGTCTCn'nnnn_ Cuts at: 2257 2257 Size: 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2451 2478 2606 2970 3061 3189 Size: 399 640 21 27 128 364 91 128 Cuts at: 3189 3417 3578 4320 4405 5476 1391 Size: 228 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 399 364 228 161 128 128 91 85 27 21 BspEI T'CCGG_A Cuts at: 2338 5922 2338 Size: 3584 2675 BspHI T'CATG_A Cuts at: 3093 4148 5878 3093 Size: 1055 1730 3474 Fragments arranged by size: 3474 1730 1055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2773 3614 844 Size: 1929 841 3489 Fragments arranged by size: 3489 1929 841 BsrBI CCG'CTC Cuts at: 1394 1823 4146 1394 Size: 429 2323 3507 Fragments arranged by size: 3507 2323 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4754 4928 65 Size: 1183 3506 174 1396 Fragments arranged by size: 3506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2589 2882 2970 3347 3371 4903 Size: 110 234 545 293 88 377 24 1532 Cuts at: 4903 5090 1700 Size: 187 2869 Fragments arranged by size: 2869 1532 545 377 293 234 187 110 88 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6259 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 3026 794 Size: 2232 4027 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2839 3544 2329 Size: 510 705 5044 Fragments arranged by size: 5044 705 510 BstYI r'GATC_y Cuts at: 1972 2400 3589 3956 4341 4358 5810 5896 5908 Size: 428 1189 367 385 17 1452 86 12 Cuts at: 5908 6254 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1189 428 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3150 3150 Size: 6259 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 2242 3366 513 Size: 552 939 193 45 1124 3406 Fragments arranged by size: 3406 1124 939 552 193 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5520 Fragments arranged by size: 5520 683 56 BtsI GCAGTG_nn' Cuts at: 3775 4569 4589 3775 Size: 794 20 5445 Fragments arranged by size: 5445 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2083 2118 529 Size: 35 1519 35 4670 Fragments arranged by size: 4670 1519 35 35 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2042 2104 2673 Size: 54 1425 50 275 18 212 62 569 Cuts at: 2673 3243 3369 3385 3394 3706 4595 6224 8 Size: 570 126 16 9 312 889 1629 43 Fragments arranged by size: 1629 1425 889 570 569 312 275 212 126 62 54 50 43 18 16 9 EagI C'GGCC_G Cuts at: 1537 1812 2042 2673 3706 1537 Size: 275 230 631 1033 4090 Fragments arranged by size: 4090 1033 631 275 230 EarI CTCTTCn'nnn_ Cuts at: 1353 4189 1353 Size: 2836 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 4846 5220 5366 387 Size: 1064 138 570 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2657 2705 2714 3058 4322 4919 Size: 393 806 219 48 9 344 1264 597 Cuts at: 4919 5706 5974 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1264 806 787 597 393 344 268 219 48 9 EcoNI CCTnn'n_nnAGG Cuts at: 2707 2707 Size: 6259 EcoO109I rG'GnC_Cy Cuts at: 2712 3456 2712 Size: 744 5515 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 3110 5406 1678 Size: 1432 2296 2531 Fragments arranged by size: 2531 2296 1432 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2588 2600 2620 Size: 32 135 32 698 32 286 12 20 Cuts at: 2620 2632 3029 3061 4900 4932 4974 5006 1373 Size: 12 397 32 1839 32 42 32 2626 Fragments arranged by size: 2626 1839 698 397 286 135 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3603 3849 669 Size: 988 281 1665 246 3079 Fragments arranged by size: 3079 1665 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6259 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KasI G'GCGC_C Cuts at: 3106 3106 Size: 6259 KpnI G_GTAC'C Cuts at: 2030 3584 2030 Size: 1554 4705 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 2639 3138 5373 5557 1305 Size: 1293 41 499 2235 184 2007 Fragments arranged by size: 2235 2007 1293 499 184 41 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 10 Size: 54 1425 343 274 4163 Fragments arranged by size: 4163 1425 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2885 4217 4576 4735 518 Size: 648 1719 1332 359 159 2042 Fragments arranged by size: 2042 1719 1332 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 2230 2394 2683 2839 2911 3023 3223 Size: 519 105 164 289 156 72 112 200 Cuts at: 3223 3264 3274 3675 4352 5500 5745 1606 Size: 41 10 401 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 519 401 289 245 200 164 156 112 105 72 41 10 NaeI GCC'GGC Cuts at: 2046 3373 2046 Size: 1327 4932 NarI GG'CG_CC Cuts at: 3107 3107 Size: 6259 NcoI C'CATG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4575 Fragments arranged by size: 4575 939 552 193 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NgoMIV G'CCGG_C Cuts at: 2044 3371 2044 Size: 1327 4932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 2389 2890 3617 5162 1132 Size: 97 1160 501 727 1545 2229 Fragments arranged by size: 2229 1545 1160 727 501 97 PasI CC'CwG_GG Cuts at: 1348 3417 3453 1348 Size: 2069 36 4154 Fragments arranged by size: 4154 2069 36 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6259 PfoI T'CCnGG_A Cuts at: 2403 2403 Size: 6259 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2613 2645 4328 4360 5841 5873 2613 Size: 32 1683 32 1481 32 2999 Fragments arranged by size: 2999 1683 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2712 2712 Size: 6259 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PstI C_TGCA'G Cuts at: 830 3022 3031 3269 3611 830 Size: 2192 9 238 342 3478 Fragments arranged by size: 3478 2192 342 238 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 2125 2230 2394 2683 2839 2911 3264 2125 Size: 105 164 289 156 72 353 5120 Fragments arranged by size: 5120 353 289 164 156 105 72 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SbfI CC_TGCA'GG Cuts at: 3611 3611 Size: 6259 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 3018 3027 3265 3337 3352 3607 4742 Size: 219 1973 9 238 72 15 255 1135 Cuts at: 4742 5423 5614 826 Size: 681 191 1471 Fragments arranged by size: 1973 1471 1135 681 255 238 219 191 72 15 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3393 3393 Size: 6259 SfoI GGC'GCC Cuts at: 3108 3108 Size: 6259 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 3586 1872 Size: 1714 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4363 5264 5526 820 Size: 197 926 2420 901 262 1553 Fragments arranged by size: 2420 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 2890 3617 1132 Size: 1758 727 3774 Fragments arranged by size: 3774 1758 727 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 2702 3320 1517 Size: 1185 618 4456 Fragments arranged by size: 4456 1185 618 StyI C'CwwG_G Cuts at: 513 1065 2004 2107 2197 3698 513 Size: 552 939 103 90 1501 3074 Fragments arranged by size: 3074 1501 939 552 103 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2451 2893 3346 4286 4303 4456 4641 Size: 1255 871 442 453 940 17 153 185 Cuts at: 4641 5086 325 Size: 445 1498 Fragments arranged by size: 1498 1255 940 871 453 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2364 4505 6217 Size: 274 80 33 51 1443 387 2141 1712 Cuts at: 6217 96 Size: 138 Fragments arranged by size: 2141 1712 1443 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 6219 6251 3056 Size: 32 3131 32 3064 Fragments arranged by size: 3131 3064 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2284 2655 1316 Size: 968 371 4920 Fragments arranged by size: 4920 968 371 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3517 3517 Size: 6259 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6259 XmaI C'CCGG_G Cuts at: 1870 3584 1870 Size: 1714 4545 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI