################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglI BglII BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 20 GTAC GT^AC AlwI 329 GGATC GGATC(4/5) AlwNI 312 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 171 CCTAAGG CC^T[ACGT]AGG AvrII 69 CCTAGG C^CTAGG BbvCI 12 CCTCAGC CCTCAGC(-5/-2) BciVI 287 GTATCC GTATCC(6/5) BfaI 70 CTAG C^TAG BlnI 69 CCTAGG C^CTAGG BlpI 33 GCTCAGC GC^T[ACGT]AGC Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC BsaJI 69 CCTAGG C^C[ACGT][ACGT]GG BsmAI 338 GAGAC GTCTC(1/5) BsmFI 266 GGGAC GGGAC(10/14) BstF5I 153 CATCC GGATG(2/0) Bsu36I 171 CCTAAGG CC^T[ACGT]AGG CelII 33 GCTCAGC GC^T[ACGT]AGC DpnII 330 GATC ^GATC DraI 193 TTTAAA TTT^AAA Eco81I 171 CCTAAGG CC^T[ACGT]AGG EcoT14I 69 CCTAGG C^C[AT][AT]GG FauI 4 CCCGC CCCGC(4/6) FokI 153 CATCC GGATG(9/13) HinfI 142 GATTC G^A[ACGT]TC HphI 281 TCACC GGTGA(8/7) MaeI 70 CTAG C^TAG MboI 330 GATC ^GATC MseI 194 TTAA T^TAA MspA1I 15 CAGCGG C[AC]G^C[GT]G NdeII 330 GATC ^GATC NlaIII 60 CATG CATG^ NspI 59 ACATGC [GA]CATG^[CT] RsaI 20 GTAC GT^AC Sau3AI 330 GATC ^GATC StuI 228 AGGCCT AGG^CCT StyI 69 CCTAGG C^C[AT][AT]GG TfiI 142 GATTC G^A[AT]TC Tru9I 194 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 5 CCGC CCGC(-3/-1) 17 GCGG AcsI 190 AAATTT [GA]^AATT[CT] 208 GAATTT ApoI 190 AAATTT [GA]^AATT[CT] 208 GAATTT AvaII 26 GGACC G^G[AT]CC 320 GGACC BbvI 38 GCAGC GCAGC(8/12) 167 GCAGC BssKI 231 CCTGG ^CC[ACGT]GG 351 CCTGG BstNI 231 CCTGG CC^[AT]GG 351 CCTGG Cac8I 3 GCCCGC GC[ACGT]^[ACGT]GC 63 GCTGGC EcoRII 231 CCTGG ^CC[AT]GG 351 CCTGG Fnu4HI 38 GCAGC GC^[ACGT]GC 167 GCAGC HaeIII 66 GGCC GG^CC 229 GGCC ItaI 38 GCAGC GC^[ACGT]GC 167 GCAGC MvaI 231 CCTGG CC^[AT]GG 351 CCTGG NlaIV 26 GGACCC GG[ACGT]^[ACGT]CC 320 GGACCC ScrFI 231 CCTGG CC^[ACGT]GG 351 CCTGG TaqI 50 TCGA T^CGA 332 TCGA TseI 38 GCAGC G^C[AT]GC 167 GCAGC Tsp509I 191 AATT ^AATT 209 AATT TthHB8I 50 TCGA T^CGA 332 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### Cfr13I DdeI MboII MnlI Sau96I