################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmFI BsmI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI HaeII HaeIII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 7 CGCG CG^CG AccIII 30 TCCGGA T^CCGGA AfaI 42 GTAC GT^AC AlwI 88 GGATC GGATC(4/5) AspHI 14 GAGCTC G[AT]GC[AT]^C AvaI 19 CCCGGG C^[CT]CG[GA]G AvaII 57 GGTCC G^G[AT]CC AviII 122 TGCGCA TGC^GCA BanII 14 GAGCTC G[GA]GC[CT]^C BbsI 229 GAAGAC GAAGAC(2/6) BcgI 180 CGACCAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 153 GCTCAGC GC^T[ACGT]AGC BmyI 14 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 153 GCTCAGC GC^T[ACGT]AGC BpuAI 229 GAAGAC GAAGAC(2/6) BseAI 30 TCCGGA T^CCGGA BsgI 188 GTGCAG GTGCAG(16/14) BsiHKAI 14 GAGCTC G[AT]GC[AT]^C BsiYI 128 CCTATGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 128 CCTATGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 223 GAGAC GTCTC(1/5) BsmBI 223 GAGACG CGTCTC(1/5) BsoBI 19 CCCGGG C^[CT]CG[GA]G Bsp1286I 14 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 41 TGTACA T^GTACA BspEI 30 TCCGGA T^CCGGA BsrGI 41 TGTACA T^GTACA BstUI 7 CGCG CG^CG CelII 153 GCTCAGC GC^T[ACGT]AGC CfoI 123 GCGC GCG^C Cfr13I 57 GGTCC G^G[ACGT]CC DdeI 154 CTCAG C^T[ACGT]AG DpnII 89 GATC ^GATC EarI 24 GAAGAG CTCTTC(1/4) FspI 122 TGCGCA TGC^GCA HhaI 123 GCGC GCG^C HinP1I 123 GCGC G^CGC HincII 142 GTCAAC GT[CT]^[GA]AC HindII 142 GTCAAC GT[CT]^[GA]AC HinfI 27 GAGTC G^A[ACGT]TC HphI 201 GGTGA GGTGA(8/7) Ksp632I 24 GAAGAG CTCTTC(1/4) MaeII 197 ACGT A^CGT MboI 89 GATC ^GATC MroI 30 TCCGGA T^CCGGA MspA1I 160 CAGCTG C[AC]G^C[GT]G MvnI 7 CGCG CG^CG NdeII 89 GATC ^GATC NlaIII 72 CATG CATG^ PleI 27 GAGTC GAGTC(4/5) PvuII 160 CAGCTG CAG^CTG RsaI 42 GTAC GT^AC SacI 14 GAGCTC GAGCT^C Sau3AI 89 GATC ^GATC Sau96I 57 GGTCC G^G[ACGT]CC SmaI 19 CCCGGG CCC^GGG SspBI 41 TGTACA T^GTACA TaqI 179 TCGA T^CGA TthHB8I 179 TCGA T^CGA XmaCI 19 CCCGGG C^CCGGG XmaI 19 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BcnI 19 CCCGG CC^[GC]GG 20 CCGGG BsaJI 19 CCCGGG C^C[ACGT][ACGT]GG 183 CCAGGG BsaWI 30 TCCGGA [AT]^CCGG[AT] 233 ACCGGA BstNI 183 CCAGG CC^[AT]GG 215 CCAGG EcoRII 183 CCAGG ^CC[AT]GG 215 CCAGG MvaI 183 CCAGG CC^[AT]GG 215 CCAGG NciI 19 CCCGG CC^[GC]GG 20 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI AluI BbvI BseRI BssKI Cac8I Fnu4HI HapII HpaII ItaI MboII MnlI MspI MwoI ScrFI TseI