(Circular) (Six-base) MAPSORT of: pFN21AE1895.seq Check: 9914 from: 1 to: 5956 pFN21AE1895 5956 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2219 2878 2219 Size: 659 5297 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 4019 5403 5671 1632 Size: 563 1824 1384 268 1917 Fragments arranged by size: 1917 1824 1384 563 268 AfeI AGC'GCT Cuts at: 2573 3146 2573 Size: 573 5383 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3257 1184 Size: 2073 3883 AlwNI CAG_nnn'CTG Cuts at: 2154 3699 5271 2154 Size: 1545 1572 2839 Fragments arranged by size: 2839 1572 1545 ApaI G_GGCC'C Cuts at: 2071 2077 2443 3002 2071 Size: 6 366 559 5025 Fragments arranged by size: 5025 559 366 6 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2861 3281 1870 Size: 73 127 791 420 4545 Fragments arranged by size: 4545 791 420 127 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3150 3277 4732 618 Size: 325 217 627 1363 127 1455 1842 Fragments arranged by size: 1842 1455 1363 627 325 217 127 BanII G_rGCy'C Cuts at: 1391 2071 2077 2196 2443 2926 3002 3275 1391 Size: 680 6 119 247 483 76 273 4072 Fragments arranged by size: 4072 680 483 273 247 119 76 6 BbeI G_GCGC'C Cuts at: 3154 3154 Size: 5956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3231 928 Size: 971 1332 3653 Fragments arranged by size: 3653 1332 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2505 4567 136 Size: 107 122 71 631 605 833 2062 1525 Fragments arranged by size: 2062 1525 833 631 605 122 107 71 BglII A'GATC_T Cuts at: 2814 5951 2814 Size: 3137 2819 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2071 2077 2169 2275 2443 3002 4017 5173 Size: 281 6 92 106 168 559 1015 1156 Cuts at: 5173 1790 Size: 2573 Fragments arranged by size: 2573 1156 1015 559 281 168 106 92 6 BmgBI CAC'GTC Cuts at: 1318 3016 1318 Size: 1698 4258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2614 3215 4616 1239 Size: 1097 278 601 1401 2579 Fragments arranged by size: 2579 1401 1097 601 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3125 4081 4946 5244 3125 Size: 956 865 298 3837 Fragments arranged by size: 3837 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2858 3114 4619 882 Size: 617 1359 256 1505 2219 Fragments arranged by size: 2219 1505 1359 617 256 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3151 3763 4145 4780 Size: 53 83 186 936 1618 612 382 635 Cuts at: 4780 275 Size: 1451 Fragments arranged by size: 1618 1451 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 1186 Size: 853 5103 BseYI C'CCAG_C Cuts at: 2062 2158 2720 2915 2965 3031 3064 5159 5655 Size: 96 562 195 50 66 33 2095 496 Cuts at: 5655 2062 Size: 2363 Fragments arranged by size: 2363 2095 562 496 195 96 66 50 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 2803 1163 Size: 573 693 374 4316 Fragments arranged by size: 4316 693 573 374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 3112 3406 4167 5195 1540 Size: 275 186 17 1094 294 761 1028 2301 Fragments arranged by size: 2301 1094 1028 761 294 275 186 17 BsiHKAI G_wGCw'C Cuts at: 2552 2622 2926 4017 4102 5173 2552 Size: 70 304 1091 85 1071 3335 Fragments arranged by size: 3335 1091 1071 304 85 70 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2077 2169 2196 2275 2443 2552 Size: 399 281 6 92 27 79 168 109 Cuts at: 2552 2622 2926 3002 3275 4017 4102 5173 1391 Size: 70 304 76 273 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 281 273 168 109 92 85 79 76 70 27 6 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 2504 2878 3311 844 Size: 1375 285 374 433 3489 Fragments arranged by size: 3489 1375 433 374 285 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2746 2969 4600 4787 1700 Size: 110 301 635 223 1631 187 2869 Fragments arranged by size: 2869 1631 635 301 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2545 4016 5028 2545 Size: 1471 1012 3473 Fragments arranged by size: 3473 1471 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2154 794 Size: 1360 4596 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2814 3286 3653 4038 4055 5507 5593 5605 Size: 842 472 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 842 472 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2018 3093 3165 513 Size: 552 939 14 1075 72 3304 Fragments arranged by size: 3304 1075 939 552 72 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2538 487 Size: 683 56 1312 3905 Fragments arranged by size: 3905 1312 683 56 BtsI GCAGTG_nn' Cuts at: 2337 2680 2812 3472 4266 4286 2337 Size: 343 132 660 794 20 4007 Fragments arranged by size: 4007 794 660 343 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2197 2232 529 Size: 35 1633 35 4253 Fragments arranged by size: 4253 1633 35 35 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2334 2748 Size: 54 1425 50 275 18 185 319 414 Cuts at: 2748 3109 3403 4292 5921 8 Size: 361 294 889 1629 43 Fragments arranged by size: 1629 1425 889 414 361 319 294 275 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2015 3109 3403 1537 Size: 275 203 1094 294 4090 Fragments arranged by size: 4090 1094 294 275 203 EarI CTCTTCn'nnn_ Cuts at: 1353 3886 1353 Size: 2533 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2924 2924 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2336 2614 3215 4019 4616 5403 Size: 393 563 141 278 601 804 597 787 Cuts at: 5403 5671 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 601 597 563 393 278 268 141 EcoO109I rG'GnC_Cy Cuts at: 2067 2179 2439 2440 2493 2067 Size: 112 260 1 53 5530 Fragments arranged by size: 5530 260 112 53 1 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 3059 3233 4462 3059 Size: 174 1229 4553 Fragments arranged by size: 4553 1229 174 FspAI rTGC'GCAy Cuts at: 3059 3059 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2048 2315 2575 2778 2879 3043 3148 3154 Size: 370 267 260 203 101 164 105 6 Cuts at: 3154 5103 1678 Size: 1949 2531 Fragments arranged by size: 2531 1949 370 267 260 203 164 105 101 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2716 2748 4597 4629 4671 Size: 32 135 32 1144 32 1849 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1849 1144 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 3150 3150 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 3914 4273 4432 518 Size: 648 1357 1391 359 159 2042 Fragments arranged by size: 2042 1391 1357 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2020 2162 2277 2346 2582 2844 2936 Size: 408 6 142 115 69 236 262 92 Cuts at: 2936 3167 3372 4049 5197 5442 1606 Size: 231 205 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 408 262 245 236 231 205 142 115 92 69 6 NaeI GCC'GGC Cuts at: 2971 2971 Size: 5956 NarI GG'CG_CC Cuts at: 3151 3151 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2969 2969 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 2015 3403 2015 Size: 1388 4568 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 2077 2554 1348 Size: 729 477 4750 Fragments arranged by size: 4750 729 477 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 1108 Size: 1725 4231 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2493 2493 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2067 2073 2439 2998 2067 Size: 6 366 559 5025 Fragments arranged by size: 5025 559 366 6 PstI C_TGCA'G Cuts at: 830 2713 2994 3246 3308 830 Size: 1883 281 252 62 3478 Fragments arranged by size: 3478 1883 281 252 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2844 2844 Size: 5956 SacI G_AGCT'C Cuts at: 2926 2926 Size: 5956 SacII CC_GC'GG Cuts at: 2021 3168 2021 Size: 1147 4809 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2709 2990 3242 3304 4439 5120 5311 Size: 219 1664 281 252 62 1135 681 191 Cuts at: 5311 826 Size: 1471 Fragments arranged by size: 1664 1471 1135 681 281 252 219 191 62 SfoI GGC'GCC Cuts at: 3152 3152 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 2072 3283 1872 Size: 200 1211 4545 Fragments arranged by size: 4545 1211 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 3140 4060 4961 5223 820 Size: 197 926 1197 920 901 262 1553 Fragments arranged by size: 1553 1197 926 920 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SrfI GCCC'GGGC Cuts at: 2072 2072 Size: 5956 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2285 2300 1517 Size: 768 15 5173 Fragments arranged by size: 5173 768 15 StyI C'CwwG_G Cuts at: 513 1065 2004 2766 3395 513 Size: 552 939 762 629 3074 Fragments arranged by size: 3074 939 762 629 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 3983 4000 4153 4338 4783 325 Size: 1255 1188 1215 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1215 1188 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 2896 3026 1316 Size: 1580 130 4246 Fragments arranged by size: 4246 1580 130 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2129 2551 3039 2129 Size: 422 488 5046 Fragments arranged by size: 5046 488 422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 2070 3281 1870 Size: 200 1211 4545 Fragments arranged by size: 4545 1211 200 XmnI GAAnn'nnTTC Cuts at: 3050 4085 3050 Size: 1035 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SwaI