(Linear) (Six-base) MAPSORT of: KIEE1895.seq Check: 8243 from: 1 to: 1251 KIEE1895.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 214 873 1251 Size: 214 659 378 Fragments arranged by size: 659 378 214 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 1251 Size: 190 1061 AfeI AGC'GCT Cuts at: 0 568 1141 1251 Size: 568 573 110 Fragments arranged by size: 573 568 110 AlwNI CAG_nnn'CTG Cuts at: 0 149 1251 Size: 149 1102 ApaI G_GGCC'C Cuts at: 0 66 72 438 997 1251 Size: 66 6 366 559 254 Fragments arranged by size: 559 366 254 66 6 AvaI C'yCGr_G Cuts at: 0 65 856 1251 Size: 65 791 395 Fragments arranged by size: 791 395 65 BanI G'GyrC_C Cuts at: 0 1145 1251 Size: 1145 106 BanII G_rGCy'C Cuts at: 0 66 72 191 438 921 997 1251 Size: 66 6 119 247 483 76 254 Fragments arranged by size: 483 254 247 119 76 66 6 BbeI G_GCGC'C Cuts at: 0 1149 1251 Size: 1149 102 BbsI GAAGACnn'nnnn_ Cuts at: 0 1226 1251 Size: 1226 25 BglI GCCn_nnn'nGGC Cuts at: 0 500 1251 Size: 500 751 BglII A'GATC_T Cuts at: 0 809 1251 Size: 809 442 Bme1580I G_kGCm'C Cuts at: 0 66 72 164 270 438 997 1251 Size: 66 6 92 106 168 559 254 Fragments arranged by size: 559 254 168 106 92 66 6 BmgBI CAC'GTC Cuts at: 0 1011 1251 Size: 1011 240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 312 1251 Size: 312 939 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 609 1210 1251 Size: 331 278 601 41 Fragments arranged by size: 601 331 278 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1120 1251 Size: 1120 131 BsaI GGTCTCn'nnnn_ Cuts at: 0 853 1109 1251 Size: 853 256 142 Fragments arranged by size: 853 256 142 BsaHI Gr'CG_yC Cuts at: 0 1146 1251 Size: 1146 105 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 1251 Size: 34 1217 BseYI C'CCAG_C Cuts at: 0 57 153 715 910 960 1026 1059 1251 Size: 57 96 562 195 50 66 33 192 Fragments arranged by size: 562 195 192 96 66 57 50 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 798 1251 Size: 424 374 453 Fragments arranged by size: 453 424 374 BsiEI CG_ry'CG Cuts at: 0 13 1107 1251 Size: 13 1094 144 Fragments arranged by size: 1094 144 13 BsiHKAI G_wGCw'C Cuts at: 0 547 617 921 1251 Size: 547 70 304 330 Fragments arranged by size: 547 330 304 70 Bsp1286I G_dGCh'C Cuts at: 0 66 72 164 191 270 438 547 617 Size: 66 6 92 27 79 168 109 70 Cuts at: 617 921 997 1251 Size: 304 76 254 Fragments arranged by size: 304 254 168 109 92 79 76 70 66 27 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 214 499 873 1251 Size: 214 285 374 378 Fragments arranged by size: 378 374 285 214 BsrFI r'CCGG_y Cuts at: 0 106 741 964 1251 Size: 106 635 223 287 Fragments arranged by size: 635 287 223 106 BssSI C'ACGA_G Cuts at: 0 540 1251 Size: 540 711 BstAPI GCAn_nnn'nTGC Cuts at: 0 149 1251 Size: 149 1102 BstYI r'GATC_y Cuts at: 0 809 1251 Size: 809 442 BtgI C'CryG_G Cuts at: 0 13 1088 1160 1251 Size: 13 1075 72 91 Fragments arranged by size: 1075 91 72 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 533 1251 Size: 533 718 BtsI GCAGTG_nn' Cuts at: 0 332 675 807 1251 Size: 332 343 132 444 Fragments arranged by size: 444 343 332 132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 192 227 1251 Size: 192 35 1024 Fragments arranged by size: 1024 192 35 EaeI y'GGCC_r Cuts at: 0 10 329 743 1104 1251 Size: 10 319 414 361 147 Fragments arranged by size: 414 361 319 147 10 EagI C'GGCC_G Cuts at: 0 10 1104 1251 Size: 10 1094 147 Fragments arranged by size: 1094 147 10 EcoICRI GAG'CTC Cuts at: 0 919 1251 Size: 919 332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 331 609 1210 1251 Size: 190 141 278 601 41 Fragments arranged by size: 601 278 190 141 41 EcoO109I rG'GnC_Cy Cuts at: 0 62 174 434 435 488 1251 Size: 62 112 260 1 53 763 Fragments arranged by size: 763 260 112 62 53 1 FspI TGC'GCA Cuts at: 0 1054 1228 1251 Size: 1054 174 23 Fragments arranged by size: 1054 174 23 FspAI rTGC'GCAy Cuts at: 0 1054 1251 Size: 1054 197 HaeII r_GCGC'y Cuts at: 0 43 310 570 773 874 1038 1143 1149 Size: 43 267 260 203 101 164 105 6 Cuts at: 1149 1251 Size: 102 Fragments arranged by size: 267 260 203 164 105 102 101 43 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 711 743 1251 Size: 711 32 508 Fragments arranged by size: 711 508 32 KasI G'GCGC_C Cuts at: 0 1145 1251 Size: 1145 106 MslI CAynn'nnrTG Cuts at: 0 518 1251 Size: 518 733 MspA1I CmG'CkG Cuts at: 0 9 15 157 272 341 577 839 931 Size: 9 6 142 115 69 236 262 92 Cuts at: 931 1162 1251 Size: 231 89 Fragments arranged by size: 262 236 231 142 115 92 89 69 9 6 NaeI GCC'GGC Cuts at: 0 966 1251 Size: 966 285 NarI GG'CG_CC Cuts at: 0 1146 1251 Size: 1146 105 NgoMIV G'CCGG_C Cuts at: 0 964 1251 Size: 964 287 NotI GC'GGCC_GC Cuts at: 0 10 1251 Size: 10 1241 PasI CC'CwG_GG Cuts at: 0 72 549 1251 Size: 72 477 702 Fragments arranged by size: 702 477 72 PflMI CCAn_nnn'nTGG Cuts at: 0 828 1251 Size: 828 423 PpuMI rG'GwC_Cy Cuts at: 0 488 1251 Size: 488 763 PspOMI G'GGCC_C Cuts at: 0 62 68 434 993 1251 Size: 62 6 366 559 258 Fragments arranged by size: 559 366 258 62 6 PstI C_TGCA'G Cuts at: 0 708 989 1241 1251 Size: 708 281 252 10 Fragments arranged by size: 708 281 252 10 PvuII CAG'CTG Cuts at: 0 839 1251 Size: 839 412 SacI G_AGCT'C Cuts at: 0 921 1251 Size: 921 330 SacII CC_GC'GG Cuts at: 0 16 1163 1251 Size: 16 1147 88 Fragments arranged by size: 1147 88 16 SfcI C'TryA_G Cuts at: 0 704 985 1237 1251 Size: 704 281 252 14 Fragments arranged by size: 704 281 252 14 SfoI GGC'GCC Cuts at: 0 1147 1251 Size: 1147 104 SmaI CCC'GGG Cuts at: 0 67 1251 Size: 67 1184 SmlI C'TyrA_G Cuts at: 0 1135 1251 Size: 1135 116 SrfI GCCC'GGGC Cuts at: 0 67 1251 Size: 67 1184 StuI AGG'CCT Cuts at: 0 280 295 1251 Size: 280 15 956 Fragments arranged by size: 956 280 15 StyI C'CwwG_G Cuts at: 0 761 1251 Size: 761 490 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 1251 Size: 763 488 Tth111I GACn'n_nGTC Cuts at: 0 891 1021 1251 Size: 891 130 230 Fragments arranged by size: 891 230 130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 124 546 1034 1251 Size: 124 422 488 217 Fragments arranged by size: 488 422 217 124 XmaI C'CCGG_G Cuts at: 0 65 1251 Size: 65 1186 XmnI GAAnn'nnTTC Cuts at: 0 1045 1251 Size: 1045 206 Enzymes that do cut: AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PpuMI PspOMI PstI PvuII SacI SacII SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI TstI XbaI XhoI ZraI