Restriction map for
pFN21AE3105
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AccI AccIII AciI AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbsI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MaeI MaeIII MamI
MboII MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 399 GACGTC GACGT^C
AccII 768 CGCG CG^CG
AcsI 208 GAATTT [GA]^AATT[CT]
AfaI 750 GTAC GT^AC
AflIII 308 ACACGT A^C[GA][CT]GT
AlwNI 43 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 375 GGGCCC GGGCC^C
ApoI 208 GAATTT [GA]^AATT[CT]
Asp700I 472 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 124 CTCGAG C^[CT]CG[GA]G
BbeI 691 GGCGCC GGCGC^C
BbrPI 309 CACGTG CAC^GTG
BbvCI 756 GCTGAGG CCTCAGC(-5/-2)
BclI 239 TGATCA T^GATCA
BpmI 655 CTCCAG CTGGAG(16/14)
BsgI 430 GTGCAG GTGCAG(16/14)
BsmFI 637 GTCCC GGGAC(10/14)
BsoBI 124 CTCGAG C^[CT]CG[GA]G
BsrFI 668 ACCGGC [GA]^CCGG[CT]
BstAPI 727 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 114 TTCGAA TT^CGAA
BstF5I 339 CATCC GGATG(2/0)
BstUI 768 CGCG CG^CG
Cfr10I 668 ACCGGC [GA]^CCGG[CT]
DpnII 240 GATC ^GATC
EcoNI 163 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 239 TGATCA T^GATCA
FokI 339 CATCC GGATG(9/13)
HaeII 691 GGCGCC [GA]GCGC^[CT]
HgaI 582 GACGC GACGC(5/10)
KasI 691 GGCGCC G^GCGCC
MboI 240 GATC ^GATC
MspA1I 607 CAGCTG C[AC]G^C[GT]G
MvnI 768 CGCG CG^CG
NarI 691 GGCGCC GG^CGCC
NdeII 240 GATC ^GATC
NspI 621 GCATGC [GA]CATG^[CT]
NspV 114 TTCGAA TT^CGAA
PaeR7I 124 CTCGAG C^TCGAG
PmaCI 309 CACGTG CAC^GTG
PmlI 309 CACGTG CAC^GTG
PvuII 607 CAGCTG CAG^CTG
RsaI 750 GTAC GT^AC
Sau3AI 240 GATC ^GATC
SfaNI 764 GCATC GCATC(5/9)
SfoI 691 GGCGCC GGC^GCC
SfuI 114 TTCGAA TT^CGAA
SmlI 124 CTCGAG C^T[CT][GA]AG
SphI 621 GCATGC GCATG^C
SspI 118 AATATT AAT^ATT
TfiI 603 GATTC G^A[AT]TC
TspRI 428 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 124 CTCGAG C^TCGAG
XmnI 472 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 399 GACGTC G[GA]^CG[CT]C
691 GGCGCC
AluI 358 AGCT AG^CT
608 AGCT
BsaAI 309 CACGTG [CT]AC^GT[GA]
751 TACGTG
BsaHI 399 GACGTC G[GA]^CG[CT]C
691 GGCGCC
BseRI 493 GAGGAG GAGGAG(10/8)
759 GAGGAG
BsmBI 397 GAGACG CGTCTC(1/5)
580 GAGACG
Cac8I 621 GCATGC GC[ACGT]^[ACGT]GC
660 GCAGGC
DraII 374 AGGGCCC [GA]G^G[ACGT]CC[CT]
375 GGGCCCC
DsaI 32 CCACGG C^C[GA][CT]GG
675 CCACGG
EcoO109I 374 AGGGCCC [GA]G^G[ACGT]CC[CT]
375 GGGCCCC
Hin1I 399 GACGTC G[GA]^CG[CT]C
691 GGCGCC
PleI 546 GACTC GAGTC(4/5)
680 GACTC
TaqI 115 TCGA T^CGA
125 TCGA
TthHB8I 115 TCGA T^CGA
125 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MnlI MseI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI Tru9I TseI Tsp509I