(Linear) (Six-base) MAPSORT of: KIEE6794.seq Check: 924 from: 1 to: 3396 KIEE6794.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AccI GT'mk_AC Cuts at: 0 157 254 2781 2975 3396 Size: 157 97 2527 194 421 Fragments arranged by size: 2527 421 194 157 97 AclI AA'CG_TT Cuts at: 0 849 3396 Size: 849 2547 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3055 3329 3396 Size: 3055 274 67 Fragments arranged by size: 3055 274 67 AhdI GACnn_n'nnGTC Cuts at: 0 3346 3396 Size: 3346 50 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2200 2382 2872 3396 Size: 2200 182 490 524 Fragments arranged by size: 2200 524 490 182 AlwNI CAG_nnn'CTG Cuts at: 0 31 2712 3396 Size: 31 2681 684 Fragments arranged by size: 2681 684 31 ApaI G_GGCC'C Cuts at: 0 2872 3396 Size: 2872 524 ApoI r'AATT_y Cuts at: 0 168 213 667 1108 1267 1929 2289 2418 Size: 168 45 454 441 159 662 360 129 Cuts at: 2418 2840 3396 Size: 422 556 Fragments arranged by size: 662 556 454 441 422 360 168 159 129 45 AseI AT'TA_AT Cuts at: 0 872 1163 2254 3396 Size: 872 291 1091 1142 Fragments arranged by size: 1142 1091 872 291 BanI G'GyrC_C Cuts at: 0 2379 2458 3396 Size: 2379 79 938 Fragments arranged by size: 2379 938 79 BanII G_rGCy'C Cuts at: 0 1120 1176 2099 2872 3396 Size: 1120 56 923 773 524 Fragments arranged by size: 1120 923 773 524 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 1669 1961 3185 3396 Size: 1669 292 1224 211 Fragments arranged by size: 1669 1224 292 211 BciVI GTATCCnnnnn_n' Cuts at: 0 3042 3210 3396 Size: 3042 168 186 Fragments arranged by size: 3042 186 168 Bme1580I G_kGCm'C Cuts at: 0 2872 3396 Size: 2872 524 BmgBI CAC'GTC Cuts at: 0 3065 3396 Size: 3065 331 BmrI ACTGGGnnnn_n' Cuts at: 0 265 2039 3396 Size: 265 1774 1357 Fragments arranged by size: 1774 1357 265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 8 62 1805 3396 Size: 8 54 1743 1591 Fragments arranged by size: 1743 1591 54 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 1298 1652 3136 3396 Size: 724 574 354 1484 260 Fragments arranged by size: 1484 724 574 354 260 BsaI GGTCTCn'nnnn_ Cuts at: 0 1000 1975 3396 Size: 1000 975 1421 Fragments arranged by size: 1421 1000 975 BsaAI yAC'GTr Cuts at: 0 580 1399 3396 Size: 580 819 1997 Fragments arranged by size: 1997 819 580 BsaBI GATnn'nnATC Cuts at: 0 1376 1784 3396 Size: 1376 408 1612 Fragments arranged by size: 1612 1376 408 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 776 806 1163 1193 3396 Size: 776 30 357 30 2203 Fragments arranged by size: 2203 776 357 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1476 3396 Size: 1476 1920 BsiHKAI G_wGCw'C Cuts at: 0 2027 2099 2366 3396 Size: 2027 72 267 1030 Fragments arranged by size: 2027 1030 267 72 BsmI GAATG_Cn' Cuts at: 0 1282 3396 Size: 1282 2114 Bsp1286I G_dGCh'C Cuts at: 0 1120 1176 2027 2099 2366 2872 3396 Size: 1120 56 851 72 267 506 524 Fragments arranged by size: 1120 851 524 506 267 72 56 BspHI T'CATG_A Cuts at: 0 1734 3396 Size: 1734 1662 BsrDI GCAATG_nn' Cuts at: 0 113 587 1781 2856 3396 Size: 113 474 1194 1075 540 Fragments arranged by size: 1194 1075 540 474 113 BsrGI T'GTAC_A Cuts at: 0 240 598 2628 2738 3396 Size: 240 358 2030 110 658 Fragments arranged by size: 2030 658 358 240 110 BstXI CCAn_nnnn'nTGG Cuts at: 0 723 3210 3396 Size: 723 2487 186 Fragments arranged by size: 2487 723 186 BstYI r'GATC_y Cuts at: 0 2820 3396 Size: 2820 576 BstZ17I GTA'TAC Cuts at: 0 158 2976 3396 Size: 158 2818 420 Fragments arranged by size: 2818 420 158 BtsI GCAGTG_nn' Cuts at: 0 1943 2366 3396 Size: 1943 423 1030 Fragments arranged by size: 1943 1030 423 ClaI AT'CG_AT Cuts at: 0 614 2148 3396 Size: 614 1534 1248 Fragments arranged by size: 1534 1248 614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1932 1967 2657 2692 3396 Size: 1932 35 690 35 704 Fragments arranged by size: 1932 704 690 35 35 DraI TTT'AAA Cuts at: 0 1022 2220 2423 3396 Size: 1022 1198 203 973 Fragments arranged by size: 1198 1022 973 203 DraIII CAC_nnn'GTG Cuts at: 0 1798 3081 3396 Size: 1798 1283 315 Fragments arranged by size: 1798 1283 315 DrdI GACnn_nn'nnGTC Cuts at: 0 364 3396 Size: 364 3032 EaeI y'GGCC_r Cuts at: 0 1290 3396 Size: 1290 2106 EarI CTCTTCn'nnn_ Cuts at: 0 41 1379 1494 3362 3396 Size: 41 1338 115 1868 34 Fragments arranged by size: 1868 1338 115 41 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3324 3396 Size: 3324 72 EcoICRI GAG'CTC Cuts at: 0 2097 3396 Size: 2097 1299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 8 62 1805 3055 3329 3396 Size: 8 54 1743 1250 274 67 Fragments arranged by size: 1743 1250 274 67 54 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 241 3396 Size: 241 3155 EcoO109I rG'GnC_Cy Cuts at: 0 727 2869 3396 Size: 727 2142 527 Fragments arranged by size: 2142 727 527 EcoRI G'AATT_C Cuts at: 0 1267 3396 Size: 1267 2129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3060 3396 Size: 3060 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 535 567 1027 1059 1315 1347 2157 2189 Size: 535 32 460 32 256 32 810 32 Cuts at: 2189 2223 2255 2805 2837 3316 3332 3348 3364 Size: 34 32 550 32 479 16 16 16 Cuts at: 3364 3396 Size: 32 Fragments arranged by size: 810 550 535 479 460 256 34 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 0 369 1182 1748 3396 Size: 369 813 566 1648 Fragments arranged by size: 1648 813 566 369 HindIII A'AGCT_T Cuts at: 0 107 2214 3037 3396 Size: 107 2107 823 359 Fragments arranged by size: 2107 823 359 107 HpaI GTT'AAC Cuts at: 0 1182 1748 3396 Size: 1182 566 1648 Fragments arranged by size: 1648 1182 566 MfeI C'AATT_G Cuts at: 0 555 632 3396 Size: 555 77 2764 Fragments arranged by size: 2764 555 77 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2353 3396 Size: 2353 1043 MscI TGG'CCA Cuts at: 0 1292 3396 Size: 1292 2104 MslI CAynn'nnrTG Cuts at: 0 802 3396 Size: 802 2594 MspA1I CmG'CkG Cuts at: 0 1468 3396 Size: 1468 1928 NspI r_CATG'y Cuts at: 0 2002 2063 3396 Size: 2002 61 1333 Fragments arranged by size: 2002 1333 61 PpuMI rG'GwC_Cy Cuts at: 0 727 3396 Size: 727 2669 PsiI TTA'TAA Cuts at: 0 304 3396 Size: 304 3092 PspOMI G'GGCC_C Cuts at: 0 2868 3396 Size: 2868 528 PstI C_TGCA'G Cuts at: 0 921 1950 1980 2361 3396 Size: 921 1029 30 381 1035 Fragments arranged by size: 1035 1029 921 381 30 PvuII CAG'CTG Cuts at: 0 1468 3396 Size: 1468 1928 SacI G_AGCT'C Cuts at: 0 2099 3396 Size: 2099 1297 SapI GCTCTTCn'nnn_ Cuts at: 0 1379 3396 Size: 1379 2017 ScaI AGT'ACT Cuts at: 0 2208 2635 3396 Size: 2208 427 761 Fragments arranged by size: 2208 761 427 SfcI C'TryA_G Cuts at: 0 530 690 917 1421 1457 1946 1976 2357 Size: 530 160 227 504 36 489 30 381 Cuts at: 2357 2775 2860 3309 3396 Size: 418 85 449 87 Fragments arranged by size: 530 504 489 449 418 381 227 160 87 85 36 30 SmlI C'TyrA_G Cuts at: 0 703 1313 1667 3151 3396 Size: 703 610 354 1484 245 Fragments arranged by size: 1484 703 610 354 245 SphI G_CATG'C Cuts at: 0 2002 3396 Size: 2002 1394 SspI AAT'ATT Cuts at: 0 3230 3396 Size: 3230 166 StuI AGG'CCT Cuts at: 0 2009 3396 Size: 2009 1387 StyI C'CwwG_G Cuts at: 0 831 973 3044 3396 Size: 831 142 2071 352 Fragments arranged by size: 2071 831 352 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2010 3396 Size: 2010 1386 TatI w'GTAC_w Cuts at: 0 240 453 598 2206 2353 2628 2633 2738 Size: 240 213 145 1608 147 275 5 105 Cuts at: 2738 3396 Size: 658 Fragments arranged by size: 1608 658 275 240 213 147 145 105 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1782 1814 3396 Size: 1782 32 1582 Fragments arranged by size: 1782 1582 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2689 2862 3396 Size: 2689 173 534 Fragments arranged by size: 2689 534 173 XmnI GAAnn'nnTTC Cuts at: 0 470 1610 3396 Size: 470 1140 1786 Fragments arranged by size: 1786 1140 470 Enzymes that do cut: AccI AclI AcuI AhdI AlfI AlwNI ApaI ApoI AseI BanI BanII BbsI BciVI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NspI PpuMI PsiI PspOMI PstI PvuII SacI SapI ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AleI AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgI BtgZI EagI EcoRV FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI