################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII BalI BbeI BbrPI BbsI BbvCI BbvI BcgI BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FauI Fnu4HI FseI FspI HaeII Hin1I HindIII ItaI KasI KpnI KspI MamI MluNI MroI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SnaBI SphI Sse8387I SspI SwaI TseI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AseI 2094 ATTAAT AT^TAAT AsnI 2094 ATTAAT AT^TAAT AvaI 1919 CCCGGG C^[CT]CG[GA]G AvaII 1534 GGACC G^G[AT]CC AvrII 1806 CCTAGG C^CTAGG BamHI 589 GGATCC G^GATCC BglI 1543 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 14 AGATCT A^GATCT BlnI 1806 CCTAGG C^CTAGG BlpI 144 GCTGAGC GC^T[ACGT]AGC Bpu1102I 144 GCTGAGC GC^T[ACGT]AGC BsaAI 1363 TACGTG [CT]AC^GT[GA] BsaI 394 GGTCTC GGTCTC(1/5) BseRI 1255 GAGGAG GAGGAG(10/8) BsoBI 1919 CCCGGG C^[CT]CG[GA]G Bsp1407I 2217 TGTACA T^GTACA BsrGI 2217 TGTACA T^GTACA BstBI 1622 TTCGAA TT^CGAA CelII 144 GCTGAGC GC^T[ACGT]AGC CfoI 264 GCGC GCG^C DrdI 1325 GACTTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1146 CTCTTC CTCTTC(1/4) EcoNI 1801 CCTGACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 794 GATATC GAT^ATC EcoT22I 384 ATGCAT ATGCA^T HhaI 264 GCGC GCG^C HinP1I 264 GCGC G^CGC HincII 1576 GTTAAC GT[CT]^[GA]AC HindII 1576 GTTAAC GT[CT]^[GA]AC HpaI 1576 GTTAAC GTT^AAC Ksp632I 1146 CTCTTC CTCTTC(1/4) MfeI 1113 CAATTG C^AATTG MluI 786 ACGCGT A^CGCGT MunI 1113 CAATTG C^AATTG NsiI 384 ATGCAT ATGCA^T NspV 1622 TTCGAA TT^CGAA PshBI 2094 ATTAAT AT^TAAT SfuI 1622 TTCGAA TT^CGAA SmaI 1919 CCCGGG CCC^GGG SpeI 48 ACTAGT A^CTAGT SspBI 2217 TGTACA T^GTACA StuI 449 AGGCCT AGG^CCT XbaI 942 TCTAGA T^CTAGA XmaCI 1919 CCCGGG C^CCGGG XmaI 1919 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 265 CGCG CG^CG 787 CGCG BciVI 740 GGATAC GTATCC(6/5) 1360 GGATAC BclI 633 TGATCA T^GATCA 1590 TGATCA BpmI 2021 CTCCAG CTGGAG(16/14) 298 CTGGAG BsmFI 578 GGGAC GGGAC(10/14) 179 GTCCC BspHI 1011 TCATGA T^CATGA 1335 TCATGA BspLU11I 468 ACATGT A^CATGT 1159 ACATGT BstUI 265 CGCG CG^CG 787 CGCG Cac8I 856 GCTCGC GC[ACGT]^[ACGT]GC 1664 GCAGGC DsaI 1544 CCATGG C^C[GA][CT]GG 1790 CCATGG EcoRI 1416 GAATTC G^AATTC 1619 GAATTC FbaI 633 TGATCA T^GATCA 1590 TGATCA HgaI 1097 GACGC GACGC(5/10) 1868 GCGTC MslI 381 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2070 CATCATAATG MvnI 265 CGCG CG^CG 787 CGCG NcoI 1544 CCATGG C^CATGG 1790 CCATGG PstI 196 CTGCAG CTGCA^G 1885 CTGCAG RcaI 1011 TCATGA T^CATGA 1335 TCATGA SexAI 1000 ACCTGGT A^CC[AT]GGT 1302 ACCAGGT TaqI 1623 TCGA T^CGA 1654 TCGA TthHB8I 1623 TCGA T^CGA 1654 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AcsI AfaI AflII AflIII AluI AlwI ApoI BanI BanII BcnI BfaI BfrI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI EcoRII EcoT14I FokI HaeIII HapII HinfI HpaII HphI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I Tsp45I Tsp509I TspRI XcmI XhoII