(Circular) (Six-base) MAPSORT of: pFN21AE6222.seq Check: 4985 from: 1 to: 5395 pFN21AE6222 5395 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3205 278 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2738 1937 Size: 801 4594 Acc65I G'GTAC_C Cuts at: 2716 2716 Size: 5395 AclI AA'CG_TT Cuts at: 1563 2694 3522 3895 1563 Size: 1131 828 373 3063 Fragments arranged by size: 3063 1131 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3458 4842 5110 1632 Size: 1826 1384 268 1917 Fragments arranged by size: 1917 1826 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5198 AflIII A'CryG_T Cuts at: 1203 4294 5271 1203 Size: 3091 977 1327 Fragments arranged by size: 3091 1327 977 AgeI A'CCGG_T Cuts at: 4226 4226 Size: 5395 AhdI GACnn_n'nnGTC Cuts at: 4124 4124 Size: 5395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5395 AlwNI CAG_nnn'CTG Cuts at: 3138 4710 3138 Size: 1572 3823 ApaI G_GGCC'C Cuts at: 2024 2024 Size: 5395 ApaLI G'TGCA_C Cuts at: 3452 4608 3452 Size: 1156 4239 ApoI r'AATT_y Cuts at: 1493 2704 2930 3082 5191 1493 Size: 1211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 226 152 AseI AT'TA_AT Cuts at: 160 1032 3949 160 Size: 872 2917 1606 Fragments arranged by size: 2917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 AvaI C'yCGr_G Cuts at: 1870 1943 2720 1870 Size: 73 777 4545 Fragments arranged by size: 4545 777 73 BamHI G'GATC_C Cuts at: 2725 3092 2725 Size: 367 5028 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 4171 618 Size: 325 217 627 929 1455 1842 Fragments arranged by size: 1842 1455 929 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2714 1391 Size: 633 690 4072 Fragments arranged by size: 4072 690 633 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 2566 928 Size: 971 375 292 3757 Fragments arranged by size: 3757 971 375 292 BbvCI CC'TCA_GC Cuts at: 2043 2043 Size: 5395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3586 3620 1903 Size: 34 1649 34 3678 Fragments arranged by size: 3678 1649 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3287 4497 3287 Size: 1210 4185 BclI T'GATC_A Cuts at: 1548 1548 Size: 5395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4006 136 Size: 107 122 71 631 605 2334 1525 Fragments arranged by size: 2334 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2478 5390 2478 Size: 2912 2483 BlpI GC'TnA_GC Cuts at: 2812 2812 Size: 5395 Bme1580I G_kGCm'C Cuts at: 1790 2024 3456 4612 1790 Size: 234 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3126 4084 442 Size: 416 53 83 399 24 1709 958 1753 Fragments arranged by size: 1753 1709 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2625 4055 1239 Size: 1386 1430 2579 Fragments arranged by size: 2579 1430 1386 Bpu10I CC'TnA_GC Cuts at: 2043 2043 Size: 5395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3520 4385 4683 3520 Size: 865 298 4232 Fragments arranged by size: 4232 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2470 4058 882 Size: 617 971 1588 2219 Fragments arranged by size: 2219 1588 971 617 BsaAI yAC'GTr Cuts at: 493 1204 3186 493 Size: 711 1982 2702 Fragments arranged by size: 2702 1982 711 BsaBI GATnn'nnATC Cuts at: 5389 5389 Size: 5395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3202 3584 4219 275 Size: 53 83 186 936 1669 382 635 1451 Fragments arranged by size: 1669 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3828 4226 4500 4647 5058 3828 Size: 398 274 147 411 4165 Fragments arranged by size: 4165 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2598 2628 1178 Size: 30 165 30 1195 30 3945 Fragments arranged by size: 3945 1195 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5395 BseYI C'CCAG_C Cuts at: 2539 4598 5094 2539 Size: 2059 496 2840 Fragments arranged by size: 2840 2059 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4822 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2845 3606 4634 1540 Size: 275 186 844 761 1028 2301 Fragments arranged by size: 2301 1028 844 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 2514 2654 3456 3541 4612 2496 Size: 18 140 802 85 1071 3279 Fragments arranged by size: 3279 1071 802 140 85 18 BsmI GAATG_Cn' Cuts at: 2587 2906 2999 2587 Size: 319 93 4983 Fragments arranged by size: 4983 319 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2496 2514 2654 2714 3456 3541 Size: 399 234 472 18 140 60 742 85 Cuts at: 3541 4612 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 234 140 85 60 18 BspEI T'CCGG_A Cuts at: 5058 5058 Size: 5395 BspHI T'CATG_A Cuts at: 2577 3284 5014 2577 Size: 707 1730 2958 Fragments arranged by size: 2958 1730 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2750 844 Size: 1436 470 3489 Fragments arranged by size: 3489 1436 470 BsrBI CCG'CTC Cuts at: 1394 1823 3282 1394 Size: 429 1459 3507 Fragments arranged by size: 3507 1459 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3890 4064 65 Size: 1183 2642 174 1396 Fragments arranged by size: 2642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4039 4226 1700 Size: 110 2229 187 2869 Fragments arranged by size: 2869 2229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5395 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5395 BssSI C'ACGA_G Cuts at: 3455 4467 3455 Size: 1012 4383 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5395 BstBI TT'CG_AA Cuts at: 4199 4199 Size: 5395 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5395 BstXI CCAn_nnnn'nTGG Cuts at: 2483 2483 Size: 5395 BstYI r'GATC_y Cuts at: 1972 2478 2725 3092 3477 3494 4946 5032 5044 Size: 506 247 367 385 17 1452 86 12 Cuts at: 5044 5390 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 506 385 367 346 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4843 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4656 Fragments arranged by size: 4656 683 56 BtsI GCAGTG_nn' Cuts at: 2220 2911 3705 3725 2220 Size: 691 794 20 3890 Fragments arranged by size: 3890 794 691 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5360 DraI TTT'AAA Cuts at: 2699 2699 Size: 5395 DraIII CAC_nnn'GTG Cuts at: 2236 2361 2236 Size: 125 5270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4402 809 Size: 507 3086 1802 Fragments arranged by size: 3086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3731 5360 Size: 54 1425 50 275 18 1012 889 1629 Cuts at: 5360 8 Size: 43 Fragments arranged by size: 1629 1425 1012 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2842 1537 Size: 275 1030 4090 Fragments arranged by size: 4090 1030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 3325 1353 Size: 761 1211 3423 Fragments arranged by size: 3423 1211 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2417 3982 4356 4502 387 Size: 1064 138 828 1565 374 146 1280 Fragments arranged by size: 1565 1280 1064 828 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2625 3458 4055 4842 5110 1239 Size: 393 993 833 597 787 268 1524 Fragments arranged by size: 1524 993 833 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2021 2021 Size: 5395 EcoRI G'AATT_C Cuts at: 2704 3082 2704 Size: 378 5017 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2291 Size: 5395 FspI TGC'GCA Cuts at: 3901 3901 Size: 5395 HaeII r_GCGC'y Cuts at: 1678 4542 1678 Size: 2864 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4036 4068 4110 4142 1373 Size: 32 135 32 2464 32 42 32 2626 Fragments arranged by size: 2626 2464 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2739 2985 669 Size: 988 281 801 246 3079 Fragments arranged by size: 3079 988 801 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5395 HpaI GTT'AAC Cuts at: 2985 2985 Size: 5395 KpnI G_GTAC'C Cuts at: 2720 2720 Size: 5395 MfeI C'AATT_G Cuts at: 2994 2994 Size: 5395 MluI A'CGCG_T Cuts at: 5271 5271 Size: 5395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4509 4693 1305 Size: 3204 184 2007 Fragments arranged by size: 3204 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3573 Fragments arranged by size: 3573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3353 3712 3871 518 Size: 648 2187 359 159 2042 Fragments arranged by size: 2187 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2811 3488 4636 4881 1606 Size: 1205 677 1148 245 2120 Fragments arranged by size: 2120 1205 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4843 NdeI CA'TA_TG Cuts at: 387 387 Size: 5395 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5395 NotI GC'GGCC_GC Cuts at: 2842 2842 Size: 5395 NspI r_CATG'y Cuts at: 1132 1229 2753 4298 1132 Size: 97 1524 1545 2229 Fragments arranged by size: 2229 1545 1524 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5395 PciI A'CATG_T Cuts at: 4294 4294 Size: 5395 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5395 PmeI GTTT'AAAC Cuts at: 2699 2699 Size: 5395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2234 2266 3464 3496 4977 5009 2234 Size: 32 1198 32 1481 32 2620 Fragments arranged by size: 2620 1481 1198 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5395 PsiI TTA'TAA Cuts at: 2965 2965 Size: 5395 PspOMI G'GGCC_C Cuts at: 2020 2020 Size: 5395 PstI C_TGCA'G Cuts at: 830 2747 830 Size: 1917 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5395 SalI G'TCGA_C Cuts at: 1936 2737 1936 Size: 801 4594 SbfI CC_TGCA'GG Cuts at: 2747 2747 Size: 5395 ScaI AGT'ACT Cuts at: 3643 5355 3643 Size: 1712 3683 SexAI A'CCwGG_T Cuts at: 2232 2232 Size: 5395 SfcI C'TryA_G Cuts at: 826 1045 2453 2743 3878 4559 4750 826 Size: 219 1408 290 1135 681 191 1471 Fragments arranged by size: 1471 1408 1135 681 290 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5395 SmaI CCC'GGG Cuts at: 1872 2722 1872 Size: 850 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3499 4400 4662 820 Size: 197 926 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3186 493 Size: 2693 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5395 SphI G_CATG'C Cuts at: 1132 2753 1132 Size: 1621 3774 SspI AAT'ATT Cuts at: 5 52 3319 5 Size: 47 3267 2081 Fragments arranged by size: 3267 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5395 StyI C'CwwG_G Cuts at: 513 1065 2319 2664 2834 513 Size: 552 1254 345 170 3074 Fragments arranged by size: 3074 1254 552 345 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2046 2333 2398 3422 3439 3592 3777 Size: 1255 466 287 65 1024 17 153 185 Cuts at: 3777 4222 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1024 466 445 287 185 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3641 5353 96 Size: 274 80 33 51 1443 1664 1712 138 Fragments arranged by size: 1712 1664 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2598 2630 5355 5387 2598 Size: 32 2725 32 2606 Fragments arranged by size: 2725 2606 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5395 XbaI T'CTAG_A Cuts at: 2731 2731 Size: 5395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2181 2403 2181 Size: 222 5173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5395 XmaI C'CCGG_G Cuts at: 1870 2720 1870 Size: 850 4545 XmnI GAAnn'nnTTC Cuts at: 3524 3524 Size: 5395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3203 276 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI