################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AluI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuII RsrII SacI SacII SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 202 GTCGAC GT^[AC][GT]AC AflIII 445 ACGTGT A^C[GA][CT]GT AlwNI 235 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 54 GGGCCC GGGCC^C AspHI 905 GAGCAC G[AT]GC[AT]^C AvaII 468 GGTCC G^G[AT]CC BalI 368 TGGCCA TGG^CCA BamHI 677 GGATCC G^GATCC BcgI 245 CGAACCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 364 TACGTG [CT]AC^GT[GA] BsiEI 357 CGATCG CG[GA][CT]^CG BsiHKAI 905 GAGCAC G[AT]GC[AT]^C Bsp1407I 770 TGTACA T^GTACA BspMI 715 ACCTGC ACCTGC(4/8) BsrGI 770 TGTACA T^GTACA BstYI 677 GGATCC [GA]^GATC[CT] EcoT14I 58 CCAAGG C^C[AT][AT]GG FauI 788 CCCGC CCCGC(4/6) HgaI 291 GACGC GACGC(5/10) HincII 202 GTCGAC GT[CT]^[GA]AC HindII 202 GTCGAC GT[CT]^[GA]AC MflI 677 GGATCC [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MseI 851 TTAA T^TAA MwoI 231 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 15 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 357 CGATCG CGAT^CG SalI 202 GTCGAC G^TCGAC SapI 84 GCTCTTC GCTCTTC(1/4) SexAI 581 ACCAGGT A^CC[AT]GGT SspBI 770 TGTACA T^GTACA StuI 704 AGGCCT AGG^CCT StyI 58 CCAAGG C^C[AT][AT]GG TaqI 203 TCGA T^CGA TfiI 63 GATTC G^A[AT]TC Tru9I 851 TTAA T^TAA TthHB8I 203 TCGA T^CGA Van91I 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 677 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 174 GTAC GT^AC 771 GTAC AlwI 678 GATCC GGATC(4/5) 677 GGATC BanI 228 GGTGCC G^G[CT][GA]CC 892 GGTGCC BpmI 153 CTCCAG CTGGAG(16/14) 408 CTGGAG BsmAI 332 GTCTC GTCTC(1/5) 643 GTCTC BsmFI 741 GGGAC GGGAC(10/14) 786 GTCCC BspHI 401 TCATGA T^CATGA 510 TCATGA DpnII 358 GATC ^GATC 678 GATC EarI 85 CTCTTC CTCTTC(1/4) 745 CTCTTC HphI 107 TCACC GGTGA(8/7) 422 TCACC Ksp632I 85 CTCTTC CTCTTC(1/4) 745 CTCTTC MaeII 365 ACGT A^CGT 445 ACGT MboI 358 GATC ^GATC 678 GATC NdeII 358 GATC ^GATC 678 GATC RcaI 401 TCATGA T^CATGA 510 TCATGA RsaI 174 GTAC GT^AC 771 GTAC Sau3AI 358 GATC ^GATC 678 GATC Tsp45I 22 GTCAC ^GT[GC]AC 433 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AocI BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI EaeI Eco81I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboII MnlI MslI MvaI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI TspRI