(Circular) (Six-base) MAPSORT of: pFN21AE5913.seq Check: 471 from: 1 to: 5635 pFN21AE5913 5635 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 2729 Size: 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 2978 1937 Size: 271 770 4594 Fragments arranged by size: 4594 770 271 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 2450 4534 5511 1203 Size: 1247 2084 977 1327 Fragments arranged by size: 2084 1327 1247 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2249 1184 Size: 1065 4570 AlwNI CAG_nnn'CTG Cuts at: 2245 3378 4950 2245 Size: 1133 1572 2930 Fragments arranged by size: 2930 1572 1133 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 5635 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2944 3170 3322 5431 1493 Size: 1451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2960 1870 Size: 73 1017 4545 Fragments arranged by size: 4545 1017 73 BamHI G'GATC_C Cuts at: 2682 2965 3332 2682 Size: 283 367 4985 Fragments arranged by size: 4985 367 283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2897 2956 4411 618 Size: 325 217 627 446 664 59 1455 1842 Fragments arranged by size: 1842 1455 664 627 446 325 217 59 BanII G_rGCy'C Cuts at: 1391 2063 2091 2145 2954 1391 Size: 672 28 54 809 4072 Fragments arranged by size: 4072 809 672 54 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2239 2273 3826 3860 1903 Size: 34 302 34 1553 34 3678 Fragments arranged by size: 3678 1553 302 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 2063 2238 2902 3696 4852 1790 Size: 273 175 664 794 1156 2573 Fragments arranged by size: 2573 1156 794 664 273 175 BmgBI CAC'GTC Cuts at: 1318 2451 1318 Size: 1133 4502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2054 2924 3366 Size: 416 53 83 399 24 637 870 442 Cuts at: 3366 4324 442 Size: 958 1753 Fragments arranged by size: 1753 958 870 637 442 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2434 4295 1239 Size: 904 291 1861 2579 Fragments arranged by size: 2579 1861 904 291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4298 882 Size: 617 2799 2219 Fragments arranged by size: 2799 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 3426 493 Size: 711 1167 1055 2702 Fragments arranged by size: 2702 1167 1055 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5410 Fragments arranged by size: 5410 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2716 2819 2920 1186 Size: 883 647 103 101 3901 Fragments arranged by size: 3901 883 647 103 101 BseYI C'CCAG_C Cuts at: 2119 2633 4838 5334 2119 Size: 514 2205 496 2420 Fragments arranged by size: 2420 2205 514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5062 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2365 3085 3846 4874 1540 Size: 275 186 364 720 761 1028 2301 Fragments arranged by size: 2301 1028 761 720 364 275 186 BsiHKAI G_wGCw'C Cuts at: 2914 3696 3781 4852 2914 Size: 782 85 1071 3697 Fragments arranged by size: 3697 1071 782 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2091 2145 2238 2902 2914 2954 Size: 399 273 28 54 93 664 12 40 Cuts at: 2954 3696 3781 4852 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 273 93 85 54 40 28 12 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 2406 2515 3524 5254 2406 Size: 109 1009 1730 2787 Fragments arranged by size: 2787 1730 1009 109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 2990 844 Size: 1885 261 3489 Fragments arranged by size: 3489 1885 261 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 2775 96 Size: 2679 2956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2682 2965 3332 3717 3734 5186 5272 5284 Size: 710 283 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2555 2678 2802 2876 2903 2555 Size: 123 124 74 27 5287 Fragments arranged by size: 5287 124 123 74 27 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2197 487 Size: 683 56 971 3925 Fragments arranged by size: 3925 971 683 56 BtsI GCAGTG_nn' Cuts at: 2767 3151 3945 3965 2767 Size: 384 794 20 4437 Fragments arranged by size: 4437 794 384 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2433 2670 Size: 54 1425 50 275 18 543 60 237 Cuts at: 2670 3082 3971 5600 8 Size: 412 889 1629 43 Fragments arranged by size: 1629 1425 889 543 412 275 237 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2756 3565 1353 Size: 743 660 809 3423 Fragments arranged by size: 3423 809 743 660 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2434 3698 4295 5082 5350 1239 Size: 393 511 291 1264 597 787 268 1524 Fragments arranged by size: 1524 1264 787 597 511 393 291 268 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2385 2409 2417 2441 4276 Size: 32 135 32 813 24 8 24 1835 Cuts at: 4276 4308 4350 4382 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1835 813 135 42 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2209 2979 3225 669 Size: 988 281 271 770 246 3079 Fragments arranged by size: 3079 988 770 281 271 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3270 Fragments arranged by size: 3270 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2771 2777 3593 3952 4111 518 Size: 648 1008 597 6 816 359 159 2042 Fragments arranged by size: 2042 1008 816 648 597 359 159 6 MspA1I CmG'CkG Cuts at: 1606 3051 3728 4876 5121 1606 Size: 1445 677 1148 245 2120 Fragments arranged by size: 2120 1445 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 4498 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2024 1613 Size: 411 5224 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 5635 PstI C_TGCA'G Cuts at: 830 2987 830 Size: 2157 3478 PvuI CG_AT'CG Cuts at: 2001 2365 2001 Size: 364 5271 SalI G'TCGA_C Cuts at: 1936 2207 2977 1936 Size: 271 770 4594 Fragments arranged by size: 4594 770 271 SapI GCTCTTCn'nnn_ Cuts at: 2096 2096 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 5635 SfcI C'TryA_G Cuts at: 826 1045 2983 4118 4799 4990 826 Size: 219 1938 1135 681 191 1471 Fragments arranged by size: 1938 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3739 4640 4902 820 Size: 197 926 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 2063 3074 513 Size: 552 939 59 1011 3074 Fragments arranged by size: 3074 1011 939 552 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3662 3679 3832 4017 4462 325 Size: 1255 1067 1015 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1067 1015 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2775 3881 Size: 274 80 33 51 1443 201 597 1106 Cuts at: 3881 5593 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1106 597 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5595 5627 5595 Size: 32 5603 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI