################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AhdI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BglI BlnI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XcmI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 4149 TCCGGA T^CCGGA AflII 1816 CTTAAG C^TTAAG Alw44I 3802 GTGCAC G^TGCAC Aor51HI 181 AGCGCT AGC^GCT ApaI 1542 GGGCCC GGGCC^C ApaLI 3802 GTGCAC G^TGCAC AseI 3172 ATTAAT AT^TAAT AsnI 3172 ATTAAT AT^TAAT Asp700I 2530 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4245 TGGCCA TGG^CCA BanI 3972 GGCACC G^G[CT][GA]CC BbvCI 3985 CCTCAGC CCTCAGC(-5/-2) BcgI 918 CGACAAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1816 CTTAAG C^TTAAG BglII 141 AGATCT A^GATCT BlpI 1231 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1231 GCTGAGC GC^T[ACGT]AGC BsaAI 1132 TACGTG [CT]AC^GT[GA] BsaBI 1105 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4149 TCCGGA T^CCGGA BspEI 4149 TCCGGA T^CCGGA BspMI 3083 GCAGGT ACCTGC(4/8) CelII 1231 GCTGAGC GC^T[ACGT]AGC DraI 2050 TTTAAA TTT^AAA DrdI 4045 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 4245 TGGCCA [CT]^GGCC[GA] Eco47III 181 AGCGCT AGC^GCT EcoNI 4187 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1884 GAATTC G^AATTC EcoRV 787 GATATC GAT^ATC FauI 466 GCGGG CCCGC(4/6) MamI 1105 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4245 TGGCCA TGG^CCA MroI 4149 TCCGGA T^CCGGA MscI 4245 TGGCCA TGG^CCA NcoI 3659 CCATGG C^CATGG NdeI 1879 CATATG CA^TATG PflFI 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3172 ATTAAT AT^TAAT SapI 3124 GCTCTTC GCTCTTC(1/4) SexAI 3148 ACCTGGT A^CC[AT]GGT SmaI 153 CCCGGG CCC^GGG StuI 3318 AGGCCT AGG^CCT Tth111I 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 153 CCCGGG C^CCGGG XmaI 153 CCCGGG C^CCGGG XmnI 2530 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AflIII 1133 ACGTGT A^C[GA][CT]GT 2452 ACGTGT AocI 1477 CCTAAGG CC^T[ACGT]AGG 1537 CCTAAGG BciVI 3648 GGATAC GTATCC(6/5) 3663 GGATAC BclI 3029 TGATCA T^GATCA 3785 TGATCA BcnI 153 CCCGG CC^[GC]GG 154 CCGGG BsaI 1851 GAGACC GGTCTC(1/5) 1980 GGTCTC BsaWI 2159 ACCGGA [AT]^CCGG[AT] 4149 TCCGGA BsgI 3706 CTGCAC GTGCAG(16/14) 2797 GTGCAG BsmI 20 GAATGC GAATGC(1/-1) 3054 GCATTC BspHI 170 TCATGA T^CATGA 2526 TCATGA Bst1107I 913 GTATAC GTA^TAC 2293 GTATAC BstYI 141 AGATCT [GA]^GATC[CT] 2039 AGATCC BstZ17I 913 GTATAC GTA^TAC 2293 GTATAC Bsu36I 1477 CCTAAGG CC^T[ACGT]AGG 1537 CCTAAGG CfoI 182 GCGC GCG^C 3116 GCGC DraIII 1570 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3033 CACATTGTG Eco81I 1477 CCTAAGG CC^T[ACGT]AGG 1537 CCTAAGG EcoT14I 1421 CCAAGG C^C[AT][AT]GG 3659 CCATGG FbaI 3029 TGATCA T^GATCA 3785 TGATCA HaeII 181 AGCGCT [GA]GCGC^[CT] 3115 GGCGCT HhaI 182 GCGC GCG^C 3116 GCGC HinP1I 182 GCGC G^CGC 3116 GCGC HincII 1828 GTTGAC GT[CT]^[GA]AC 2428 GTTGAC HindII 1828 GTTGAC GT[CT]^[GA]AC 2428 GTTGAC HindIII 1859 AAGCTT A^AGCTT 2939 AAGCTT MflI 141 AGATCT [GA]^GATC[CT] 2039 AGATCC NciI 153 CCCGG CC^[GC]GG 154 CCGGG PaeR7I 3841 CTCGAG C^TCGAG 3967 CTCGAG PflMI 343 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4231 CCAAATCTTGG PpuMI 412 GGGACCT [GA]G^G[AT]CC[CT] 482 AGGACCC RcaI 170 TCATGA T^CATGA 2526 TCATGA SacI 3844 GAGCTC GAGCT^C 3949 GAGCTC StyI 1421 CCAAGG C^C[AT][AT]GG 3659 CCATGG Van91I 343 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4231 CCAAATCTTGG XhoI 3841 CTCGAG C^TCGAG 3967 CTCGAG XhoII 141 AGATCT [GA]^GATC[CT] 2039 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I