(Linear) (Six-base) MAPSORT of: KIEE9443.seq Check: 9557 from: 1 to: 4377 KIEE9443.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AccI GT'mk_AC Cuts at: 0 914 2294 2407 4377 Size: 914 1380 113 1970 Fragments arranged by size: 1970 1380 914 113 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1211 1667 1877 3279 3584 4097 4240 Size: 475 736 456 210 1402 305 513 143 Cuts at: 4240 4377 Size: 137 Fragments arranged by size: 1402 736 513 475 456 305 210 143 137 AfeI AGC'GCT Cuts at: 0 183 4377 Size: 183 4194 AflII C'TTAA_G Cuts at: 0 1816 4377 Size: 1816 2561 AflIII A'CryG_T Cuts at: 0 1133 2452 4377 Size: 1133 1319 1925 Fragments arranged by size: 1925 1319 1133 AleI CACnn'nnGTG Cuts at: 0 1210 2554 3713 4377 Size: 1210 1344 1159 664 Fragments arranged by size: 1344 1210 1159 664 AlwNI CAG_nnn'CTG Cuts at: 0 1737 3607 4075 4377 Size: 1737 1870 468 302 Fragments arranged by size: 1870 1737 468 302 ApaI G_GGCC'C Cuts at: 0 1546 4377 Size: 1546 2831 ApaLI G'TGCA_C Cuts at: 0 3802 4377 Size: 3802 575 ApoI r'AATT_y Cuts at: 0 506 691 1361 1884 2053 2680 4377 Size: 506 185 670 523 169 627 1697 Fragments arranged by size: 1697 670 627 523 506 185 169 AseI AT'TA_AT Cuts at: 0 3173 4377 Size: 3173 1204 AvaI C'yCGr_G Cuts at: 0 153 3841 3967 4377 Size: 153 3688 126 410 Fragments arranged by size: 3688 410 153 126 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2643 2676 4377 Size: 2643 33 1701 Fragments arranged by size: 2643 1701 33 BanI G'GyrC_C Cuts at: 0 3972 4377 Size: 3972 405 BanII G_rGCy'C Cuts at: 0 1546 3848 3953 4377 Size: 1546 2302 105 424 Fragments arranged by size: 2302 1546 424 105 BbsI GAAGACnn'nnnn_ Cuts at: 0 648 1804 3127 4181 4377 Size: 648 1156 1323 1054 196 Fragments arranged by size: 1323 1156 1054 648 196 BbvCI CC'TCA_GC Cuts at: 0 3986 4377 Size: 3986 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 907 941 4377 Size: 907 34 3436 Fragments arranged by size: 3436 907 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3642 3657 4377 Size: 3642 15 720 Fragments arranged by size: 3642 720 15 BclI T'GATC_A Cuts at: 0 3029 3785 4377 Size: 3029 756 592 Fragments arranged by size: 3029 756 592 BglII A'GATC_T Cuts at: 0 141 4377 Size: 141 4236 BlpI GC'TnA_GC Cuts at: 0 1232 4377 Size: 1232 3145 Bme1580I G_kGCm'C Cuts at: 0 1546 3806 4377 Size: 1546 2260 571 Fragments arranged by size: 2260 1546 571 BmgBI CAC'GTC Cuts at: 0 2256 4377 Size: 2256 2121 BmrI ACTGGGnnnn_n' Cuts at: 0 329 1581 2215 4377 Size: 329 1252 634 2162 Fragments arranged by size: 2162 1252 634 329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1189 1325 3967 4173 4377 Size: 1189 136 2642 206 204 Fragments arranged by size: 2642 1189 206 204 136 Bpu10I CC'TnA_GC Cuts at: 0 3986 4377 Size: 3986 391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 720 4377 Size: 720 3657 BsaI GGTCTCn'nnnn_ Cuts at: 0 1845 1986 4377 Size: 1845 141 2391 Fragments arranged by size: 2391 1845 141 BsaAI yAC'GTr Cuts at: 0 1134 4377 Size: 1134 3243 BsaBI GATnn'nnATC Cuts at: 0 1109 4377 Size: 1109 3268 BsaWI w'CCGG_w Cuts at: 0 2159 4149 4377 Size: 2159 1990 228 Fragments arranged by size: 2159 1990 228 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1043 1073 1931 1961 4377 Size: 1043 30 858 30 2416 Fragments arranged by size: 2416 1043 858 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1064 3193 4209 4377 Size: 1064 2129 1016 168 Fragments arranged by size: 2129 1064 1016 168 BseYI C'CCAG_C Cuts at: 0 1666 4377 Size: 1666 2711 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2818 3691 4377 Size: 2818 873 686 Fragments arranged by size: 2818 873 686 BsiHKAI G_wGCw'C Cuts at: 0 1294 3258 3378 3806 3848 3953 4377 Size: 1294 1964 120 428 42 105 424 Fragments arranged by size: 1964 1294 428 424 120 105 42 BsmI GAATG_Cn' Cuts at: 0 26 3054 4377 Size: 26 3028 1323 Fragments arranged by size: 3028 1323 26 Bsp1286I G_dGCh'C Cuts at: 0 1294 1546 3258 3378 3806 3848 3953 4377 Size: 1294 252 1712 120 428 42 105 424 Fragments arranged by size: 1712 1294 428 424 252 120 105 42 BspEI T'CCGG_A Cuts at: 0 4149 4377 Size: 4149 228 BspHI T'CATG_A Cuts at: 0 170 2526 4377 Size: 170 2356 1851 Fragments arranged by size: 2356 1851 170 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3074 4377 Size: 3074 1303 BsrDI GCAATG_nn' Cuts at: 0 2237 2672 3565 4377 Size: 2237 435 893 812 Fragments arranged by size: 2237 893 812 435 BstAPI GCAn_nnn'nTGC Cuts at: 0 937 1906 2773 4377 Size: 937 969 867 1604 Fragments arranged by size: 1604 969 937 867 BstXI CCAn_nnnn'nTGG Cuts at: 0 770 1635 2205 4377 Size: 770 865 570 2172 Fragments arranged by size: 2172 865 770 570 BstYI r'GATC_y Cuts at: 0 141 2039 4377 Size: 141 1898 2338 Fragments arranged by size: 2338 1898 141 BstZ17I GTA'TAC Cuts at: 0 915 2295 4377 Size: 915 1380 2082 Fragments arranged by size: 2082 1380 915 Bsu36I CC'TnA_GG Cuts at: 0 1478 1538 4377 Size: 1478 60 2839 Fragments arranged by size: 2839 1478 60 BtgI C'CryG_G Cuts at: 0 2616 3540 3659 4377 Size: 2616 924 119 718 Fragments arranged by size: 2616 924 718 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 162 4377 Size: 162 4215 BtsI GCAGTG_nn' Cuts at: 0 165 1106 2249 3891 4377 Size: 165 941 1143 1642 486 Fragments arranged by size: 1642 1143 941 486 165 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2874 2909 4377 Size: 2874 35 1468 Fragments arranged by size: 2874 1468 35 DraI TTT'AAA Cuts at: 0 2052 4377 Size: 2052 2325 DraIII CAC_nnn'GTG Cuts at: 0 1575 3038 4377 Size: 1575 1463 1339 Fragments arranged by size: 1575 1463 1339 DrdI GACnn_nn'nnGTC Cuts at: 0 4051 4377 Size: 4051 326 EaeI y'GGCC_r Cuts at: 0 4245 4377 Size: 4245 132 EarI CTCTTCn'nnn_ Cuts at: 0 494 890 3131 3208 3533 4377 Size: 494 396 2241 77 325 844 Fragments arranged by size: 2241 844 494 396 325 77 EcoICRI GAG'CTC Cuts at: 0 3846 3951 4377 Size: 3846 105 426 Fragments arranged by size: 3846 426 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1189 1211 1325 1667 1877 3279 3584 Size: 475 714 22 114 342 210 1402 305 Cuts at: 3584 3967 4097 4173 4240 4377 Size: 383 130 76 67 137 Fragments arranged by size: 1402 714 475 383 342 305 210 137 130 114 76 67 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 4191 4377 Size: 4191 186 EcoO109I rG'GnC_Cy Cuts at: 0 413 483 1282 1542 1543 4377 Size: 413 70 799 260 1 2834 Fragments arranged by size: 2834 799 413 260 70 1 EcoRI G'AATT_C Cuts at: 0 1884 4377 Size: 1884 2493 EcoRV GAT'ATC Cuts at: 0 789 4377 Size: 789 3588 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1286 4377 Size: 1286 3091 HaeII r_GCGC'y Cuts at: 0 185 3119 4377 Size: 185 2934 1258 Fragments arranged by size: 2934 1258 185 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 516 548 1931 1963 3183 3215 3901 3933 Size: 516 32 1383 32 1220 32 686 32 Cuts at: 3933 4377 Size: 444 Fragments arranged by size: 1383 1220 686 516 444 32 32 32 32 HincII GTy'rAC Cuts at: 0 1830 2430 4377 Size: 1830 600 1947 Fragments arranged by size: 1947 1830 600 HindIII A'AGCT_T Cuts at: 0 1859 2939 4377 Size: 1859 1080 1438 Fragments arranged by size: 1859 1438 1080 MfeI C'AATT_G Cuts at: 0 425 1016 1823 4377 Size: 425 591 807 2554 Fragments arranged by size: 2554 807 591 425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 175 2115 2874 3068 4377 Size: 175 1940 759 194 1309 Fragments arranged by size: 1940 1309 759 194 175 MscI TGG'CCA Cuts at: 0 4247 4377 Size: 4247 130 MslI CAynn'nnrTG Cuts at: 0 400 768 1210 2242 2554 3713 4377 Size: 400 368 442 1032 312 1159 664 Fragments arranged by size: 1159 1032 664 442 400 368 312 MspA1I CmG'CkG Cuts at: 0 870 1210 1231 1584 1728 1762 2905 3990 Size: 870 340 21 353 144 34 1143 1085 Cuts at: 3990 4362 4377 Size: 372 15 Fragments arranged by size: 1143 1085 870 372 353 340 144 34 21 15 NcoI C'CATG_G Cuts at: 0 3659 4377 Size: 3659 718 NdeI CA'TA_TG Cuts at: 0 1880 4377 Size: 1880 2497 NspI r_CATG'y Cuts at: 0 1805 2462 2729 4125 4230 4377 Size: 1805 657 267 1396 105 147 Fragments arranged by size: 1805 1396 657 267 147 105 PasI CC'CwG_GG Cuts at: 0 2412 3940 4377 Size: 2412 1528 437 Fragments arranged by size: 2412 1528 437 PflMI CCAn_nnn'nTGG Cuts at: 0 349 4237 4377 Size: 349 3888 140 Fragments arranged by size: 3888 349 140 PfoI T'CCnGG_A Cuts at: 0 348 2006 4189 4377 Size: 348 1658 2183 188 Fragments arranged by size: 2183 1658 348 188 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3360 3392 4377 Size: 3360 32 985 Fragments arranged by size: 3360 985 32 PpuMI rG'GwC_Cy Cuts at: 0 413 483 4377 Size: 413 70 3894 Fragments arranged by size: 3894 413 70 PsiI TTA'TAA Cuts at: 0 1031 1775 4377 Size: 1031 744 2602 Fragments arranged by size: 2602 1031 744 PspOMI G'GGCC_C Cuts at: 0 1542 4377 Size: 1542 2835 PspXI vC'TCGA_Gb Cuts at: 0 3841 3967 4377 Size: 3841 126 410 Fragments arranged by size: 3841 410 126 PstI C_TGCA'G Cuts at: 0 501 1101 1733 4377 Size: 501 600 632 2644 Fragments arranged by size: 2644 632 600 501 PvuII CAG'CTG Cuts at: 0 870 1210 1231 1584 1728 1762 2905 4362 Size: 870 340 21 353 144 34 1143 1457 Cuts at: 4362 4377 Size: 15 Fragments arranged by size: 1457 1143 870 353 340 144 34 21 15 SacI G_AGCT'C Cuts at: 0 3848 3953 4377 Size: 3848 105 424 Fragments arranged by size: 3848 424 105 SapI GCTCTTCn'nnn_ Cuts at: 0 3131 4377 Size: 3131 1246 SexAI A'CCwGG_T Cuts at: 0 3148 4377 Size: 3148 1229 SfcI C'TryA_G Cuts at: 0 137 497 1097 1298 1729 2623 4087 4377 Size: 137 360 600 201 431 894 1464 290 Fragments arranged by size: 1464 894 600 431 360 290 201 137 SmaI CCC'GGG Cuts at: 0 155 4377 Size: 155 4222 SmlI C'TyrA_G Cuts at: 0 699 1816 3841 3967 4377 Size: 699 1117 2025 126 410 Fragments arranged by size: 2025 1117 699 410 126 SphI G_CATG'C Cuts at: 0 2462 2729 4125 4230 4377 Size: 2462 267 1396 105 147 Fragments arranged by size: 2462 1396 267 147 105 SrfI GCCC'GGGC Cuts at: 0 155 4377 Size: 155 4222 SspI AAT'ATT Cuts at: 0 684 1380 2001 2735 2839 2854 3015 4377 Size: 684 696 621 734 104 15 161 1362 Fragments arranged by size: 1362 734 696 684 621 161 104 15 StuI AGG'CCT Cuts at: 0 3320 4377 Size: 3320 1057 StyI C'CwwG_G Cuts at: 0 1421 3659 4377 Size: 1421 2238 718 Fragments arranged by size: 2238 1421 718 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1563 2197 2544 2648 3096 4377 Size: 1563 634 347 104 448 1281 Fragments arranged by size: 1563 1281 634 448 347 104 TatI w'GTAC_w Cuts at: 0 2026 2182 4377 Size: 2026 156 2195 Fragments arranged by size: 2195 2026 156 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 623 655 4075 4107 4377 Size: 623 32 3420 32 270 Fragments arranged by size: 3420 623 270 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3755 4377 Size: 3755 622 XhoI C'TCGA_G Cuts at: 0 3841 3967 4377 Size: 3841 126 410 Fragments arranged by size: 3841 410 126 XmaI C'CCGG_G Cuts at: 0 153 4377 Size: 153 4224 XmnI GAAnn'nnTTC Cuts at: 0 2534 4377 Size: 2534 1843 Enzymes that do cut: AccI AcuI AfeI AflII AflIII AleI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SapI SexAI SfcI SmaI SmlI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AgeI AhdI AlfI AloI AscI AsiSI AvrII BamHI BbeI BfrBI BglI BmtI BplI BsaHI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII ClaI EagI EciI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SwaI XbaI XcmI ZraI