Restriction map for
pFN21AE9294
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRII EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvaI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 154 GCGG CCGC(-3/-1)
AlwNI 282 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 666 GGTCC G^G[AT]CC
BbsI 778 GTCTTC GAAGAC(2/6)
BbvCI 303 CCTCAGC CCTCAGC(-5/-2)
BbvI 627 GCAGC GCAGC(8/12)
BglII 119 AGATCT A^GATCT
BpmI 159 CTCCAG CTGGAG(16/14)
BpuAI 778 GTCTTC GAAGAC(2/6)
BsaAI 710 TACGTG [CT]AC^GT[GA]
BsaI 156 GGTCTC GGTCTC(1/5)
BseRI 366 GAGGAG GAGGAG(10/8)
BsrDI 511 GCAATG GCAATG(2/0)
BsrFI 271 ACCGGC [GA]^CCGG[CT]
BstF5I 831 CATCC GGATG(2/0)
BstXI 792 CCAAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 271 ACCGGC [GA]^CCGG[CT]
DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 96 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 133 GACCAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 633 CCATGG C^C[GA][CT]GG
EaeI 273 CGGCCA [CT]^GGCC[GA]
EarI 108 GAAGAG CTCTTC(1/4)
EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 576 GATATC GAT^ATC
Fnu4HI 627 GCAGC GC^[ACGT]GC
FokI 831 CATCC GGATG(9/13)
HapII 272 CCGG C^CGG
HpaII 272 CCGG C^CGG
HphI 105 GGTGA GGTGA(8/7)
ItaI 627 GCAGC GC^[ACGT]GC
Ksp632I 108 GAAGAG CTCTTC(1/4)
MaeII 711 ACGT A^CGT
MslI 384 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 807 CAGCTG C[AC]G^C[GT]G
MspI 272 CCGG C^CGG
NcoI 633 CCATGG C^CATGG
NheI 543 GCTAGC G^CTAGC
NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC
PflMI 95 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 807 CAGCTG CAG^CTG
SfaNI 624 GATGC GCATC(5/9)
TseI 627 GCAGC G^C[AT]GC
Tsp45I 142 GTCAC ^GT[GC]AC
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 95 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 29 CCATTCTAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 60 AAATTT [GA]^AATT[CT]
90 AAATTC
AlwI 652 GATCC GGATC(4/5)
643 GGATC
ApoI 60 AAATTT [GA]^AATT[CT]
90 AAATTC
BsaJI 633 CCATGG C^C[ACGT][ACGT]GG
662 CCAAGG
BsiYI 95 CCACCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BslI 95 CCACCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
834 CCTGATTTGGG
BsmAI 111 GAGAC GTCTC(1/5)
157 GTCTC
BsrI 287 ACTGG ACTGG(1/-1)
656 CCAGT
Cfr13I 403 GGGCC G^G[ACGT]CC
666 GGTCC
DdeI 304 CTCAG C^T[ACGT]AG
552 CTTAG
EcoT14I 633 CCATGG C^C[AT][AT]GG
662 CCAAGG
MwoI 275 GCCATTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
396 GCTGTTGGGGC
Sau96I 403 GGGCC G^G[ACGT]CC
666 GGTCC
StyI 633 CCATGG C^C[AT][AT]GG
662 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI BfaI BstYI Cac8I
DpnII HaeIII HinfI MaeI MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIII RsaI Sau3AI SmlI
TfiI Tru9I Tsp509I XhoII