################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FauI FseI FspI HaeII HaeIII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcyI 663 GACGCC G[GA]^CG[CT]C AflIII 377 ACATGT A^C[GA][CT]GT AlwI 502 GGATC GGATC(4/5) AlwNI 62 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 50 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 103 GAGCTC G[AT]GC[AT]^C BclI 173 TGATCA T^GATCA BsaHI 663 GACGCC G[GA]^CG[CT]C BsiHKAI 103 GAGCTC G[AT]GC[AT]^C BsmAI 531 GAGAC GTCTC(1/5) Bsp1407I 431 TGTACA T^GTACA BspLU11I 377 ACATGT A^CATGT BsrDI 702 CATTGC GCAATG(2/0) BsrFI 517 ACCGGC [GA]^CCGG[CT] BsrGI 431 TGTACA T^GTACA BsrI 630 ACTGG ACTGG(1/-1) BssKI 846 CCAGG ^CC[ACGT]GG BstNI 846 CCAGG CC^[AT]GG BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 502 GGATCT [GA]^GATC[CT] CfoI 114 GCGC GCG^C Cfr10I 517 ACCGGC [GA]^CCGG[CT] DsaI 839 CCATGG C^C[GA][CT]GG EcoRII 846 CCAGG ^CC[AT]GG EcoRV 247 GATATC GAT^ATC EcoT22I 327 ATGCAT ATGCA^T FbaI 173 TGATCA T^GATCA HapII 518 CCGG C^CGG HgaI 663 GACGC GACGC(5/10) HhaI 114 GCGC GCG^C Hin1I 663 GACGCC G[GA]^CG[CT]C HinP1I 114 GCGC G^CGC HpaII 518 CCGG C^CGG MflI 502 GGATCT [GA]^GATC[CT] MslI 150 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 109 CAGCTG C[AC]G^C[GT]G MspI 518 CCGG C^CGG MvaI 846 CCAGG CC^[AT]GG NcoI 839 CCATGG C^CATGG NdeI 395 CATATG CA^TATG NsiI 327 ATGCAT ATGCA^T NspI 377 ACATGT [GA]CATG^[CT] PflMI 768 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 109 CAGCTG CAG^CTG SacI 103 GAGCTC GAGCT^C ScrFI 846 CCAGG CC^[ACGT]GG SspBI 431 TGTACA T^GTACA TfiI 137 GAATC G^A[AT]TC Van91I 768 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 502 GGATCT [GA]^GATC[CT] XmnI 50 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 402 CCGC CCGC(-3/-1) 717 CCGC AvaII 740 GGTCC G^G[AT]CC 843 GGACC BpmI 106 CTCCAG CTGGAG(16/14) 606 CTCCAG Cac8I 666 GCCTGC GC[ACGT]^[ACGT]GC 706 GCCAGC Cfr13I 740 GGTCC G^G[ACGT]CC 843 GGACC EarI 118 CTCTTC CTCTTC(1/4) 580 CTCTTC EcoT14I 498 CCTTGG C^C[AT][AT]GG 839 CCATGG Ksp632I 118 CTCTTC CTCTTC(1/4) 580 CTCTTC PleI 333 GACTC GAGTC(4/5) 533 GACTC Sau96I 740 GGTCC G^G[ACGT]CC 843 GGACC SfaNI 494 GCATC GCATC(5/9) 710 GCATC SmlI 165 CTTGAG C^T[CT][GA]AG 642 CTTGAG StyI 498 CCTTGG C^C[AT][AT]GG 839 CCATGG Tsp509I 510 AATT ^AATT 626 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BanII BbvI BmyI BsaJI BsiYI BslI BsmI Bsp1286I BspMI BstF5I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII PstI RsaI Sau3AI SfcI Tru9I TseI Tsp45I TspRI