Restriction map for
pFN21AE6091
##################################
# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI FauI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII ItaI KasI KpnI KspI
MaeI MaeII MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TseI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 647 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 370 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 737 GGATAC GTATCC(6/5)
BclI 293 TGATCA T^GATCA
BsaBI 288 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 234 CCTTGG C^C[ACGT][ACGT]GG
BseRI 43 GAGGAG GAGGAG(10/8)
BspMI 646 GCAGGT ACCTGC(4/8)
BsrI 436 CCAGT ACTGG(1/-1)
BssKI 132 CCTGG ^CC[ACGT]GG
BstNI 132 CCTGG CC^[AT]GG
Cac8I 834 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 796 GGCCC G^G[ACGT]CC
EarI 52 GAAGAG CTCTTC(1/4)
EcoRII 132 CCTGG ^CC[AT]GG
EcoRV 321 GATATC GAT^ATC
EcoT14I 234 CCTTGG C^C[AT][AT]GG
FbaI 293 TGATCA T^GATCA
HindIII 518 AAGCTT A^AGCTT
Ksp632I 52 GAAGAG CTCTTC(1/4)
MamI 288 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 124 CCGCTG C[AC]G^C[GT]G
MvaI 132 CCTGG CC^[AT]GG
MwoI 385 GCAACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 395 CATATG CA^TATG
NlaIV 796 GGCCCC GG[ACGT]^[ACGT]CC
NspI 34 ACATGC [GA]CATG^[CT]
Sau96I 796 GGCCC G^G[ACGT]CC
ScrFI 132 CCTGG CC^[ACGT]GG
SfcI 906 CTACAG C^T[GA][CT]AG
StyI 234 CCTTGG C^C[AT][AT]GG
XmnI 370 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 124 CCGC CCGC(-3/-1)
832 CCGC
BanII 764 GGGCTC G[GA]GC[CT]^C
819 GGGCTC
BmyI 764 GGGCTC G[AGT]GC[ACT]^C
819 GGGCTC
BsiYI 161 CCATCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
755 CCACCTGTGGG
BslI 161 CCATCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
755 CCACCTGTGGG
BsmAI 751 GTCTC GTCTC(1/5)
861 GTCTC
Bsp1286I 764 GGGCTC G[AGT]GC[ACT]^C
819 GGGCTC
BsrDI 68 CATTGC GCAATG(2/0)
959 GCAATG
BstF5I 269 CATCC GGATG(2/0)
255 GGATG
EcoT22I 336 ATGCAT ATGCA^T
459 ATGCAT
FokI 269 CATCC GGATG(9/13)
255 GGATG
HaeIII 493 GGCC GG^CC
796 GGCC
HphI 158 TCACC GGTGA(8/7)
207 TCACC
MslI 159 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
942 CACAAAAATG
NsiI 336 ATGCAT ATGCA^T
459 ATGCAT
PleI 279 GACTC GAGTC(4/5)
859 GAGTC
TfiI 171 GAATC G^A[AT]TC
610 GATTC
Tsp45I 277 GTGAC ^GT[GC]AC
575 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI AluI BsmI DdeI DpnII
HinfI MaeIII MboI MboII MnlI
MseI NdeII NlaIII RsaI Sau3AI
SfaNI Tru9I Tsp509I TspRI