################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MamI MflI MluI MroI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 160 GTGCAC G^TGCAC AlwNI 241 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 876 CCTGAGG CC^T[ACGT]AGG ApaLI 160 GTGCAC G^TGCAC AvaI 668 CCCGGG C^[CT]CG[GA]G BbrPI 733 CACGTG CAC^GTG BbvI 373 GCTGC GCAGC(8/12) BciVI 573 GGATAC GTATCC(6/5) BglI 630 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 733 CACGTG [CT]AC^GT[GA] BsmI 712 GCATTC GAATGC(1/-1) BsoBI 668 CCCGGG C^[CT]CG[GA]G BspDI 114 ATCGAT AT^CGAT BssSI 225 CACGAG CACGAG(-5/-1) Bsu36I 876 CCTGAGG CC^T[ACGT]AGG ClaI 114 ATCGAT AT^CGAT DdeI 877 CTGAG C^T[ACGT]AG Eco81I 876 CCTGAGG CC^T[ACGT]AGG EcoT14I 426 CCTTGG C^C[AT][AT]GG EcoT22I 409 ATGCAT ATGCA^T Fnu4HI 373 GCTGC GC^[ACGT]GC HaeII 898 AGCGCC [GA]GCGC^[CT] ItaI 373 GCTGC GC^[ACGT]GC MaeII 734 ACGT A^CGT MaeIII 844 GTCAC ^GT[ACGT]AC MfeI 516 CAATTG C^AATTG MspA1I 502 CCGCTG C[AC]G^C[GT]G MunI 516 CAATTG C^AATTG NheI 432 GCTAGC G^CTAGC NsiI 409 ATGCAT ATGCA^T PflMI 315 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 733 CACGTG CAC^GTG PmlI 733 CACGTG CAC^GTG SapI 894 GAAGAGC GCTCTTC(1/4) SmaI 668 CCCGGG CCC^GGG SmlI 96 CTTGAG C^T[CT][GA]AG StyI 426 CCTTGG C^C[AT][AT]GG TseI 373 GCTGC G^C[AT]GC Tsp45I 844 GTCAC ^GT[GC]AC TspRI 399 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 315 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 464 CCACCAGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 668 CCCGGG C^CCGGG XmaI 668 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AfaI 180 GTAC GT^AC 600 GTAC AspHI 160 GTGCAC G[AT]GC[AT]^C 795 GAGCAC AvaII 262 GGACC G^G[AT]CC 297 GGTCC BalI 347 TGGCCA TGG^CCA 605 TGGCCA BanII 477 GGGCTC G[GA]GC[CT]^C 637 GGGCTC BcnI 668 CCCGG CC^[GC]GG 669 CCGGG BfaI 70 CTAG C^TAG 433 CTAG BpmI 26 CTCCAG CTGGAG(16/14) 781 CTCCAG BsaI 144 GGTCTC GGTCTC(1/5) 648 GGTCTC BsiHKAI 160 GTGCAC G[AT]GC[AT]^C 795 GAGCAC BsmFI 261 GGGAC GGGAC(10/14) 235 GTCCC BsrDI 663 CATTGC GCAATG(2/0) 125 GCAATG BsrI 561 ACTGG ACTGG(1/-1) 300 CCAGT BstF5I 412 CATCC GGATG(2/0) 642 CATCC BstXI 464 CCACCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 467 CCAGCATGGTGG DpnII 117 GATC ^GATC 684 GATC EarI 82 CTCTTC CTCTTC(1/4) 894 GAAGAG FokI 412 CATCC GGATG(9/13) 642 CATCC HapII 669 CCGG C^CGG 906 CCGG HpaII 669 CCGG C^CGG 906 CCGG Ksp632I 82 CTCTTC CTCTTC(1/4) 894 GAAGAG MaeI 70 CTAG C^TAG 433 CTAG MboI 117 GATC ^GATC 684 GATC MluNI 347 TGGCCA TGG^CCA 605 TGGCCA MscI 347 TGGCCA TGG^CCA 605 TGGCCA MspI 669 CCGG C^CGG 906 CCGG NciI 668 CCCGG CC^[GC]GG 669 CCGGG NdeII 117 GATC ^GATC 684 GATC NlaIV 261 GGGACC GG[ACGT]^[ACGT]CC 478 GGCTCC PstI 381 CTGCAG CTGCA^G 938 CTGCAG RsaI 180 GTAC GT^AC 600 GTAC Sau3AI 117 GATC ^GATC 684 GATC SfcI 381 CTGCAG C^T[GA][CT]AG 938 CTGCAG TaqI 115 TCGA T^CGA 524 TCGA Tsp509I 517 AATT ^AATT 957 AATT TthHB8I 115 TCGA T^CGA 524 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EaeI EcoRII HaeIII HhaI HinP1I HphI MboII MnlI MslI MvaI MwoI NlaIII Sau96I ScrFI SfaNI