(Circular) (Six-base) MAPSORT of: pFN21AE6063.seq Check: 3320 from: 1 to: 5665 pFN21AE6063 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 2894 1166 Size: 1728 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 2251 3408 4980 2251 Size: 1157 1572 2936 Fragments arranged by size: 2936 1572 1157 ApaLI G'TGCA_C Cuts at: 2165 3722 4878 2165 Size: 1557 1156 2952 Fragments arranged by size: 2952 1557 1156 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2673 2990 1870 Size: 73 730 317 4545 Fragments arranged by size: 4545 730 317 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2486 2646 2984 1391 Size: 1095 160 338 4072 Fragments arranged by size: 4072 1095 338 160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2572 3557 4767 2572 Size: 985 1210 3470 Fragments arranged by size: 3470 1210 985 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BfrBI ATG'CAT Cuts at: 2416 2416 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2641 4276 136 Size: 107 122 71 631 605 969 1635 1525 Fragments arranged by size: 1635 1525 969 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2169 3726 4882 1790 Size: 379 1557 1156 2573 Fragments arranged by size: 2573 1557 1156 379 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2441 1056 Size: 1385 4280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2016 2771 4325 1239 Size: 777 755 1554 2579 Fragments arranged by size: 2579 1554 777 755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2122 3790 4655 4953 2122 Size: 1668 865 298 2834 Fragments arranged by size: 2834 1668 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2155 2659 4328 882 Size: 617 656 504 1669 2219 Fragments arranged by size: 2219 1669 656 617 504 BsaAI yAC'GTr Cuts at: 493 1204 2740 3456 493 Size: 711 1536 716 2702 Fragments arranged by size: 2702 1536 716 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2283 2313 1178 Size: 30 165 30 880 30 4530 Fragments arranged by size: 4530 880 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2326 2455 4868 5364 2326 Size: 129 2413 496 2627 Fragments arranged by size: 2627 2413 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5092 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2804 3726 3811 4882 2169 Size: 635 922 85 1071 2952 Fragments arranged by size: 2952 1071 922 635 85 BsmI GAATG_Cn' Cuts at: 2717 3176 3269 2717 Size: 459 93 5113 Fragments arranged by size: 5113 459 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2486 2646 2804 2984 3726 3811 Size: 399 379 317 160 158 180 742 85 Cuts at: 3811 4882 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 317 180 160 158 85 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3020 844 Size: 2176 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 2667 4160 4334 65 Size: 1183 889 530 1493 174 1396 Fragments arranged by size: 1493 1396 1183 889 530 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 2230 3725 4737 2230 Size: 1495 1012 3158 Fragments arranged by size: 3158 1495 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2479 2476 Size: 3 5662 BstYI r'GATC_y Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660 Size: 1023 367 385 17 1452 86 12 346 Cuts at: 5660 1972 Size: 1977 Fragments arranged by size: 1977 1452 1023 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2882 2882 Size: 5665 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3975 3995 3181 Size: 794 20 4851 Fragments arranged by size: 4851 794 20 ClaI AT'CG_AT Cuts at: 1554 2120 1554 Size: 566 5099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2218 2253 2522 2557 529 Size: 35 1654 35 269 35 3637 Fragments arranged by size: 3637 1654 269 35 35 35 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 2610 2662 Size: 54 1425 50 275 18 522 258 52 Cuts at: 2662 3112 4001 5630 8 Size: 450 889 1629 43 Fragments arranged by size: 1629 1425 889 522 450 275 258 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2894 3595 1353 Size: 740 801 701 3423 Fragments arranged by size: 3423 801 740 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2722 4252 4626 4772 387 Size: 1064 138 1133 1530 374 146 1280 Fragments arranged by size: 1530 1280 1133 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2016 2771 3728 4325 5112 5380 1239 Size: 393 384 755 957 597 787 268 1524 Fragments arranged by size: 1524 957 787 755 597 393 384 268 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 2907 4812 1678 Size: 1229 1905 2531 Fragments arranged by size: 2531 1905 1229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373 Size: 32 135 32 2734 32 42 32 2626 Fragments arranged by size: 2734 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 2521 3264 2521 Size: 743 4922 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2354 2612 10 Size: 54 1425 343 522 258 3063 Fragments arranged by size: 3063 1425 522 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2160 2180 2474 2777 2894 3623 3982 Size: 648 994 20 294 303 117 729 359 Cuts at: 3982 4141 518 Size: 159 2042 Fragments arranged by size: 2042 994 729 648 359 303 294 159 117 20 MspA1I CmG'CkG Cuts at: 1606 2509 3081 3758 4906 5151 1606 Size: 903 572 677 1148 245 2120 Fragments arranged by size: 2120 1148 903 677 572 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5113 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 2437 1052 Size: 1385 4280 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NsiI A_TGCA'T Cuts at: 2418 2418 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 3023 4568 1132 Size: 97 1794 1545 2229 Fragments arranged by size: 2229 1794 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 1108 Size: 1218 4447 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PmlI CAC'GTG Cuts at: 2740 2740 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 2390 2947 3017 830 Size: 1560 557 70 3478 Fragments arranged by size: 3478 1560 557 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SapI GCTCTTCn'nnn_ Cuts at: 2894 2894 Size: 5665 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 2386 2943 3013 4148 4829 5020 826 Size: 219 1341 557 70 1135 681 191 1471 Fragments arranged by size: 1471 1341 1135 681 557 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2675 2992 1872 Size: 803 317 4545 Fragments arranged by size: 4545 803 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 2101 3769 4670 4932 820 Size: 197 926 158 1668 901 262 1553 Fragments arranged by size: 1668 1553 926 901 262 197 158 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SrfI GCCC'GGGC Cuts at: 2675 2675 Size: 5665 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5665 StyI C'CwwG_G Cuts at: 513 1065 2431 3104 513 Size: 552 1366 673 3074 Fragments arranged by size: 3074 1366 673 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3911 5623 Size: 274 80 33 51 1443 207 1727 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1727 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 5625 5657 2283 Size: 32 3310 32 2291 Fragments arranged by size: 3310 2291 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2673 2990 1870 Size: 803 317 4545 Fragments arranged by size: 4545 803 317 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI