################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstUI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 395 GGCGTC G[GA]^CG[CT]C AflIII 733 ACATGT A^C[GA][CT]GT AvaI 704 CCCGGG C^[CT]CG[GA]G AvaII 309 GGTCC G^G[AT]CC BfaI 73 CTAG C^TAG BglI 205 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 230 GCTTAGC GC^T[ACGT]AGC Bpu1102I 230 GCTTAGC GC^T[ACGT]AGC BsaHI 395 GGCGTC G[GA]^CG[CT]C BsmFI 820 GTCCC GGGAC(10/14) BsoBI 704 CCCGGG C^[CT]CG[GA]G Bsp1407I 324 TGTACA T^GTACA BspLU11I 733 ACATGT A^CATGT BspMI 870 ACCTGC ACCTGC(4/8) BsrFI 150 GCCGGC [GA]^CCGG[CT] BsrGI 324 TGTACA T^GTACA BstAPI 331 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 420 GGTCACC G^GT[ACGT]ACC BstPI 420 GGTCACC G^GT[ACGT]ACC BstXI 218 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 230 GCTTAGC GC^T[ACGT]AGC Cfr10I 150 GCCGGC [GA]^CCGG[CT] EarI 781 CTCTTC CTCTTC(1/4) EcoO65I 420 GGTCACC G^GT[ACGT]ACC FauI 899 CCCGC CCCGC(4/6) HgaI 396 GCGTC GACGC(5/10) Hin1I 395 GGCGTC G[GA]^CG[CT]C HphI 422 TCACC GGTGA(8/7) Ksp632I 781 CTCTTC CTCTTC(1/4) MaeI 73 CTAG C^TAG NaeI 150 GCCGGC GCC^GGC NdeI 293 CATATG CA^TATG NgoMIV 150 GCCGGC G^CCGGC PshAI 96 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 691 CTGCAG CTGCA^G SexAI 774 ACCTGGT A^CC[AT]GGT SmaI 704 CCCGGG CCC^GGG SspBI 324 TGTACA T^GTACA TfiI 346 GAATC G^A[AT]TC Tsp45I 421 GTCAC ^GT[GC]AC Tsp509I 22 AATT ^AATT XmaCI 704 CCCGGG C^CCGGG XmaI 704 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AluI 468 AGCT AG^CT 695 AGCT Alw44I 551 GTGCAC G^TGCAC 832 GTGCAC ApaLI 551 GTGCAC G^TGCAC 832 GTGCAC BcnI 704 CCCGG CC^[GC]GG 705 CCGGG BsaI 917 GAGACC GGTCTC(1/5) 778 GGTCTC BsgI 178 CTGCAC GTGCAG(16/14) 403 CTGCAC BsmAI 917 GAGAC GTCTC(1/5) 779 GTCTC BsrDI 675 CATTGC GCAATG(2/0) 137 GCAATG BstF5I 207 CATCC GGATG(2/0) 837 CATCC DdeI 231 CTTAG C^T[ACGT]AG 633 CTCAG DraII 277 GGGCCCC [GA]G^G[ACGT]CC[CT] 895 GGGGCCC DsaI 341 CCATGG C^C[GA][CT]GG 365 CCATGG EcoO109I 277 GGGCCCC [GA]G^G[ACGT]CC[CT] 895 GGGGCCC FokI 207 CATCC GGATG(9/13) 837 CATCC HapII 151 CCGG C^CGG 705 CCGG HpaII 151 CCGG C^CGG 705 CCGG MaeIII 421 GTCAC ^GT[ACGT]AC 584 GTAAC MboII 47 TCTTC GAAGA(8/7) 782 TCTTC MslI 558 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 745 CACATCTGTG MspI 151 CCGG C^CGG 705 CCGG NciI 704 CCCGG CC^[GC]GG 705 CCGGG NcoI 341 CCATGG C^CATGG 365 CCATGG NspI 327 ACATGC [GA]CATG^[CT] 733 ACATGT SfcI 242 CTATAG C^T[GA][CT]AG 691 CTGCAG StuI 722 AGGCCT AGG^CCT 761 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AfaI ApaI AspHI BalI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HinfI ItaI MboI MluNI MnlI MscI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TseI TspRI XcmI