(Circular) (Six-base) MAPSORT of: pFN21AE6058.seq Check: 6812 from: 1 to: 5665 pFN21AE6058 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2884 2884 Size: 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 2738 4564 5541 1203 Size: 1535 1826 977 1327 Fragments arranged by size: 1826 1535 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 2754 1166 Size: 1588 4077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaI G_GGCC'C Cuts at: 2118 2286 2905 2118 Size: 168 619 4878 Fragments arranged by size: 4878 619 168 ApaLI G'TGCA_C Cuts at: 2556 2837 3722 4878 2556 Size: 281 885 1156 3343 Fragments arranged by size: 3343 1156 885 281 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2709 2990 1870 Size: 73 766 281 4545 Fragments arranged by size: 4545 766 281 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2118 2286 2905 2984 1391 Size: 727 168 619 79 4072 Fragments arranged by size: 4072 727 619 168 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2216 4276 136 Size: 107 122 71 631 605 544 2060 1525 Fragments arranged by size: 2060 1525 631 605 544 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 2236 3082 2236 Size: 846 4819 Bme1580I G_kGCm'C Cuts at: 1790 2089 2118 2286 2560 2841 2905 3726 4882 Size: 299 29 168 274 281 64 821 1156 Cuts at: 4882 1790 Size: 2573 Fragments arranged by size: 2573 1156 821 299 281 274 168 64 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2945 2945 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4325 1239 Size: 3086 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2789 2916 4328 882 Size: 617 1290 127 1412 2219 Fragments arranged by size: 2219 1412 1290 617 127 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2401 3472 3854 4489 Size: 53 83 186 936 868 1071 382 635 Cuts at: 4489 275 Size: 1451 Fragments arranged by size: 1451 1071 936 868 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5440 Fragments arranged by size: 5440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2137 4868 5364 2137 Size: 2731 496 2438 Fragments arranged by size: 2731 2438 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 2393 1163 Size: 573 432 225 4435 Fragments arranged by size: 4435 573 432 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 2523 2560 2841 3726 3811 4882 2152 Size: 371 37 281 885 85 1071 2935 Fragments arranged by size: 2935 1071 885 371 281 85 37 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2118 2152 2286 2523 2560 2841 Size: 399 299 29 34 134 237 37 281 Cuts at: 2841 2905 2984 3726 3811 4882 1391 Size: 64 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 299 281 237 134 85 79 64 37 34 29 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2884 3020 844 Size: 2040 136 3489 Fragments arranged by size: 3489 2040 136 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 2679 4160 4334 65 Size: 1183 901 530 1481 174 1396 Fragments arranged by size: 1481 1396 1183 901 530 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 4309 4496 1700 Size: 110 345 2154 187 2869 Fragments arranged by size: 2869 2154 345 187 110 BsrGI T'GTAC_A Cuts at: 96 2329 96 Size: 2233 3432 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 2342 794 Size: 1548 4117 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 2425 1184 Size: 1241 4424 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660 Size: 1023 367 385 17 1452 86 12 346 Cuts at: 5660 1972 Size: 1977 Fragments arranged by size: 1977 1452 1023 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2346 2370 513 Size: 552 939 342 24 3808 Fragments arranged by size: 3808 939 552 342 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2150 2166 2180 2405 2498 2574 3181 3975 3995 Size: 16 14 225 93 76 607 794 20 Cuts at: 3995 2150 Size: 3820 Fragments arranged by size: 3820 794 607 225 93 76 20 16 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2367 2631 Size: 54 1425 50 275 18 378 159 264 Cuts at: 2631 3112 4001 5630 8 Size: 481 889 1629 43 Fragments arranged by size: 1629 1425 889 481 378 275 264 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2792 3595 1353 Size: 1439 803 3423 Fragments arranged by size: 3423 1439 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3728 4325 5112 5380 1239 Size: 393 2096 597 787 268 1524 Fragments arranged by size: 2096 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2283 2901 2283 Size: 618 5047 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373 Size: 32 135 32 2734 32 42 32 2626 Fragments arranged by size: 2734 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2877 4779 4963 1305 Size: 1572 1902 184 2007 Fragments arranged by size: 2007 1902 1572 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2369 2633 10 Size: 54 1425 343 378 159 264 3042 Fragments arranged by size: 3042 1425 378 343 264 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 2754 3623 3982 4141 518 Size: 648 1401 187 869 359 159 2042 Fragments arranged by size: 2042 1401 869 648 359 187 159 MspA1I CmG'CkG Cuts at: 1606 3081 3758 4906 5151 1606 Size: 1475 677 1148 245 2120 Fragments arranged by size: 2120 1475 1148 677 245 NaeI GCC'GGC Cuts at: 2157 2157 Size: 5665 NcoI C'CATG_G Cuts at: 513 1065 2004 2346 2370 513 Size: 552 939 342 24 3808 Fragments arranged by size: 3808 939 552 342 24 NdeI CA'TA_TG Cuts at: 387 2299 387 Size: 1912 3753 NgoMIV G'CCGG_C Cuts at: 2155 2155 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2336 2742 3023 4568 1132 Size: 97 1107 406 281 1545 2229 Fragments arranged by size: 2229 1545 1107 406 281 97 PasI CC'CwG_GG Cuts at: 1348 2255 1348 Size: 907 4758 PciI A'CATG_T Cuts at: 2738 4564 2738 Size: 1826 3839 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PfoI T'CCnGG_A Cuts at: 2931 2931 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2105 1613 Size: 492 5173 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PspOMI G'GGCC_C Cuts at: 2114 2282 2901 2114 Size: 168 619 4878 Fragments arranged by size: 4878 619 168 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2728 2760 2728 Size: 32 5633 PstI C_TGCA'G Cuts at: 830 2700 3017 830 Size: 1870 317 3478 Fragments arranged by size: 3478 1870 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2779 2779 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2247 2696 3013 4148 4829 5020 826 Size: 219 1202 449 317 1135 681 191 1471 Fragments arranged by size: 1471 1202 1135 681 449 317 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2711 2992 1872 Size: 839 281 4545 Fragments arranged by size: 4545 839 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4670 4932 820 Size: 197 926 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2729 2768 1517 Size: 1212 39 4414 Fragments arranged by size: 4414 1212 39 StyI C'CwwG_G Cuts at: 513 1065 2004 2346 2370 2454 2724 3104 513 Size: 552 939 342 24 84 270 380 3074 Fragments arranged by size: 3074 939 552 380 342 270 84 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2058 2940 3692 3709 3862 4047 4492 Size: 1255 478 882 752 17 153 185 445 Cuts at: 4492 325 Size: 1498 Fragments arranged by size: 1498 1255 882 752 478 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 3911 5623 Size: 274 80 33 51 1443 352 1582 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1712 1582 1443 352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 5633 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2437 2641 2125 Size: 312 204 5149 Fragments arranged by size: 5149 312 204 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2709 2990 1870 Size: 839 281 4545 Fragments arranged by size: 4545 839 281 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI