################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AciI 795 CCGC CCGC(-3/-1) AgeI 362 ACCGGT A^CCGGT Asp700I 31 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 118 CTCGGG C^[CT]CG[GA]G AvrII 765 CCTAGG C^CTAGG BamHI 638 GGATCC G^GATCC BanII 896 GGGCTC G[GA]GC[CT]^C BlnI 765 CCTAGG C^CTAGG BsaBI 246 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 765 CCTAGG C^C[ACGT][ACGT]GG BsaWI 362 ACCGGT [AT]^CCGG[AT] BsmAI 742 GTCTC GTCTC(1/5) BsmFI 626 GTCCC GGGAC(10/14) BsoBI 118 CTCGGG C^[CT]CG[GA]G Bsp1407I 830 TGTACA T^GTACA BspMI 125 ACCTGC ACCTGC(4/8) BsrFI 362 ACCGGT [GA]^CCGG[CT] BsrGI 830 TGTACA T^GTACA BsrI 634 ACTGG ACTGG(1/-1) BstEII 840 GGTCACC G^GT[ACGT]ACC BstPI 840 GGTCACC G^GT[ACGT]ACC BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 638 GGATCC [GA]^GATC[CT] Cac8I 286 GCCTGC GC[ACGT]^[ACGT]GC CfoI 610 GCGC GCG^C Cfr10I 362 ACCGGT [GA]^CCGG[CT] DpnII 639 GATC ^GATC DraII 624 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 586 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 624 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 840 GGTCACC G^GT[ACGT]ACC EcoT14I 765 CCTAGG C^C[AT][AT]GG FauI 794 CCCGC CCCGC(4/6) HapII 363 CCGG C^CGG HhaI 610 GCGC GCG^C HinP1I 610 GCGC G^CGC HinfI 154 GACTC G^A[ACGT]TC HpaII 363 CCGG C^CGG MamI 246 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 639 GATC ^GATC MflI 638 GGATCC [GA]^GATC[CT] MspI 363 CCGG C^CGG NdeII 639 GATC ^GATC NspI 675 ACATGC [GA]CATG^[CT] PinAI 362 ACCGGT A^CCGGT PleI 154 GACTC GAGTC(4/5) PpuMI 624 AGGTCCC [GA]G^G[AT]CC[CT] Sau3AI 639 GATC ^GATC SexAI 104 ACCTGGT A^CC[AT]GGT SfcI 867 CTATAG C^T[GA][CT]AG SmlI 807 CTCAAG C^T[CT][GA]AG SspBI 830 TGTACA T^GTACA StyI 765 CCTAGG C^C[AT][AT]GG XhoII 638 GGATCC [GA]^GATC[CT] XmnI 31 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 23 AAATTT [GA]^AATT[CT] 31 GAATTT AluI 574 AGCT AG^CT 690 AGCT AlwI 639 GATCC GGATC(4/5) 638 GGATC ApoI 23 AAATTT [GA]^AATT[CT] 31 GAATTT AspHI 115 GTGCTC G[AT]GC[AT]^C 455 GTGCTC AvaII 446 GGACC G^G[AT]CC 625 GGTCC BalI 140 TGGCCA TGG^CCA 371 TGGCCA BbvI 612 GCTGC GCAGC(8/12) 787 GCTGC BfaI 436 CTAG C^TAG 766 CTAG BsgI 388 CTGCAC GTGCAG(16/14) 504 CTGCAC BsiHKAI 115 GTGCTC G[AT]GC[AT]^C 455 GTGCTC Cfr13I 446 GGACC G^G[ACGT]CC 625 GGTCC EaeI 140 TGGCCA [CT]^GGCC[GA] 371 TGGCCA Fnu4HI 612 GCTGC GC^[ACGT]GC 787 GCTGC ItaI 612 GCTGC GC^[ACGT]GC 787 GCTGC MaeI 436 CTAG C^TAG 766 CTAG MluNI 140 TGGCCA TGG^CCA 371 TGGCCA MscI 140 TGGCCA TGG^CCA 371 TGGCCA Sau96I 446 GGACC G^G[ACGT]CC 625 GGTCC SfaNI 439 GCATC GCATC(5/9) 724 GCATC TseI 612 GCTGC G^C[AT]GC 787 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI BmyI BsiYI BslI Bsp1286I BssKI BstF5I BstNI DdeI EcoRII FokI HaeIII HphI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV RsaI ScrFI Tru9I Tsp45I Tsp509I TspRI