Restriction map for pFN21AE6056
 (Circular) (Six-base) MAPSORT of: pFN21AE6056.seq  Check: 8882  from: 1  to: 5662



pFN21AE6056  5662 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3472     278 
   Size:         53      83     186     936    1936    2468

  Fragments arranged by size: 

                2468    1936     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3005    1937 
   Size:       1068    4594
 
Acc65I G'GTAC_C

Cuts at:   2983    2983 
   Size:       5662
 
AclI AA'CG_TT

Cuts at:   1563    3789    4162    1563 
   Size:       2226     373    3063

  Fragments arranged by size: 

                3063    2226     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3725    5109    5377    1632 
   Size:       2093    1384     268    1917

  Fragments arranged by size: 

                2093    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5465
 
AflIII A'CryG_T

Cuts at:   1203    4561    5538    1203 
   Size:       3358     977    1327

  Fragments arranged by size: 

                3358    1327     977
 
AgeI A'CCGG_T

Cuts at:   2367    4493    2367 
   Size:       2126    3536
 
AhdI GACnn_n'nnGTC

Cuts at:   4391    4391 
   Size:       5662
 
AleI CACnn'nnGTG

Cuts at:   1166    2424    2814    1166 
   Size:       1258     390    4014

  Fragments arranged by size: 

                4014    1258     390
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5662
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2421    2453    2421 
   Size:         32    5630
 
AlwNI CAG_nnn'CTG

Cuts at:   3405    4977    3405 
   Size:       1572    4090
 
ApaLI G'TGCA_C

Cuts at:   3719    4875    3719 
   Size:       1156    4506
 
ApoI r'AATT_y

Cuts at:   1493    2028    2036    2971    3197    3349    5458    1493 
   Size:        535       8     935     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     935     535     226     152       8
 
AseI AT'TA_AT

Cuts at:    160    1032    4216     160 
   Size:        872    3184    1606

  Fragments arranged by size: 

                3184    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5662
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2123    2987    1870 
   Size:         73     180     864    4545

  Fragments arranged by size: 

                4545     864     180      73
 
AvrII C'CTAG_G

Cuts at:   2770    2770 
   Size:       5662
 
BamHI G'GATC_C

Cuts at:   2643    2992    3359    2643 
   Size:        349     367    4946

  Fragments arranged by size: 

                4946     367     349
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2983    4438     618 
   Size:        325     217     627    1196    1455    1842

  Fragments arranged by size: 

                1842    1455    1196     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2905    2981    1391 
   Size:       1514      76    4072

  Fragments arranged by size: 

                4072    1514      76
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4691
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3853    3887    1903 
   Size:         34    1916      34    3678

  Fragments arranged by size: 

                3678    1916      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3554    4764    3554 
   Size:       1210    4452
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5662
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4273     136 
   Size:        107     122      71     631     605    2601    1525

  Fragments arranged by size: 

                2601    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5657    5657 
   Size:       5662
 
BlpI GC'TnA_GC

Cuts at:   3079    3079 
   Size:       5662
 
Bme1580I G_kGCm'C

Cuts at:   1790    2960    3723    4879    1790 
   Size:       1170     763    1156    2573

  Fragments arranged by size: 

                2573    1170    1156     763
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5662
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2649    2954    3393 
   Size:        416      53      83     399      24    1232     305     439

Cuts at:   3393    4351     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1232     958     439     416     399     305      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5662
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4322    1239 
   Size:       3083    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2697    2697 
   Size:       5662
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2797    3787    4652    4950    2797 
   Size:        990     865     298    3509

  Fragments arranged by size: 

                3509     990     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4325     882 
   Size:        617    2826    2219

  Fragments arranged by size: 

                2826    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3453     493 
   Size:        711    2249    2702

  Fragments arranged by size: 

                2702    2249     711
 
BsaBI GATnn'nnATC

Cuts at:   2255    5656    2255 
   Size:       3401    2261
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3469    3851    4486     275 
   Size:         53      83     186     936    1936     382     635    1451

  Fragments arranged by size: 

                1936    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2367    4095    4493    4767    4914    5325    2367 
   Size:       1728     398     274     147     411    2704

  Fragments arranged by size: 

                2704    1728     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5437

  Fragments arranged by size: 

                5437     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5662
 
BseYI C'CCAG_C

Cuts at:   2620    4865    5361    2620 
   Size:       2245     496    2921

  Fragments arranged by size: 

                2921    2245     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2378    2494    1163 
   Size:        573     642     116    4331

  Fragments arranged by size: 

                4331     642     573     116
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3112    3873    4901    1540 
   Size:        275     186    1111     761    1028    2301

  Fragments arranged by size: 

                2301    1111    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2124    2464    3723    3808    4879    2124 
   Size:        340    1259      85    1071    2907

  Fragments arranged by size: 

                2907    1259    1071     340      85
 
BsmI GAATG_Cn'

Cuts at:   3173    3266    3173 
   Size:         93    5569
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2124    2464    2905    2960    2981    3723    3808 
   Size:        399     334     340     441      55      21     742      85

Cuts at:   3808    4879    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     441     399     340     334      85
                  55      21
 
BspEI T'CCGG_A

Cuts at:   5325    5325 
   Size:       5662
 
BspHI T'CATG_A

Cuts at:   3551    5281    3551 
   Size:       1730    3932
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2139    3017     844 
   Size:       1295     878    3489

  Fragments arranged by size: 

                3489    1295     878
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3549    1394 
   Size:        429    1726    3507

  Fragments arranged by size: 

                3507    1726     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4157    4331      65 
   Size:       1183    2909     174    1396

  Fragments arranged by size: 

                2909    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2367    4306    4493    1700 
   Size:        110     557    1939     187    2869

  Fragments arranged by size: 

                2869    1939     557     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2835      96 
   Size:       2739    2923
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5662
 
BssSI C'ACGA_G

Cuts at:   3722    4734    3722 
   Size:       1012    4650
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5662
 
BstBI TT'CG_AA

Cuts at:   4466    4466 
   Size:       5662
 
BstEII G'GTnAC_C

Cuts at:   1184    2845    1184 
   Size:       1661    4001
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2110    2110 
   Size:       5662
 
BstYI r'GATC_y

Cuts at:   1972    2643    2992    3359    3744    3761    5213    5299    5311 
   Size:        671     349     367     385      17    1452      86      12

Cuts at:   5311    5657    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     671     385     367     349     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4171

  Fragments arranged by size: 

                4171     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4923

  Fragments arranged by size: 

                4923     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3178    3972    3992    3178 
   Size:        794      20    4848

  Fragments arranged by size: 

                4848     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5662
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5627
 
DraI TTT'AAA

Cuts at:   2966    2966 
   Size:       5662
 
DraIII CAC_nnn'GTG

Cuts at:   2596    2596 
   Size:       5662
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4669     809 
   Size:        507    3353    1802

  Fragments arranged by size: 

                3353    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2145    2376    3109 
   Size:         54    1425      50     275      18     315     231     733

Cuts at:   3109    3998    5627       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     733     315     275     231      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3109    1537 
   Size:        275    1297    4090

  Fragments arranged by size: 

                4090    1297     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3592    1353 
   Size:       2239    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4249    4623    4769     387 
   Size:       1064     138    2660     374     146    1280

  Fragments arranged by size: 

                2660    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3725    4322    5109    5377    1239 
   Size:        393    2093     597     787     268    1524

  Fragments arranged by size: 

                2093    1524     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2630    2630 
   Size:       5662
 
EcoRI G'AATT_C

Cuts at:   2971    3349    2971 
   Size:        378    5284
 
FspI TGC'GCA

Cuts at:   4168    4168 
   Size:       5662
 
HaeII r_GCGC'y

Cuts at:   1678    4809    1678 
   Size:       3131    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4303    4335    4377    4409    1373 
   Size:         32     135      32    2731      32      42      32    2626

  Fragments arranged by size: 

                2731    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3006    3252     669 
   Size:        988     281    1068     246    3079

  Fragments arranged by size: 

                3079    1068     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5662
 
HpaI GTT'AAC

Cuts at:   3252    3252 
   Size:       5662
 
KpnI G_GTAC'C

Cuts at:   2987    2987 
   Size:       5662
 
MfeI C'AATT_G

Cuts at:   3261    3261 
   Size:       5662
 
MluI A'CGCG_T

Cuts at:   5538    5538 
   Size:       5662
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2220    2584    4776    4960    1305 
   Size:        915     364    2192     184    2007

  Fragments arranged by size: 

                2192    2007     915     364     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2147    2378      10 
   Size:         54    1425     343     315     231    3294

  Fragments arranged by size: 

                3294    1425     343     315     231      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2177    2303    2424    2814    2852    3620    3979 
   Size:        648    1011     126     121     390      38     768     359

Cuts at:   3979    4138     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1011     768     648     390     359     159     126
                 121      38
 
MspA1I CmG'CkG

Cuts at:   1606    3078    3755    4903    5148    1606 
   Size:       1472     677    1148     245    2120

  Fragments arranged by size: 

                2120    1472    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4171

  Fragments arranged by size: 

                4171     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5662
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5662
 
NotI GC'GGCC_GC

Cuts at:   3109    3109 
   Size:       5662
 
NspI r_CATG'y

Cuts at:   1132    1229    2684    3020    4565    1132 
   Size:         97    1455     336    1545    2229

  Fragments arranged by size: 

                2229    1545    1455     336      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5662
 
PciI A'CATG_T

Cuts at:   4561    4561 
   Size:       5662
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5662
 
PfoI T'CCnGG_A

Cuts at:   2447    2447 
   Size:       5662
 
PmeI GTTT'AAAC

Cuts at:   2966    2966 
   Size:       5662
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3731    3763    5244    5276    3731 
   Size:         32    1481      32    4117

  Fragments arranged by size: 

                4117    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2630    2630 
   Size:       5662
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5662
 
PsiI TTA'TAA

Cuts at:   3232    3232 
   Size:       5662
 
PstI C_TGCA'G

Cuts at:    830    3014     830 
   Size:       2184    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5662
 
SalI G'TCGA_C

Cuts at:   1936    3004    1936 
   Size:       1068    4594
 
SbfI CC_TGCA'GG

Cuts at:   3014    3014 
   Size:       5662
 
ScaI AGT'ACT

Cuts at:   3910    5622    3910 
   Size:       1712    3950
 
SexAI A'CCwGG_T

Cuts at:   2109    2109 
   Size:       5662
 
SfcI C'TryA_G

Cuts at:    826    1045    2872    3010    4145    4826    5017     826 
   Size:        219    1827     138    1135     681     191    1471

  Fragments arranged by size: 

                1827    1471    1135     681     219     191     138
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5662
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5662
 
SmaI CCC'GGG

Cuts at:   1872    2989    1872 
   Size:       1117    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2812    3766    4667    4929     820 
   Size:        197     926     869     954     901     262    1553

  Fragments arranged by size: 

                1553     954     926     901     869     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3453     493 
   Size:       2960    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5662
 
SphI G_CATG'C

Cuts at:   1132    3020    1132 
   Size:       1888    3774
 
SspI AAT'ATT

Cuts at:      5      52    3586       5 
   Size:         47    3534    2081

  Fragments arranged by size: 

                3534    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5662
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2770    3101     513 
   Size:        552     939     766     331    3074

  Fragments arranged by size: 

                3074     939     766     552     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2612    3689    3706    3859    4044    4489     325 
   Size:       1255    1032    1077      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1077    1032     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2181    2706    2835 
   Size:        274      80      33      51    1443     204     525     129

Cuts at:   2835    3908    5620      96 
   Size:       1073    1712     138

  Fragments arranged by size: 

                1712    1443    1073     525     274     204     138     129
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2221    2253    2443    2475    5622    5654    2221 
   Size:         32     190      32    3147      32    2229

  Fragments arranged by size: 

                3147    2229     190      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5662
 
XbaI T'CTAG_A

Cuts at:   2998    2998 
   Size:       5662
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5662
 
XmaI C'CCGG_G

Cuts at:   1870    2987    1870 
   Size:       1117    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2040    3791    2040 
   Size:       1751    3911
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3470     276 
   Size:         53      83     186     936    1936    2468

  Fragments arranged by size: 

                2468    1936     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI     BaeI     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
     XcmI
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