################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 908 GTCGAC GT^[AC][GT]AC AhdI 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 160 GGGCTC G[GA]GC[CT]^C BbsI 598 GTCTTC GAAGAC(2/6) BfaI 413 CTAG C^TAG BpuAI 598 GTCTTC GAAGAC(2/6) BseRI 323 GAGGAG GAGGAG(10/8) BsgI 388 CTGCAC GTGCAG(16/14) BsmAI 57 GTCTC GTCTC(1/5) BspHI 404 TCATGA T^CATGA BspMI 756 ACCTGC ACCTGC(4/8) BsrDI 427 GCAATG GCAATG(2/0) BsrFI 380 GCCGGC [GA]^CCGG[CT] BssSI 725 CTCGTG CACGAG(-5/-1) BstXI 236 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 380 GCCGGC [GA]^CCGG[CT] DraII 787 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 823 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 894 GAAGAG CTCTTC(1/4) EcoO109I 787 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 209 ATGCAT ATGCA^T HapII 381 CCGG C^CGG HincII 908 GTCGAC GT[CT]^[GA]AC HindII 908 GTCGAC GT[CT]^[GA]AC HpaII 381 CCGG C^CGG HphI 140 TCACC GGTGA(8/7) Ksp632I 894 GAAGAG CTCTTC(1/4) MaeI 413 CTAG C^TAG MaeIII 25 GTGAC ^GT[ACGT]AC MseI 94 TTAA T^TAA MspI 381 CCGG C^CGG NaeI 380 GCCGGC GCC^GGC NgoMIV 380 GCCGGC G^CCGGC NlaIV 231 GGCTCC GG[ACGT]^[ACGT]CC NsiI 209 ATGCAT ATGCA^T PflFI 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 404 TCATGA T^CATGA SalI 908 GTCGAC G^TCGAC SfcI 279 CTACAG C^T[GA][CT]AG SmlI 59 CTCAAG C^T[CT][GA]AG TaqI 909 TCGA T^CGA TfiI 846 GAATC G^A[AT]TC Tru9I 94 TTAA T^TAA Tsp45I 25 GTGAC ^GT[GC]AC Tth111I 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 909 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 492 CCGC AcsI 31 GAATTT [GA]^AATT[CT] 123 AAATTT ApoI 31 GAATTT [GA]^AATT[CT] 123 AAATTT AvaII 480 GGTCC G^G[AT]CC 754 GGACC BbvI 682 GCAGC GCAGC(8/12) 287 GCTGC BmyI 160 GGGCTC G[AGT]GC[ACT]^C 777 GTGCCC Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C 777 GTGCCC DpnII 90 GATC ^GATC 831 GATC EcoT14I 42 CCTTGG C^C[AT][AT]GG 318 CCTTGG Fnu4HI 287 GCTGC GC^[ACGT]GC 682 GCAGC ItaI 287 GCTGC GC^[ACGT]GC 682 GCAGC MboI 90 GATC ^GATC 831 GATC MspA1I 517 CAGCTG C[AC]G^C[GT]G 797 CAGCTG NdeII 90 GATC ^GATC 831 GATC PleI 51 GACTC GAGTC(4/5) 906 GAGTC PvuII 517 CAGCTG CAG^CTG 797 CAGCTG Sau3AI 90 GATC ^GATC 831 GATC StyI 42 CCTTGG C^C[AT][AT]GG 318 CCTTGG TseI 287 GCTGC G^C[AT]GC 682 GCAGC XcmI 236 CCAGGATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 467 CCATTATCCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AflIII AluI AlwNI BsaJI BsiYI BslI BspLU11I BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FokI HaeIII HinfI MboII MnlI MslI MvaI MwoI NlaIII NspI Sau96I ScrFI SfaNI Tsp509I TspRI