################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MluI MroI MspI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] AfaI 180 GTAC GT^AC AlwNI 605 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTT [GA]^AATT[CT] BamHI 649 GGATCC G^GATCC BanII 930 GGGCTC G[GA]GC[CT]^C BbvCI 694 GCTGAGG CCTCAGC(-5/-2) BclI 257 TGATCA T^GATCA BsmAI 745 GTCTC GTCTC(1/5) BsmFI 250 GGGAC GGGAC(10/14) BsmI 712 GCATTC GAATGC(1/-1) BsrDI 125 GCAATG GCAATG(2/0) BssSI 40 CTCGTG CACGAG(-5/-1) BstYI 649 GGATCC [GA]^GATC[CT] Cac8I 432 GCTGGC GC[ACGT]^[ACGT]GC DsaI 353 CCATGG C^C[GA][CT]GG FbaI 257 TGATCA T^GATCA MfeI 20 CAATTG C^AATTG MflI 649 GGATCC [GA]^GATC[CT] MseI 118 TTAA T^TAA MunI 20 CAATTG C^AATTG NcoI 353 CCATGG C^CATGG NdeI 359 CATATG CA^TATG PflMI 732 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 18 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 180 GTAC GT^AC SmlI 876 CTCAAG C^T[CT][GA]AG Tru9I 118 TTAA T^TAA Van91I 732 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 649 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 525 GAACCACTTC AvaII 251 GGACC G^G[AT]CC 923 GGACC BglI 349 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 427 GCCCAGCTGGC BpmI 208 CTGGAG CTGGAG(16/14) 592 CTGGAG BsaAI 370 TACGTG [CT]AC^GT[GA] 774 CACGTA BsgI 161 CTGCAC GTGCAG(16/14) 899 CTGCAC BstXI 101 CCATTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 239 CCAAGATGCTGG DraII 250 GGGACCC [GA]G^G[ACGT]CC[CT] 922 AGGACCC EcoO109I 250 GGGACCC [GA]G^G[ACGT]CC[CT] 922 AGGACCC EcoT14I 285 CCTTGG C^C[AT][AT]GG 353 CCATGG HinfI 157 GACTC G^A[ACGT]TC 846 GACTC HphI 522 GGTGA GGTGA(8/7) 843 GGTGA MaeII 371 ACGT A^CGT 775 ACGT PleI 157 GACTC GAGTC(4/5) 846 GACTC PpuMI 250 GGGACCC [GA]G^G[AT]CC[CT] 922 AGGACCC StyI 285 CCTTGG C^C[AT][AT]GG 353 CCATGG Tsp509I 21 AATT ^AATT 32 AATT XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 525 GAACCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI BalI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI