################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StyI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### AccI 618 GTCGAC GT^[AC][GT]AC AciI 116 CCGC CCGC(-3/-1) AcsI 434 AAATTT [GA]^AATT[CT] AcyI 800 GACGCC G[GA]^CG[CT]C AflIII 77 ACATGT A^C[GA][CT]GT Alw44I 231 GTGCAC G^TGCAC AocI 241 CCTCAGG CC^T[ACGT]AGG ApaLI 231 GTGCAC G^TGCAC ApoI 434 AAATTT [GA]^AATT[CT] AspHI 231 GTGCAC G[AT]GC[AT]^C AspI 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 69 GGACC G^G[AT]CC BbrPI 228 CACGTG CAC^GTG BbvI 164 GCAGC GCAGC(8/12) BciVI 818 GTATCC GTATCC(6/5) BclI 425 TGATCA T^GATCA BmyI 231 GTGCAC G[AGT]GC[ACT]^C BsaAI 228 CACGTG [CT]AC^GT[GA] BsaBI 84 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 800 GACGCC G[GA]^CG[CT]C BsiHKAI 231 GTGCAC G[AT]GC[AT]^C BsmAI 448 GTCTC GTCTC(1/5) BsmFI 275 GGGAC GGGAC(10/14) BsmI 845 GCATTC GAATGC(1/-1) Bsp1286I 231 GTGCAC G[AGT]GC[ACT]^C BspLU11I 77 ACATGT A^CATGT BstBI 581 TTCGAA TT^CGAA BstXI 789 CCAGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 344 GGATCT [GA]^GATC[CT] Bsu36I 241 CCTCAGG CC^T[ACGT]AGG CfoI 162 GCGC GCG^C Cfr13I 69 GGACC G^G[ACGT]CC DraI 492 TTTAAA TTT^AAA Eco81I 241 CCTCAGG CC^T[ACGT]AGG EcoT22I 566 ATGCAT ATGCA^T FbaI 425 TGATCA T^GATCA Fnu4HI 164 GCAGC GC^[ACGT]GC HgaI 800 GACGC GACGC(5/10) HhaI 162 GCGC GCG^C Hin1I 800 GACGCC G[GA]^CG[CT]C HinP1I 162 GCGC G^CGC HincII 618 GTCGAC GT[CT]^[GA]AC HindII 618 GTCGAC GT[CT]^[GA]AC ItaI 164 GCAGC GC^[ACGT]GC MaeII 229 ACGT A^CGT MamI 84 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 344 GGATCT [GA]^GATC[CT] MseI 493 TTAA T^TAA MslI 609 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 381 CATATG CA^TATG NsiI 566 ATGCAT ATGCA^T NspV 581 TTCGAA TT^CGAA PflFI 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 42 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 337 GAGTC GAGTC(4/5) PmaCI 228 CACGTG CAC^GTG PmlI 228 CACGTG CAC^GTG PshAI 621 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 618 GTCGAC G^TCGAC SapI 306 GCTCTTC GCTCTTC(1/4) Sau96I 69 GGACC G^G[ACGT]CC SfcI 64 CTACAG C^T[GA][CT]AG SfuI 581 TTCGAA TT^CGAA SphI 197 GCATGC GCATG^C SspI 520 AATATT AAT^ATT StuI 245 AGGCCT AGG^CCT Tru9I 493 TTAA T^TAA TseI 164 GCAGC G^C[AT]GC Tth111I 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 42 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 344 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbsI 37 GTCTTC GAAGAC(2/6) 679 GTCTTC BfaI 26 CTAG C^TAG 556 CTAG BpmI 695 CTCCAG CTGGAG(16/14) 206 CTGGAG BpuAI 37 GTCTTC GAAGAC(2/6) 679 GTCTTC BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG 284 CCCTGG EarI 187 CTCTTC CTCTTC(1/4) 307 CTCTTC HphI 354 GGTGA GGTGA(8/7) 113 TCACC Ksp632I 187 CTCTTC CTCTTC(1/4) 307 CTCTTC MaeI 26 CTAG C^TAG 556 CTAG MaeIII 112 GTCAC ^GT[ACGT]AC 442 GTGAC MwoI 53 GCATCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 559 GCCAAATATGC TaqI 582 TCGA T^CGA 619 TCGA TfiI 666 GATTC G^A[AT]TC 867 GAATC Tsp45I 112 GTCAC ^GT[GC]AC 442 GTGAC TspRI 18 CAGTG CA[GC]TG[ACGT][ACGT]^ 392 CACTG TthHB8I 582 TCGA T^CGA 619 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI AlwI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI SfaNI Tsp509I