(Circular) (Six-base) MAPSORT of: pFN21AE6013.seq Check: 6482 from: 1 to: 5647 pFN21AE6013 5647 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 2990 1937 Size: 687 366 4594 Fragments arranged by size: 4594 687 366 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3710 5094 5362 1632 Size: 2078 1384 268 1917 Fragments arranged by size: 2078 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2082 4546 5523 1203 Size: 879 2464 977 1327 Fragments arranged by size: 2464 1327 977 879 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaLI G'TGCA_C Cuts at: 2236 3704 4860 2236 Size: 1468 1156 3023 Fragments arranged by size: 3023 1468 1156 ApoI r'AATT_y Cuts at: 1493 2439 2956 3182 3334 5443 1493 Size: 946 517 226 152 2109 1697 Fragments arranged by size: 2109 1697 946 517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2171 2204 2171 Size: 33 5614 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2968 4423 618 Size: 325 217 627 1181 1455 1842 Fragments arranged by size: 1842 1455 1181 627 325 217 BanII G_rGCy'C Cuts at: 1391 2966 1391 Size: 1575 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2677 928 Size: 971 136 642 3898 Fragments arranged by size: 3898 971 642 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3838 3872 1903 Size: 34 50 34 1817 34 3678 Fragments arranged by size: 3678 1817 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2834 3539 4749 2834 Size: 705 1210 3732 Fragments arranged by size: 3732 1210 705 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 4765 BfrBI ATG'CAT Cuts at: 2573 2573 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2240 3708 4864 1790 Size: 450 1468 1156 2573 Fragments arranged by size: 2573 1468 1156 450 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2685 4307 1239 Size: 993 453 1622 2579 Fragments arranged by size: 2579 1622 993 453 Bpu10I CC'TnA_GC Cuts at: 2254 2254 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2235 3438 493 Size: 711 1031 1203 2702 Fragments arranged by size: 2702 1203 1031 711 BsaBI GATnn'nnATC Cuts at: 2093 5641 2093 Size: 3548 2099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2806 3454 3836 4471 Size: 53 83 186 936 1273 648 382 635 Cuts at: 4471 275 Size: 1451 Fragments arranged by size: 1451 1273 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2053 2276 4850 5346 2053 Size: 223 2574 496 2354 Fragments arranged by size: 2574 2354 496 223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5074 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2240 3708 3793 4864 2240 Size: 1468 85 1071 3023 Fragments arranged by size: 3023 1468 1071 85 BsmI GAATG_Cn' Cuts at: 2850 3158 3251 2850 Size: 308 93 5246 Fragments arranged by size: 5246 308 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2966 3708 3793 4864 1391 Size: 399 450 726 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 450 399 85 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3002 844 Size: 2158 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 2587 4451 2587 Size: 1864 3783 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2349 2977 3344 3729 3746 5198 5284 5296 Size: 377 628 367 385 17 1452 86 12 Cuts at: 5296 5642 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 628 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2247 2247 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5095 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2499 2951 2499 Size: 452 5195 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612 Size: 54 1425 50 275 18 1264 889 1629 Cuts at: 5612 8 Size: 43 Fragments arranged by size: 1629 1425 1264 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2318 3577 1353 Size: 845 120 1259 3423 Fragments arranged by size: 3423 1259 845 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2685 3710 4307 5094 5362 1239 Size: 393 600 453 1025 597 787 268 1524 Fragments arranged by size: 1524 1025 787 600 597 453 393 268 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2526 2526 Size: 5647 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 4288 4320 4362 Size: 32 135 32 868 32 1816 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 1816 868 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2625 2991 3237 669 Size: 988 281 687 366 246 3079 Fragments arranged by size: 3079 988 687 366 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 3605 3964 4123 518 Size: 648 1452 987 359 159 2042 Fragments arranged by size: 2042 1452 987 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3063 3740 4888 5133 1606 Size: 1457 677 1148 245 2120 Fragments arranged by size: 2120 1457 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5095 NdeI CA'TA_TG Cuts at: 387 2387 387 Size: 2000 3647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NsiI A_TGCA'T Cuts at: 2575 2575 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2086 2206 2577 2695 3005 4550 1132 Size: 97 857 120 371 118 310 1545 2229 Fragments arranged by size: 2229 1545 857 371 310 120 118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 2082 4546 2082 Size: 2464 3183 PflMI CCAn_nnn'nTGG Cuts at: 1108 2053 1108 Size: 945 4702 PfoI T'CCnGG_A Cuts at: 2907 2907 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PmlI CAC'GTG Cuts at: 2235 2235 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2630 1613 Size: 1017 4630 PsiI TTA'TAA Cuts at: 2936 3217 2936 Size: 281 5366 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2623 2989 1936 Size: 687 366 4594 Fragments arranged by size: 4594 687 366 SapI GCTCTTCn'nnn_ Cuts at: 2318 2318 Size: 5647 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2069 2995 4130 4811 5002 826 Size: 219 1024 926 1135 681 191 1471 Fragments arranged by size: 1471 1135 1024 926 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 2206 3005 1132 Size: 1074 799 3774 Fragments arranged by size: 3774 1074 799 SspI AAT'ATT Cuts at: 5 52 2527 3571 5 Size: 47 2475 1044 2081 Fragments arranged by size: 2475 2081 1044 47 StuI AGG'CCT Cuts at: 1517 2252 1517 Size: 735 4912 StyI C'CwwG_G Cuts at: 513 1065 3086 513 Size: 552 2021 3074 Fragments arranged by size: 3074 2021 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3674 3691 3844 4029 4474 325 Size: 1255 2094 17 153 185 445 1498 Fragments arranged by size: 2094 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2644 3893 5605 Size: 274 80 33 51 1443 667 1249 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1443 1249 667 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 5607 5639 2808 Size: 32 2767 32 2816 Fragments arranged by size: 2816 2767 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2222 1316 Size: 906 4741 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI