################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 211 GTAGAC GT^[AC][GT]AC AcsI 4 GAATTT [GA]^AATT[CT] AflIII 23 ACACGT A^C[GA][CT]GT ApoI 4 GAATTT [GA]^AATT[CT] BbsI 711 GAAGAC GAAGAC(2/6) BclI 149 TGATCA T^GATCA BfaI 416 CTAG C^TAG BglI 352 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 296 GTGCCC G[AGT]GC[ACT]^C BpuAI 711 GAAGAC GAAGAC(2/6) BsgI 570 CTGCAC GTGCAG(16/14) BsiYI 284 CCTATTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 284 CCTATTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 763 GGGAC GGGAC(10/14) Bsp1286I 296 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 398 TGTACA T^GTACA BsrBI 690 CCGCTC CCGCTC(-3/-3) BsrGI 398 TGTACA T^GTACA BsrI 853 CCAGT ACTGG(1/-1) BstXI 242 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 686 AGATCC [GA]^GATC[CT] CfoI 728 GCGC GCG^C Cfr13I 788 GGCCC G^G[ACGT]CC DsaI 356 CCATGG C^C[GA][CT]GG EaeI 350 TGGCCG [CT]^GGCC[GA] FbaI 149 TGATCA T^GATCA HhaI 728 GCGC GCG^C HinP1I 728 GCGC G^CGC MaeI 416 CTAG C^TAG MaeII 25 ACGT A^CGT MflI 686 AGATCC [GA]^GATC[CT] MspA1I 905 CAGCTG C[AC]G^C[GT]G MwoI 352 GCCGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 356 CCATGG C^CATGG NlaIV 596 GGCTCC GG[ACGT]^[ACGT]CC NspI 724 ACATGC [GA]CATG^[CT] PleI 765 GACTC GAGTC(4/5) PvuII 905 CAGCTG CAG^CTG Sau96I 788 GGCCC G^G[ACGT]CC SfiI 351 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 398 TGTACA T^GTACA SspI 506 AATATT AAT^ATT XhoII 686 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 320 GTAC GT^AC 399 GTAC AlwI 160 GATCC GGATC(4/5) 687 GATCC AseI 526 ATTAAT AT^TAAT 586 ATTAAT AsnI 526 ATTAAT AT^TAAT 586 ATTAAT BbvI 614 GCAGC GCAGC(8/12) 904 GCAGC BseRI 598 CTCCTC GAGGAG(10/8) 769 CTCCTC BssKI 348 CCTGG ^CC[ACGT]GG 474 CCTGG BstNI 348 CCTGG CC^[AT]GG 474 CCTGG EcoRII 348 CCTGG ^CC[AT]GG 474 CCTGG HphI 165 TCACC GGTGA(8/7) 735 TCACC MvaI 348 CCTGG CC^[AT]GG 474 CCTGG PshBI 526 ATTAAT AT^TAAT 586 ATTAAT PstI 73 CTGCAG CTGCA^G 301 CTGCAG RsaI 320 GTAC GT^AC 399 GTAC ScrFI 348 CCTGG CC^[ACGT]GG 474 CCTGG SfcI 73 CTGCAG C^T[GA][CT]AG 301 CTGCAG TseI 614 GCAGC G^C[AT]GC 904 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BsaJI BstF5I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII Sau3AI SfaNI StyI TfiI Tru9I Tsp45I Tsp509I TspRI