################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BcgI BclI BcnI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 225 GTCTAC GT^[AC][GT]AC AciI 369 CCGC CCGC(-3/-1) AflII 495 CTTAAG C^TTAAG Alw44I 73 GTGCAC G^TGCAC AocI 453 CCTGAGG CC^T[ACGT]AGG ApaLI 73 GTGCAC G^TGCAC BamHI 662 GGATCC G^GATCC BanII 175 GAGCCC G[GA]GC[CT]^C BbsI 676 GTCTTC GAAGAC(2/6) BbvCI 912 GCTGAGG CCTCAGC(-5/-2) BbvI 133 GCTGC GCAGC(8/12) BciVI 53 GTATCC GTATCC(6/5) BfaI 64 CTAG C^TAG BfrI 495 CTTAAG C^TTAAG BglI 559 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 676 GTCTTC GAAGAC(2/6) Bsp1407I 464 TGTACA T^GTACA BsrGI 464 TGTACA T^GTACA BstAPI 439 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 242 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 662 GGATCC [GA]^GATC[CT] Bsu36I 453 CCTGAGG CC^T[ACGT]AGG DsaI 356 CCATGG C^C[GA][CT]GG Eco81I 453 CCTGAGG CC^T[ACGT]AGG EcoT14I 356 CCATGG C^C[AT][AT]GG Fnu4HI 133 GCTGC GC^[ACGT]GC HincII 586 GTCAAC GT[CT]^[GA]AC HindII 586 GTCAAC GT[CT]^[GA]AC HphI 413 TCACC GGTGA(8/7) ItaI 133 GCTGC GC^[ACGT]GC MaeI 64 CTAG C^TAG MflI 662 GGATCC [GA]^GATC[CT] MseI 496 TTAA T^TAA NcoI 356 CCATGG C^CATGG NlaIV 662 GGATCC GG[ACGT]^[ACGT]CC SfcI 5 CTATAG C^T[GA][CT]AG SmlI 495 CTTAAG C^T[CT][GA]AG SspBI 464 TGTACA T^GTACA StyI 356 CCATGG C^C[AT][AT]GG TaqI 702 TCGA T^CGA Tru9I 496 TTAA T^TAA TseI 133 GCTGC G^C[AT]GC TthHB8I 702 TCGA T^CGA XhoII 662 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BseRI 490 CTCCTC GAGGAG(10/8) 456 GAGGAG BsmAI 904 GAGAC GTCTC(1/5) 167 GTCTC BsrI 480 ACTGG ACTGG(1/-1) 21 CCAGT MslI 327 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 733 CACATTGGTG MwoI 439 GCAGGCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 559 GCCCGTCTGGC PleI 704 GACTC GAGTC(4/5) 165 GAGTC TfiI 89 GATTC G^A[AT]TC 205 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI AlwI AspHI BalI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrDI BssKI BstF5I BstNI Cac8I DdeI DpnII EaeI EcoRII FokI HaeIII HinfI MboI MboII MluNI MnlI MscI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TspRI