Restriction map for
pFN21AE5974
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AlwI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BfaI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BsrI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcsI 886 GAATTT [GA]^AATT[CT]
AcyI 895 GACGCC G[GA]^CG[CT]C
AflIII 218 ACGTGT A^C[GA][CT]GT
Alw44I 324 GTGCAC G^TGCAC
AlwNI 70 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApaLI 324 GTGCAC G^TGCAC
ApoI 886 GAATTT [GA]^AATT[CT]
Asp700I 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 411 CCCGGG C^[CT]CG[GA]G
AviII 677 TGCGCA TGC^GCA
BbsI 748 GTCTTC GAAGAC(2/6)
BcgI 272 GCATCTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 470 TGATCA T^GATCA
BglI 346 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 209 AGATCT A^GATCT
BpuAI 748 GTCTTC GAAGAC(2/6)
BsaHI 895 GACGCC G[GA]^CG[CT]C
BsgI 564 CTGCAC GTGCAG(16/14)
BsiEI 360 CGACCG CG[GA][CT]^CG
BsoBI 411 CCCGGG C^[CT]CG[GA]G
Bsp1407I 830 TGTACA T^GTACA
BsrBI 628 CCGCTC CCGCTC(-3/-3)
BsrFI 694 ACCGGC [GA]^CCGG[CT]
BsrGI 830 TGTACA T^GTACA
BstAPI 69 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 741 GGTCACC G^GT[ACGT]ACC
BstPI 741 GGTCACC G^GT[ACGT]ACC
BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 209 AGATCT [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cfr10I 694 ACCGGC [GA]^CCGG[CT]
DraIII 327 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO65I 741 GGTCACC G^GT[ACGT]ACC
FauI 384 CCCGC CCCGC(4/6)
FbaI 470 TGATCA T^GATCA
FspI 677 TGCGCA TGC^GCA
Hin1I 895 GACGCC G[GA]^CG[CT]C
HinfI 796 GATTC G^A[ACGT]TC
MflI 209 AGATCT [GA]^GATC[CT]
NcoI 350 CCATGG C^CATGG
PflMI 426 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 427 CAGCTG CAG^CTG
SapI 78 GCTCTTC GCTCTTC(1/4)
SfiI 345 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 411 CCCGGG CCC^GGG
SmlI 553 CTCAAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
StuI 908 AGGCCT AGG^CCT
TfiI 796 GATTC G^A[AT]TC
Van91I 426 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 209 AGATCT [GA]^GATC[CT]
XmaCI 411 CCCGGG C^CCGGG
XmaI 411 CCCGGG C^CCGGG
XmnI 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanII 189 GAGCTC G[GA]GC[CT]^C
804 GAGCTC
BcnI 411 CCCGG CC^[GC]GG
412 CCGGG
BsaAI 217 TACGTG [CT]AC^GT[GA]
367 TACGTG
BseRI 85 CTCCTC GAGGAG(10/8)
256 CTCCTC
BspMI 74 ACCTGC ACCTGC(4/8)
718 ACCTGC
Cac8I 558 GCTGGC GC[ACGT]^[ACGT]GC
562 GCCTGC
DdeI 187 CTGAG C^T[ACGT]AG
874 CTGAG
DpnII 210 GATC ^GATC
471 GATC
EarI 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
Ksp632I 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
MboI 210 GATC ^GATC
471 GATC
MseI 854 TTAA T^TAA
890 TTAA
MspA1I 385 CCGCTG C[AC]G^C[GT]G
427 CAGCTG
MwoI 69 GCAGTACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCCTCCATGGC
NciI 411 CCCGG CC^[GC]GG
412 CCGGG
NdeII 210 GATC ^GATC
471 GATC
PstI 67 CTGCAG CTGCA^G
581 CTGCAG
SacI 189 GAGCTC GAGCT^C
804 GAGCTC
Sau3AI 210 GATC ^GATC
471 GATC
SfcI 67 CTGCAG C^T[GA][CT]AG
581 CTGCAG
Tru9I 854 TTAA T^TAA
890 TTAA
Tsp509I 852 AATT ^AATT
887 AATT
TspRI 579 CACTG CA[GC]TG[ACGT][ACGT]^
834 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI CfoI Cfr13I DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboII
MluNI MnlI MscI MslI MspI
MvaI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
XcmI