(Circular) (Six-base) MAPSORT of: pFN21AE5974.seq Check: 1413 from: 1 to: 5641 pFN21AE5974 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 3704 5088 5356 1632 Size: 667 1405 1384 268 1917 Fragments arranged by size: 1917 1405 1384 667 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2223 2938 4540 5517 1203 Size: 1020 715 1602 977 1327 Fragments arranged by size: 1602 1327 1020 977 715 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2080 3384 4956 2080 Size: 1304 1572 2765 Fragments arranged by size: 2765 1572 1304 ApaLI G'TGCA_C Cuts at: 2329 3698 4854 2329 Size: 1369 1156 3116 Fragments arranged by size: 3116 1369 1156 ApoI r'AATT_y Cuts at: 1493 2891 2950 3176 3328 5437 1493 Size: 1398 59 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2416 2966 1870 Size: 73 473 550 4545 Fragments arranged by size: 4545 550 473 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2198 2813 2960 1391 Size: 807 615 147 4072 Fragments arranged by size: 4072 807 615 147 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2746 928 Size: 971 847 3823 Fragments arranged by size: 3823 971 847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2266 2300 3832 3866 1903 Size: 34 329 34 1532 34 3678 Fragments arranged by size: 3678 1532 329 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 4714 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 4252 136 Size: 107 122 71 631 605 685 1895 1525 Fragments arranged by size: 1895 1525 685 631 605 122 107 71 BglII A'GATC_T Cuts at: 2214 5636 2214 Size: 3422 2219 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2333 3702 4858 1790 Size: 543 1369 1156 2573 Fragments arranged by size: 2573 1369 1156 543 BmgBI CAC'GTC Cuts at: 1318 2404 1318 Size: 1086 4555 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2231 2249 2270 2414 2479 2728 4301 Size: 934 58 18 21 144 65 249 1573 Cuts at: 4301 1239 Size: 2579 Fragments arranged by size: 2579 1573 934 249 144 65 58 21 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3766 4631 4929 2543 Size: 1223 865 298 3255 Fragments arranged by size: 3255 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 2374 3432 493 Size: 711 1020 150 1058 2702 Fragments arranged by size: 2702 1058 1020 711 150 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2901 3448 3830 4465 Size: 53 83 186 936 1368 547 382 635 Cuts at: 4465 275 Size: 1451 Fragments arranged by size: 1451 1368 936 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 4585 Fragments arranged by size: 4585 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2252 1186 Size: 895 171 4575 Fragments arranged by size: 4575 895 171 BseYI C'CCAG_C Cuts at: 4844 5340 4844 Size: 496 5145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 1163 Size: 573 818 4250 Fragments arranged by size: 4250 818 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3091 3852 4880 1540 Size: 275 186 367 723 761 1028 2301 Fragments arranged by size: 2301 1028 761 723 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2198 2333 2813 3702 3787 4858 2198 Size: 135 480 889 85 1071 2981 Fragments arranged by size: 2981 1071 889 480 135 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2333 2813 2960 3702 3787 4858 Size: 399 408 135 480 147 742 85 1071 Cuts at: 4858 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 480 408 399 147 135 85 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2732 2996 844 Size: 1244 644 264 3489 Fragments arranged by size: 3489 1244 644 264 BsrBI CCG'CTC Cuts at: 1394 1823 2635 3528 1394 Size: 429 812 893 3507 Fragments arranged by size: 3507 893 812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2699 4285 4472 1700 Size: 110 889 1586 187 2869 Fragments arranged by size: 2869 1586 889 187 110 BsrGI T'GTAC_A Cuts at: 96 2835 96 Size: 2739 2902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 794 Size: 1286 4355 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2746 1184 Size: 1562 4079 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2214 2971 3338 3723 3740 5192 5278 5290 Size: 242 757 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 757 385 367 346 242 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2879 2879 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2058 2355 2742 513 Size: 552 993 297 387 3412 Fragments arranged by size: 3412 993 552 387 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2583 3157 3951 3971 2583 Size: 574 794 20 4253 Fragments arranged by size: 4253 794 574 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2376 2640 Size: 54 1425 50 275 18 303 243 264 Cuts at: 2640 2694 3088 3977 5606 8 Size: 54 394 889 1629 43 Fragments arranged by size: 1629 1425 889 394 303 275 264 243 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2099 3571 1353 Size: 737 9 1472 3423 Fragments arranged by size: 3423 1472 737 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2196 2811 2196 Size: 615 5026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2231 2249 2270 2299 2414 2479 Size: 393 541 58 18 21 29 115 65 Cuts at: 2479 2728 3704 4301 5088 5356 1239 Size: 249 976 597 787 268 1524 Fragments arranged by size: 1524 976 787 597 541 393 268 249 115 65 58 29 21 18 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 5641 FspI TGC'GCA Cuts at: 2684 4147 2684 Size: 1463 4178 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2541 2838 4755 4939 1305 Size: 1236 297 1917 184 2007 Fragments arranged by size: 2007 1917 1236 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2378 2642 10 Size: 54 1425 343 303 243 264 3009 Fragments arranged by size: 3009 1425 343 303 264 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2471 2624 3599 3958 4117 518 Size: 648 879 426 153 975 359 159 2042 Fragments arranged by size: 2042 975 879 648 426 359 159 153 MspA1I CmG'CkG Cuts at: 1606 2392 2434 3057 3734 4882 5127 1606 Size: 786 42 623 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 623 245 42 NcoI C'CATG_G Cuts at: 513 1065 2355 513 Size: 552 1290 3799 Fragments arranged by size: 3799 1290 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 2066 1348 Size: 718 4923 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 1108 Size: 1329 4312 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2076 2590 2993 830 Size: 1246 514 403 3478 Fragments arranged by size: 3478 1246 514 403 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2434 2434 Size: 5641 SacI G_AGCT'C Cuts at: 2198 2813 2198 Size: 615 5026 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SapI GCTCTTCn'nnn_ Cuts at: 2090 2090 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2072 2586 2989 4124 4805 4996 826 Size: 219 1027 514 403 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 514 403 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2357 2357 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2418 2968 1872 Size: 546 550 4545 Fragments arranged by size: 4545 550 546 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3745 4646 4908 820 Size: 197 926 615 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 615 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2915 1517 Size: 1398 4243 StyI C'CwwG_G Cuts at: 513 1065 2025 2355 2916 3080 513 Size: 552 960 330 561 164 3074 Fragments arranged by size: 3074 960 561 552 330 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2835 3887 Size: 274 80 33 51 1443 204 654 1052 Cuts at: 3887 5599 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1052 654 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2560 2590 2206 Size: 354 30 5257 Fragments arranged by size: 5257 354 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2416 2966 1870 Size: 546 550 4545 Fragments arranged by size: 4545 550 546 XmnI GAAnn'nnTTC Cuts at: 2531 3770 2531 Size: 1239 4402 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI