################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 829 GTCTAC GT^[AC][GT]AC AfaI 177 GTAC GT^AC AflIII 718 ACGTGT A^C[GA][CT]GT AlwNI 814 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AviII 875 TGCGCA TGC^GCA BbrPI 717 CACGTG CAC^GTG BbvCI 686 CCTCAGC CCTCAGC(-5/-2) BcnI 158 CCCGG CC^[GC]GG BglII 299 AGATCT A^GATCT BsaAI 717 CACGTG [CT]AC^GT[GA] BseRI 903 GAGGAG GAGGAG(10/8) BsmAI 290 GTCTC GTCTC(1/5) BspMI 125 ACCTGC ACCTGC(4/8) BstAPI 424 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 876 GCGC GCG^C DraII 815 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 416 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 356 CCTACGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 815 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 318 CCTTGG C^C[AT][AT]GG FspI 875 TGCGCA TGC^GCA HhaI 876 GCGC GCG^C HinP1I 876 GCGC G^CGC HinfI 154 GACTC G^A[ACGT]TC MaeIII 536 GTGAC ^GT[ACGT]AC NciI 158 CCCGG CC^[GC]GG PleI 154 GACTC GAGTC(4/5) PmaCI 717 CACGTG CAC^GTG PmlI 717 CACGTG CAC^GTG PstI 295 CTGCAG CTGCA^G RsaI 177 GTAC GT^AC SapI 789 GAAGAGC GCTCTTC(1/4) StyI 318 CCTTGG C^C[AT][AT]GG Tsp45I 536 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 618 GATCC GGATC(4/5) 639 GATCC AspHI 673 GTGCTC G[AT]GC[AT]^C 838 GTGCTC BfaI 589 CTAG C^TAG 622 CTAG BmyI 673 GTGCTC G[AGT]GC[ACT]^C 838 GTGCTC BpmI 58 CTCCAG CTGGAG(16/14) 15 CTGGAG BsiHKAI 673 GTGCTC G[AT]GC[AT]^C 838 GTGCTC BsmI 191 GCATTC GAATGC(1/-1) 518 GCATTC Bsp1286I 673 GTGCTC G[AGT]GC[ACT]^C 838 GTGCTC BspHI 107 TCATGA T^CATGA 404 TCATGA BsrFI 380 GCCGGC [GA]^CCGG[CT] 694 GCCGGC BstF5I 231 CATCC GGATG(2/0) 762 CATCC BstYI 299 AGATCT [GA]^GATC[CT] 617 AGATCC Cfr10I 380 GCCGGC [GA]^CCGG[CT] 694 GCCGGC EarI 79 CTCTTC CTCTTC(1/4) 789 GAAGAG EcoRI 31 GAATTC G^AATTC 680 GAATTC FokI 231 CATCC GGATG(9/13) 762 CATCC Ksp632I 79 CTCTTC CTCTTC(1/4) 789 GAAGAG MaeI 589 CTAG C^TAG 622 CTAG MflI 299 AGATCT [GA]^GATC[CT] 617 AGATCC MwoI 424 GCAGAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 509 GCCCCAATCGC NaeI 380 GCCGGC GCC^GGC 694 GCCGGC NgoMIV 380 GCCGGC G^CCGGC 694 GCCGGC PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 813 CCAGGCCCTGG RcaI 107 TCATGA T^CATGA 404 TCATGA SfaNI 240 GATGC GCATC(5/9) 761 GCATC SfcI 295 CTGCAG C^T[GA][CT]AG 867 CTACAG Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 813 CCAGGCCCTGG XhoII 299 AGATCT [GA]^GATC[CT] 617 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AluI ApoI BbvI BsaJI BsgI BsiYI BslI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI TseI Tsp509I TspRI