(Circular) (Six-base) MAPSORT of: pFN21AE5961.seq Check: 224 from: 1 to: 5641 pFN21AE5961 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 2984 1937 Size: 898 149 4594 Fragments arranged by size: 4594 898 149 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2810 3704 5088 5356 1632 Size: 1178 894 1384 268 1917 Fragments arranged by size: 1917 1384 1178 894 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2723 4540 5517 1203 Size: 1520 1817 977 1327 Fragments arranged by size: 1817 1520 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2824 3384 4956 2824 Size: 560 1572 3509 Fragments arranged by size: 3509 1572 560 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2036 2481 2611 2685 2950 3176 3328 5437 Size: 543 445 130 74 265 226 152 2109 Cuts at: 5437 1493 Size: 1697 Fragments arranged by size: 2109 1697 543 445 265 226 152 130 74 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2165 2198 2165 Size: 33 5608 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2960 1391 Size: 1569 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BbvCI CC'TCA_GC Cuts at: 2692 2692 Size: 5641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2304 5636 2304 Size: 3332 2309 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 2533 1318 Size: 1215 4426 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2048 4301 1239 Size: 802 7 2253 2579 Fragments arranged by size: 2579 2253 802 7 Bpu10I CC'TnA_GC Cuts at: 2692 2692 Size: 5641 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2724 3432 493 Size: 711 1520 708 2702 Fragments arranged by size: 2702 1520 711 708 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2923 1186 Size: 1737 3904 BseYI C'CCAG_C Cuts at: 2465 4844 5340 2465 Size: 2379 496 2766 Fragments arranged by size: 2766 2379 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2448 2656 2681 2804 1163 Size: 573 712 208 25 123 4000 Fragments arranged by size: 4000 712 573 208 123 25 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 2847 3702 3787 4858 2682 Size: 165 855 85 1071 3465 Fragments arranged by size: 3465 1071 855 165 85 BsmI GAATG_Cn' Cuts at: 2196 2523 3152 3245 2196 Size: 327 629 93 4592 Fragments arranged by size: 4592 629 327 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 2847 2960 3702 3787 4858 1391 Size: 399 892 165 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 892 742 399 165 113 85 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 2112 2409 3530 5260 2112 Size: 297 1121 1730 2493 Fragments arranged by size: 2493 1730 1121 297 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2996 844 Size: 1295 857 3489 Fragments arranged by size: 3489 1295 857 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 2699 4285 4472 1700 Size: 110 575 314 1586 187 2869 Fragments arranged by size: 2869 1586 575 314 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 2435 794 Size: 1641 4000 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2304 2622 2971 3338 3723 3740 5192 5278 Size: 332 318 349 367 385 17 1452 86 Cuts at: 5278 5290 5636 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 349 346 332 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2835 3157 3951 3971 2835 Size: 322 794 20 4505 Fragments arranged by size: 4505 794 322 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2426 2426 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606 Size: 54 1425 50 275 18 1258 889 1629 Cuts at: 5606 8 Size: 43 Fragments arranged by size: 1629 1425 1258 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2789 3571 1353 Size: 737 699 782 3423 Fragments arranged by size: 3423 782 737 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2048 2810 3704 4301 5088 5356 Size: 393 409 7 762 894 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 894 787 762 597 409 393 268 7 EcoNI CCTnn'n_nnAGG Cuts at: 2365 2365 Size: 5641 EcoO109I rG'GnC_Cy Cuts at: 2821 2821 Size: 5641 EcoRI G'AATT_C Cuts at: 2036 2685 2950 3328 2036 Size: 649 265 378 4349 Fragments arranged by size: 4349 649 378 265 FspI TGC'GCA Cuts at: 2882 4147 2882 Size: 1265 4376 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2177 2780 3599 3958 4117 518 Size: 648 951 60 603 819 359 159 2042 Fragments arranged by size: 2042 951 819 648 603 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2228 2696 2788 3057 3734 4882 5127 1606 Size: 622 468 92 269 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 622 468 269 245 92 NaeI GCC'GGC Cuts at: 2387 2701 2387 Size: 314 5327 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2385 2699 2385 Size: 314 5327 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 2824 1108 Size: 1402 314 3925 Fragments arranged by size: 3925 1402 314 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2724 2724 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 2609 3211 2609 Size: 602 5039 PstI C_TGCA'G Cuts at: 830 2304 2993 830 Size: 1474 689 3478 Fragments arranged by size: 3478 1474 689 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2228 2696 2788 2228 Size: 468 92 5081 Fragments arranged by size: 5081 468 92 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SapI GCTCTTCn'nnn_ Cuts at: 2789 2789 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2300 2872 2989 4124 4805 4996 826 Size: 219 1255 572 117 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 681 572 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2323 3080 513 Size: 552 939 319 757 3074 Fragments arranged by size: 3074 939 757 552 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2280 3668 3685 3838 4023 4468 Size: 1255 611 89 1388 17 153 185 445 Cuts at: 4468 325 Size: 1498 Fragments arranged by size: 1498 1388 1255 611 445 185 153 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3887 5599 96 Size: 274 80 33 51 1443 1910 1712 138 Fragments arranged by size: 1910 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI