################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AgeI AhdI AlwI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BsrBI BsrDI BsrGI BsrI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 274 CGCG CG^CG AflII 924 CTTAAG C^TTAAG AflIII 723 ACATGT A^C[GA][CT]GT Alw44I 503 GTGCAC G^TGCAC AocI 417 CCTGAGG CC^T[ACGT]AGG ApaLI 503 GTGCAC G^TGCAC AspHI 503 GTGCAC G[AT]GC[AT]^C AspI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 36 CCTAGG C^CTAGG BbsI 574 GTCTTC GAAGAC(2/6) BcnI 144 CCGGG CC^[GC]GG BfaI 37 CTAG C^TAG BfrI 924 CTTAAG C^TTAAG BglII 293 AGATCT A^GATCT BlnI 36 CCTAGG C^CTAGG BpuAI 574 GTCTTC GAAGAC(2/6) BsaWI 253 ACCGGA [AT]^CCGG[AT] BsiHKAI 503 GTGCAC G[AT]GC[AT]^C BspDI 514 ATCGAT AT^CGAT BspLU11I 723 ACATGT A^CATGT BspMI 712 ACCTGC ACCTGC(4/8) BsrFI 152 ACCGGC [GA]^CCGG[CT] BssSI 508 CACGAG CACGAG(-5/-1) BstBI 577 TTCGAA TT^CGAA BstUI 274 CGCG CG^CG BstYI 293 AGATCT [GA]^GATC[CT] Bsu36I 417 CCTGAGG CC^T[ACGT]AGG Cfr10I 152 ACCGGC [GA]^CCGG[CT] ClaI 514 ATCGAT AT^CGAT DraII 533 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 565 GACACTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 344 CCATGG C^C[GA][CT]GG Eco81I 417 CCTGAGG CC^T[ACGT]AGG EcoO109I 533 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 386 GATATC GAT^ATC MaeI 37 CTAG C^TAG MaeII 203 ACGT A^CGT MflI 293 AGATCT [GA]^GATC[CT] MvnI 274 CGCG CG^CG NciI 144 CCGGG CC^[GC]GG NcoI 344 CCATGG C^CATGG NspV 577 TTCGAA TT^CGAA PflFI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 466 CTGCAG CTGCA^G SapI 716 GCTCTTC GCTCTTC(1/4) SbfI 465 CCTGCAGG CCTGCA^GG SfaNI 207 GATGC GCATC(5/9) SfuI 577 TTCGAA TT^CGAA SmlI 924 CTTAAG C^T[CT][GA]AG Sse8387I 465 CCTGCAGG CCTGCA^GG StuI 701 AGGCCT AGG^CCT Tsp509I 118 AATT ^AATT Tth111I 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 293 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 171 GTAC GT^AC 591 GTAC BsaI 774 GAGACC GGTCTC(1/5) 649 GGTCTC BsmAI 774 GAGAC GTCTC(1/5) 650 GTCTC CfoI 275 GCGC GCG^C 678 GCGC EarI 73 CTCTTC CTCTTC(1/4) 717 CTCTTC EcoT14I 36 CCTAGG C^C[AT][AT]GG 344 CCATGG FauI 272 CCCGC CCCGC(4/6) 692 CCCGC HhaI 275 GCGC GCG^C 678 GCGC HinP1I 275 GCGC G^CGC 678 GCGC Ksp632I 73 CTCTTC CTCTTC(1/4) 717 CTCTTC MspA1I 409 CAGCTG C[AC]G^C[GT]G 452 CAGCTG MwoI 156 GCTCCACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 340 GCAGCCATGGC NspI 674 ACATGC [GA]CATG^[CT] 723 ACATGT PleI 41 GACTC GAGTC(4/5) 740 GACTC PvuII 409 CAGCTG CAG^CTG 452 CAGCTG RsaI 171 GTAC GT^AC 591 GTAC StyI 36 CCTAGG C^C[AT][AT]GG 344 CCATGG TaqI 515 TCGA T^CGA 578 TCGA TfiI 129 GATTC G^A[AT]TC 138 GATTC TthHB8I 515 TCGA T^CGA 578 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspHI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI TspRI