################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AlwI 255 GGATC GGATC(4/5) BalI 182 TGGCCA TGG^CCA BanI 121 GGCACC G^G[CT][GA]CC BanII 163 GAGCCC G[GA]GC[CT]^C BciVI 454 GTATCC GTATCC(6/5) BglI 184 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 163 GAGCCC G[AGT]GC[ACT]^C BpmI 681 CTCCAG CTGGAG(16/14) Bsp1286I 163 GAGCCC G[AGT]GC[ACT]^C BspHI 159 TCATGA T^CATGA BspMI 792 GCAGGT ACCTGC(4/8) EaeI 182 TGGCCA [CT]^GGCC[GA] EcoNI 531 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 521 ATGCAT ATGCA^T MluNI 182 TGGCCA TGG^CCA MscI 182 TGGCCA TGG^CCA NcoI 344 CCATGG C^CATGG NsiI 521 ATGCAT ATGCA^T NspI 519 ACATGC [GA]CATG^[CT] PvuII 590 CAGCTG CAG^CTG RcaI 159 TCATGA T^CATGA StuI 293 AGGCCT AGG^CCT Tsp509I 927 AATT ^AATT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AciI 11 GCGG CCGC(-3/-1) 277 GCGG AvaI 322 CTCGAG C^[CT]CG[GA]G 410 CTCGAG BbvI 14 GCAGC GCAGC(8/12) 193 GCTGC BsaBI 148 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 449 GATTTGTATC BseRI 478 CTCCTC GAGGAG(10/8) 688 GAGGAG BsmAI 718 GTCTC GTCTC(1/5) 768 GTCTC BsoBI 322 CTCGAG C^[CT]CG[GA]G 410 CTCGAG BsrDI 675 CATTGC GCAATG(2/0) 116 GCAATG BsrI 37 ACTGG ACTGG(1/-1) 68 ACTGG Cfr13I 213 GGCCC G^G[ACGT]CC 695 GGGCC DdeI 382 CTTAG C^T[ACGT]AG 670 CTGAG DpnII 148 GATC ^GATC 256 GATC DsaI 230 CCACGG C^C[GA][CT]GG 344 CCATGG EcoT14I 344 CCATGG C^C[AT][AT]GG 698 CCAAGG MaeIII 500 GTCAC ^GT[ACGT]AC 751 GTCAC MamI 148 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 449 GATTTGTATC MboI 148 GATC ^GATC 256 GATC MseI 140 TTAA T^TAA 548 TTAA MslI 502 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 535 CACCAGGATG MspA1I 9 CAGCGG C[AC]G^C[GT]G 590 CAGCTG MwoI 184 GCCATGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 228 GCCCACGGTGC NdeII 148 GATC ^GATC 256 GATC PaeR7I 322 CTCGAG C^TCGAG 410 CTCGAG PleI 242 GAGTC GAGTC(4/5) 325 GAGTC Sau3AI 148 GATC ^GATC 256 GATC Sau96I 213 GGCCC G^G[ACGT]CC 695 GGGCC StyI 344 CCATGG C^C[AT][AT]GG 698 CCAAGG TaqI 323 TCGA T^CGA 411 TCGA Tru9I 140 TTAA T^TAA 548 TTAA TseI 14 GCAGC G^C[AT]GC 193 GCTGC Tsp45I 500 GTCAC ^GT[GC]AC 751 GTCAC TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^ 755 CAGTG TthHB8I 323 TCGA T^CGA 411 TCGA XhoI 322 CTCGAG C^TCGAG 410 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BsmI BssKI BstF5I BstNI BstXI Cac8I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboII MnlI MvaI NlaIII NlaIV RsaI ScrFI SmlI TfiI