################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BbvI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MroI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 202 GTCGAC GT^[AC][GT]AC AccII 429 CGCG CG^CG AcsI 515 AAATTT [GA]^AATT[CT] AlwI 678 GATCC GGATC(4/5) ApoI 515 AAATTT [GA]^AATT[CT] Asp700I 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 228 GGTGCC G^G[CT][GA]CC BbsI 103 GTCTTC GAAGAC(2/6) BcgI 419 GCATCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 233 CCCGG CC^[GC]GG BpuAI 103 GTCTTC GAAGAC(2/6) BsaAI 364 TACGTG [CT]AC^GT[GA] BsiEI 197 CGGTCG CG[GA][CT]^CG BsmAI 332 GTCTC GTCTC(1/5) BspHI 401 TCATGA T^CATGA BspMI 715 ACCTGC ACCTGC(4/8) BstUI 429 CGCG CG^CG BstYI 677 AGATCC [GA]^GATC[CT] EcoNI 497 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 280 GCGGG CCCGC(4/6) Fnu4HI 284 GCCGC GC^[ACGT]GC HincII 202 GTCGAC GT[CT]^[GA]AC HindII 202 GTCGAC GT[CT]^[GA]AC ItaI 284 GCCGC GC^[ACGT]GC MfeI 635 CAATTG C^AATTG MflI 677 AGATCC [GA]^GATC[CT] MseI 839 TTAA T^TAA MunI 635 CAATTG C^AATTG MvnI 429 CGCG CG^CG MwoI 231 GCCCCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 233 CCCGG CC^[GC]GG PpuMI 621 GGGACCC [GA]G^G[AT]CC[CT] PshAI 623 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 401 TCATGA T^CATGA SalI 202 GTCGAC G^TCGAC SapI 84 GCTCTTC GCTCTTC(1/4) TaqI 203 TCGA T^CGA Tru9I 839 TTAA T^TAA TthHB8I 203 TCGA T^CGA XhoII 677 AGATCC [GA]^GATC[CT] XmnI 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 174 GTAC GT^AC 363 GTAC BalI 368 TGGCCA TGG^CCA 395 TGGCCA BanII 82 GGGCTC G[GA]GC[CT]^C 136 GGGCTC BseRI 73 CTCCTC GAGGAG(10/8) 334 CTCCTC DdeI 871 CTTAG C^T[ACGT]AG 879 CTCAG DpnII 358 GATC ^GATC 678 GATC DraII 505 AGGCCCC [GA]G^G[ACGT]CC[CT] 621 GGGACCC EarI 85 CTCTTC CTCTTC(1/4) 900 GAAGAG EcoO109I 505 AGGCCCC [GA]G^G[ACGT]CC[CT] 621 GGGACCC EcoT14I 58 CCAAGG C^C[AT][AT]GG 797 CCAAGG HapII 234 CCGG C^CGG 599 CCGG HpaII 234 CCGG C^CGG 599 CCGG Ksp632I 85 CTCTTC CTCTTC(1/4) 900 GAAGAG MaeII 101 ACGT A^CGT 365 ACGT MboI 358 GATC ^GATC 678 GATC MluNI 368 TGGCCA TGG^CCA 395 TGGCCA MscI 368 TGGCCA TGG^CCA 395 TGGCCA MspI 234 CCGG C^CGG 599 CCGG NdeII 358 GATC ^GATC 678 GATC RsaI 174 GTAC GT^AC 363 GTAC Sau3AI 358 GATC ^GATC 678 GATC SfcI 501 CTGTAG C^T[GA][CT]AG 864 CTGTAG StyI 58 CCAAGG C^C[AT][AT]GG 797 CCAAGG TfiI 63 GATTC G^A[AT]TC 468 GATTC Tsp45I 322 GTCAC ^GT[GC]AC 433 GTGAC TspRI 146 CACTG CA[GC]TG[ACGT][ACGT]^ 447 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AvaII BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I EaeI EcoRII FokI HaeIII HinfI HphI MaeIII MboII MnlI MslI MvaI NlaIII NlaIV PleI Sau96I ScrFI SfaNI Tsp509I