################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AvaII AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HindIII HpaI HpaII KasI KspI MaeI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 208 GACGTC GACGT^C Acc65I 754 GGTACC G^GTACC AcsI 905 AAATTC [GA]^AATT[CT] AcyI 208 GACGTC G[GA]^CG[CT]C AocI 870 CCTGAGG CC^T[ACGT]AGG ApoI 905 AAATTC [GA]^AATT[CT] Asp718I 754 GGTACC G^GTACC BalI 668 TGGCCA TGG^CCA BamHI 680 GGATCC G^GATCC BanI 754 GGTACC G^G[CT][GA]CC BbsI 795 GTCTTC GAAGAC(2/6) BglI 346 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 68 GGGCAC G[AGT]GC[ACT]^C BpmI 256 CTGGAG CTGGAG(16/14) BpuAI 795 GTCTTC GAAGAC(2/6) BsaHI 208 GACGTC G[GA]^CG[CT]C BsaI 454 GGTCTC GGTCTC(1/5) BseRI 545 CTCCTC GAGGAG(10/8) BsgI 717 CTGCAC GTGCAG(16/14) BsmFI 232 GTCCC GGGAC(10/14) BsmI 46 GCATTC GAATGC(1/-1) Bsp1286I 68 GGGCAC G[AGT]GC[ACT]^C BspMI 715 ACCTGC ACCTGC(4/8) BsrI 512 CCAGT ACTGG(1/-1) BssKI 696 CCAGG ^CC[ACGT]GG BstEII 24 GGTGACC G^GT[ACGT]ACC BstNI 696 CCAGG CC^[AT]GG BstPI 24 GGTGACC G^GT[ACGT]ACC BstYI 680 GGATCC [GA]^GATC[CT] Bsu36I 870 CCTGAGG CC^T[ACGT]AGG DraII 893 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 284 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 629 CCATGG C^C[GA][CT]GG EaeI 668 TGGCCA [CT]^GGCC[GA] Eco81I 870 CCTGAGG CC^T[ACGT]AGG EcoO109I 893 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 24 GGTGACC G^GT[ACGT]ACC EcoRII 696 CCAGG ^CC[AT]GG FauI 419 CCCGC CCCGC(4/6) Hin1I 208 GACGTC G[GA]^CG[CT]C HincII 558 GTTGAC GT[CT]^[GA]AC HindII 558 GTTGAC GT[CT]^[GA]AC KpnI 754 GGTACC GGTAC^C MflI 680 GGATCC [GA]^GATC[CT] MluNI 668 TGGCCA TGG^CCA MscI 668 TGGCCA TGG^CCA MseI 553 TTAA T^TAA MvaI 696 CCAGG CC^[AT]GG NcoI 629 CCATGG C^CATGG PflMI 237 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScrFI 696 CCAGG CC^[ACGT]GG SfaNI 240 GATGC GCATC(5/9) SfcI 864 CTACAG C^T[GA][CT]AG SfiI 345 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 329 TCGA T^CGA Tru9I 553 TTAA T^TAA Tsp509I 906 AATT ^AATT TthHB8I 329 TCGA T^CGA Van91I 237 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 680 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AfaI 755 GTAC GT^AC 771 GTAC AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 382 CAGCCCCTG BsmAI 455 GTCTC GTCTC(1/5) 481 GTCTC EarI 79 CTCTTC CTCTTC(1/4) 745 CTCTTC Ksp632I 79 CTCTTC CTCTTC(1/4) 745 CTCTTC MaeII 209 ACGT A^CGT 446 ACGT PleI 189 GAGTC GAGTC(4/5) 426 GAGTC RsaI 755 GTAC GT^AC 771 GTAC TfiI 807 GAATC G^A[AT]TC 852 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsiYI BslI BstF5I Cac8I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SmlI StyI TseI Tsp45I TspRI