(Linear) (Six-base) MAPSORT of: KIEE5745.seq Check: 3862 from: 1 to: 1920 KIEE5745.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 790 1920 Size: 790 1130 Acc65I G'GTAC_C Cuts at: 0 431 1920 Size: 431 1489 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1920 Size: 295 1625 AflIII A'CryG_T Cuts at: 0 15 218 1360 1920 Size: 15 203 1142 560 Fragments arranged by size: 1142 560 203 15 AleI CACnn'nnGTG Cuts at: 0 328 1920 Size: 328 1592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1774 1920 Size: 1774 146 AlwNI CAG_nnn'CTG Cuts at: 0 447 1920 Size: 447 1473 ApaI G_GGCC'C Cuts at: 0 661 1122 1920 Size: 661 461 798 Fragments arranged by size: 798 661 461 ApaLI G'TGCA_C Cuts at: 0 623 1920 Size: 623 1297 ApoI r'AATT_y Cuts at: 0 1204 1420 1504 1920 Size: 1204 216 84 416 Fragments arranged by size: 1204 416 216 84 AvaI C'yCGr_G Cuts at: 0 488 1243 1920 Size: 488 755 677 Fragments arranged by size: 755 677 488 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 423 456 1920 Size: 423 33 1464 Fragments arranged by size: 1464 423 33 BamHI G'GATC_C Cuts at: 0 1268 1920 Size: 1268 652 BanI G'GyrC_C Cuts at: 0 431 1255 1920 Size: 431 824 665 Fragments arranged by size: 824 665 431 BanII G_rGCy'C Cuts at: 0 487 661 1031 1122 1400 1920 Size: 487 174 370 91 278 520 Fragments arranged by size: 520 487 370 278 174 91 BbsI GAAGACnn'nnnn_ Cuts at: 0 904 1920 Size: 904 1016 BfrBI ATG'CAT Cuts at: 0 1815 1920 Size: 1815 105 BlpI GC'TnA_GC Cuts at: 0 811 1920 Size: 811 1109 Bme1580I G_kGCm'C Cuts at: 0 627 661 707 1122 1258 1920 Size: 627 34 46 415 136 662 Fragments arranged by size: 662 627 415 136 46 34 BmgBI CAC'GTC Cuts at: 0 337 375 1920 Size: 337 38 1545 Fragments arranged by size: 1545 337 38 BmtI G_CTAG'C Cuts at: 0 196 1920 Size: 196 1724 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 501 1920 Size: 501 1419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 526 553 610 787 805 1045 1920 Size: 526 27 57 177 18 240 875 Fragments arranged by size: 875 526 240 177 57 27 18 Bpu10I CC'TnA_GC Cuts at: 0 1836 1920 Size: 1836 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 703 969 1920 Size: 100 603 266 951 Fragments arranged by size: 951 603 266 100 BsaI GGTCTCn'nnnn_ Cuts at: 0 1774 1920 Size: 1774 146 BsaAI yAC'GTr Cuts at: 0 129 219 246 1920 Size: 129 90 27 1674 Fragments arranged by size: 1674 129 90 27 BsaBI GATnn'nnATC Cuts at: 0 1273 1920 Size: 1273 647 BsaHI Gr'CG_yC Cuts at: 0 438 752 1920 Size: 438 314 1168 Fragments arranged by size: 1168 438 314 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 617 647 778 808 1747 1777 1920 Size: 617 30 131 30 939 30 143 Fragments arranged by size: 939 617 143 131 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 190 386 538 562 607 1755 1920 Size: 190 196 152 24 45 1148 165 Fragments arranged by size: 1148 196 190 165 152 45 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 1066 1129 1207 1252 1636 1920 Size: 622 444 63 78 45 384 284 Fragments arranged by size: 622 444 384 284 78 63 45 BsiEI CG_ry'CG Cuts at: 0 141 1920 Size: 141 1779 BsiHKAI G_wGCw'C Cuts at: 0 124 251 487 627 812 1031 1920 Size: 124 127 236 140 185 219 889 Fragments arranged by size: 889 236 219 185 140 127 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 172 327 1920 Size: 172 155 1593 Fragments arranged by size: 1593 172 155 Bsp1286I G_dGCh'C Cuts at: 0 124 251 487 627 661 707 812 1031 Size: 124 127 236 140 34 46 105 219 Cuts at: 1031 1122 1258 1400 1920 Size: 91 136 142 520 Fragments arranged by size: 520 236 219 142 140 136 127 124 105 91 46 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 790 1920 Size: 790 1130 BsrBI CCG'CTC Cuts at: 0 978 1920 Size: 978 942 BsrFI r'CCGG_y Cuts at: 0 327 434 735 1920 Size: 327 107 301 1185 Fragments arranged by size: 1185 327 301 107 BssHII G'CGCG_C Cuts at: 0 390 1920 Size: 390 1530 BstAPI GCAn_nnn'nTGC Cuts at: 0 782 1230 1920 Size: 782 448 690 Fragments arranged by size: 782 690 448 BstYI r'GATC_y Cuts at: 0 210 1268 1920 Size: 210 1058 652 Fragments arranged by size: 1058 652 210 Bsu36I CC'TnA_GG Cuts at: 0 398 1637 1920 Size: 398 1239 283 Fragments arranged by size: 1239 398 283 BtgI C'CryG_G Cuts at: 0 197 356 740 935 1176 1733 1920 Size: 197 159 384 195 241 557 187 Fragments arranged by size: 557 384 241 197 195 187 159 BtsI GCAGTG_nn' Cuts at: 0 1437 1811 1920 Size: 1437 374 109 Fragments arranged by size: 1437 374 109 DraIII CAC_nnn'GTG Cuts at: 0 1152 1230 1920 Size: 1152 78 690 Fragments arranged by size: 1152 690 78 DrdI GACnn_nn'nnGTC Cuts at: 0 351 1920 Size: 351 1569 EaeI y'GGCC_r Cuts at: 0 138 452 599 737 932 1499 1677 1920 Size: 138 314 147 138 195 567 178 243 Fragments arranged by size: 567 314 243 195 178 147 138 138 EagI C'GGCC_G Cuts at: 0 138 1920 Size: 138 1782 EarI CTCTTCn'nnn_ Cuts at: 0 475 1096 1920 Size: 475 621 824 Fragments arranged by size: 824 621 475 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 357 1920 Size: 357 1563 EcoICRI GAG'CTC Cuts at: 0 485 1029 1920 Size: 485 544 891 Fragments arranged by size: 891 544 485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 526 553 610 787 805 1045 1920 Size: 295 231 27 57 177 18 240 875 Fragments arranged by size: 875 295 240 231 177 57 27 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 1920 Size: 235 1685 EcoO109I rG'GnC_Cy Cuts at: 0 270 528 1004 1119 1407 1521 1920 Size: 270 258 476 115 288 114 399 Fragments arranged by size: 476 399 288 270 258 115 114 EcoRI G'AATT_C Cuts at: 0 1420 1920 Size: 1420 500 FspI TGC'GCA Cuts at: 0 766 1920 Size: 766 1154 HaeII r_GCGC'y Cuts at: 0 447 1920 Size: 447 1473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1747 1779 1920 Size: 1747 32 141 Fragments arranged by size: 1747 141 32 KpnI G_GTAC'C Cuts at: 0 435 1920 Size: 435 1485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 997 1920 Size: 997 923 MscI TGG'CCA Cuts at: 0 454 601 1501 1920 Size: 454 147 900 419 Fragments arranged by size: 900 454 419 147 MslI CAynn'nnrTG Cuts at: 0 328 1492 1920 Size: 328 1164 428 Fragments arranged by size: 1164 428 328 MspA1I CmG'CkG Cuts at: 0 513 1178 1344 1920 Size: 513 665 166 576 Fragments arranged by size: 665 576 513 166 NaeI GCC'GGC Cuts at: 0 737 1920 Size: 737 1183 NcoI C'CATG_G Cuts at: 0 197 356 740 1733 1920 Size: 197 159 384 993 187 Fragments arranged by size: 993 384 197 187 159 NgoMIV G'CCGG_C Cuts at: 0 735 1920 Size: 735 1185 NheI G'CTAG_C Cuts at: 0 192 1920 Size: 192 1728 NotI GC'GGCC_GC Cuts at: 0 138 1920 Size: 138 1782 NsiI A_TGCA'T Cuts at: 0 1817 1920 Size: 1817 103 NspI r_CATG'y Cuts at: 0 19 974 1364 1761 1920 Size: 19 955 390 397 159 Fragments arranged by size: 955 397 390 159 19 PciI A'CATG_T Cuts at: 0 15 1360 1920 Size: 15 1345 560 Fragments arranged by size: 1345 560 15 PfoI T'CCnGG_A Cuts at: 0 206 1108 1920 Size: 206 902 812 Fragments arranged by size: 902 812 206 PmlI CAC'GTG Cuts at: 0 129 1920 Size: 129 1791 PpuMI rG'GwC_Cy Cuts at: 0 270 1407 1920 Size: 270 1137 513 Fragments arranged by size: 1137 513 270 PspOMI G'GGCC_C Cuts at: 0 657 1118 1920 Size: 657 461 802 Fragments arranged by size: 802 657 461 PspXI vC'TCGA_Gb Cuts at: 0 1243 1920 Size: 1243 677 PstI C_TGCA'G Cuts at: 0 1753 1920 Size: 1753 167 PvuII CAG'CTG Cuts at: 0 1344 1920 Size: 1344 576 RsrII CG'GwC_CG Cuts at: 0 303 1920 Size: 303 1617 SacI G_AGCT'C Cuts at: 0 487 1031 1920 Size: 487 544 889 Fragments arranged by size: 889 544 487 SacII CC_GC'GG Cuts at: 0 1179 1920 Size: 1179 741 SapI GCTCTTCn'nnn_ Cuts at: 0 475 1920 Size: 475 1445 SexAI A'CCwGG_T Cuts at: 0 1721 1920 Size: 1721 199 SfcI C'TryA_G Cuts at: 0 1626 1749 1920 Size: 1626 123 171 Fragments arranged by size: 1626 171 123 SgrAI Cr'CCGG_yG Cuts at: 0 327 1920 Size: 327 1593 SmaI CCC'GGG Cuts at: 0 490 1920 Size: 490 1430 SmlI C'TyrA_G Cuts at: 0 115 718 948 1243 1920 Size: 115 603 230 295 677 Fragments arranged by size: 677 603 295 230 115 SphI G_CATG'C Cuts at: 0 974 1920 Size: 974 946 StyI C'CwwG_G Cuts at: 0 197 356 740 1733 1920 Size: 197 159 384 993 187 Fragments arranged by size: 993 384 197 187 159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 617 649 1253 1285 1920 Size: 617 32 604 32 635 Fragments arranged by size: 635 617 604 32 32 Tth111I GACn'n_nGTC Cuts at: 0 376 1920 Size: 376 1544 XhoI C'TCGA_G Cuts at: 0 1243 1920 Size: 1243 677 XmaI C'CCGG_G Cuts at: 0 488 1920 Size: 488 1432 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NotI NsiI NspI PciI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII SapI SexAI SfcI SgrAI SmaI SmlI SphI StyI TstI Tth111I XhoI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BbeI BbvCI BcgI BciVI BclI BglI BglII BmrI BsaWI BseYI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NdeI NruI PacI PasI PflMI PmeI PpiI PshAI PsiI PsrI PvuI SalI SanDI SbfI ScaI SfiI SfoI SgfI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI XbaI XcmI XmnI ZraI