################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EarI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI Ksp632I MaeI MaeIII MamI MboII MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 933 GGTACC G^GTACC AflIII 626 ACATGT A^C[GA][CT]GT AhdI 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 662 AGCGCT AGC^GCT Asp718I 933 GGTACC G^GTACC AspEI 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 753 GGATCC G^GATCC BglII 455 AGATCT A^GATCT BsgI 1199 GTGCAG GTGCAG(16/14) BspLU11I 626 ACATGT A^CATGT BsrBI 914 CCGCTC CCGCTC(-3/-3) BsrI 599 ACTGG ACTGG(1/-1) BssSI 166 CACGAG CACGAG(-5/-1) DrdI 547 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 662 AGCGCT AGC^GCT EcoNI 1021 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 410 CCATGG C^C[AT][AT]GG FseI 848 GGCCGGCC GGCCGG^CC KpnI 933 GGTACC GGTAC^C NcoI 410 CCATGG C^CATGG NspI 626 ACATGT [GA]CATG^[CT] PshAI 338 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 421 CTGCAG CTGCA^G SfiI 1048 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1293 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1177 CTCAAG C^T[CT][GA]AG StyI 410 CCATGG C^C[AT][AT]GG TaqI 632 TCGA T^CGA TthHB8I 632 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 393 GTAC GT^AC 934 GTAC AocI 1025 CCTCAGG CC^T[ACGT]AGG 1439 CCTCAGG BseRI 211 GAGGAG GAGGAG(10/8) 214 GAGGAG BstAPI 11 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 675 GCAGCCCCTGC Bsu36I 1025 CCTCAGG CC^T[ACGT]AGG 1439 CCTCAGG Eco81I 1025 CCTCAGG CC^T[ACGT]AGG 1439 CCTCAGG MslI 78 CATAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 405 CATCACCATG NotI 271 GCGGCCGC GC^GGCCGC 473 GCGGCCGC PflMI 1251 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1353 CCAGGTCTTGG PpuMI 1335 GGGTCCT [GA]G^G[AT]CC[CT] 1444 GGGTCCC PvuII 12 CAGCTG CAG^CTG 925 CAGCTG RsaI 393 GTAC GT^AC 934 GTAC SacI 42 GAGCTC GAGCT^C 112 GAGCTC SfcI 421 CTGCAG C^T[GA][CT]AG 1244 CTATAG StuI 1056 AGGCCT AGG^CCT 1231 AGGCCT Van91I 1251 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1353 CCAGGTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MboI MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI SacII Sau3AI Sau96I ScrFI SfaNI SfoI SmaI TseI XhoII XmaCI XmaI