(Linear) (Six-base) MAPSORT of: KIBB9597.seq Check: 6395 from: 1 to: 1491 KIBB9597.orf With 182 enzymes: SgfI * December 5, 2014 12:54 .. Acc65I G'GTAC_C Cuts at: 0 933 1491 Size: 933 558 AfeI AGC'GCT Cuts at: 0 664 1491 Size: 664 827 AflIII A'CryG_T Cuts at: 0 626 1491 Size: 626 865 AhdI GACnn_n'nnGTC Cuts at: 0 615 1491 Size: 615 876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 664 1491 Size: 664 827 AlwNI CAG_nnn'CTG Cuts at: 0 17 681 1015 1491 Size: 17 664 334 476 Fragments arranged by size: 664 476 334 17 ApaI G_GGCC'C Cuts at: 0 134 329 1039 1045 1051 1491 Size: 134 195 710 6 6 440 Fragments arranged by size: 710 440 195 134 6 6 AvaI C'yCGr_G Cuts at: 0 143 260 288 328 582 1032 1038 1044 Size: 143 117 28 40 254 450 6 6 Cuts at: 1044 1287 1420 1491 Size: 243 133 71 Fragments arranged by size: 450 254 243 143 133 117 71 40 28 6 6 BamHI G'GATC_C Cuts at: 0 753 1491 Size: 753 738 BanI G'GyrC_C Cuts at: 0 298 790 933 1135 1213 1291 1491 Size: 298 492 143 202 78 78 200 Fragments arranged by size: 492 298 202 200 143 78 78 BanII G_rGCy'C Cuts at: 0 46 116 134 245 289 329 370 430 Size: 46 70 18 111 44 40 41 60 Cuts at: 430 1039 1045 1051 1306 1491 Size: 609 6 6 255 185 Fragments arranged by size: 609 255 185 111 70 60 46 44 41 40 18 6 6 BbeI G_GCGC'C Cuts at: 0 302 794 1217 1295 1491 Size: 302 492 423 78 196 Fragments arranged by size: 492 423 302 196 78 BglI GCCn_nnn'nGGC Cuts at: 0 307 745 1055 1268 1478 1491 Size: 307 438 310 213 210 13 Fragments arranged by size: 438 310 307 213 210 13 BglII A'GATC_T Cuts at: 0 455 1491 Size: 455 1036 Bme1580I G_kGCm'C Cuts at: 0 134 329 1039 1045 1051 1491 Size: 134 195 710 6 6 440 Fragments arranged by size: 710 440 195 134 6 6 BmgBI CAC'GTC Cuts at: 0 84 150 1491 Size: 84 66 1341 Fragments arranged by size: 1341 84 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1162 1491 Size: 1162 329 BsaHI Gr'CG_yC Cuts at: 0 256 299 654 791 1111 1214 1292 1491 Size: 256 43 355 137 320 103 78 199 Fragments arranged by size: 355 320 256 199 137 103 78 43 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 226 229 1491 Size: 226 3 1262 Fragments arranged by size: 1262 226 3 BseYI C'CCAG_C Cuts at: 0 117 1008 1083 1491 Size: 117 891 75 408 Fragments arranged by size: 891 408 117 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1220 1491 Size: 1220 271 BsiEI CG_ry'CG Cuts at: 0 53 275 477 623 1491 Size: 53 222 202 146 868 Fragments arranged by size: 868 222 202 146 53 BsiHKAI G_wGCw'C Cuts at: 0 46 116 155 1491 Size: 46 70 39 1336 Fragments arranged by size: 1336 70 46 39 Bsp1286I G_dGCh'C Cuts at: 0 46 116 134 155 245 289 329 370 Size: 46 70 18 21 90 44 40 41 Cuts at: 370 430 1039 1045 1051 1306 1491 Size: 60 609 6 6 255 185 Fragments arranged by size: 609 255 185 90 70 60 46 44 41 40 21 18 6 6 BsrBI CCG'CTC Cuts at: 0 916 1491 Size: 916 575 BsrFI r'CCGG_y Cuts at: 0 849 1132 1294 1468 1491 Size: 849 283 162 174 23 Fragments arranged by size: 849 283 174 162 23 BssSI C'ACGA_G Cuts at: 0 166 1491 Size: 166 1325 BstAPI GCAn_nnn'nTGC Cuts at: 0 17 681 1491 Size: 17 664 810 Fragments arranged by size: 810 664 17 BstYI r'GATC_y Cuts at: 0 455 560 753 1491 Size: 455 105 193 738 Fragments arranged by size: 738 455 193 105 Bsu36I CC'TnA_GG Cuts at: 0 1026 1440 1491 Size: 1026 414 51 Fragments arranged by size: 1026 414 51 BtgI C'CryG_G Cuts at: 0 159 176 302 312 410 740 899 1139 Size: 159 17 126 10 98 330 159 240 Cuts at: 1139 1263 1331 1491 Size: 124 68 160 Fragments arranged by size: 330 240 160 159 159 126 124 98 68 17 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 381 1491 Size: 381 1110 DrdI GACnn_nn'nnGTC Cuts at: 0 553 1491 Size: 553 938 EaeI y'GGCC_r Cuts at: 0 50 162 272 413 474 620 1361 1398 Size: 50 112 110 141 61 146 741 37 Cuts at: 1398 1491 Size: 93 Fragments arranged by size: 741 146 141 112 110 93 61 50 37 EagI C'GGCC_G Cuts at: 0 50 272 474 620 1491 Size: 50 222 202 146 871 Fragments arranged by size: 871 222 202 146 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 514 1491 Size: 514 977 EcoICRI GAG'CTC Cuts at: 0 44 114 1491 Size: 44 70 1377 Fragments arranged by size: 1377 70 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 1025 1491 Size: 1025 466 EcoO109I rG'GnC_Cy Cuts at: 0 131 325 1048 1173 1336 1445 1491 Size: 131 194 723 125 163 109 46 Fragments arranged by size: 723 194 163 131 125 109 46 FseI GG_CCGG'CC Cuts at: 0 853 1491 Size: 853 638 HaeII r_GCGC'y Cuts at: 0 302 666 764 794 1217 1295 1491 Size: 302 364 98 30 423 78 196 Fragments arranged by size: 423 364 302 196 98 78 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 202 234 601 633 1491 Size: 16 32 154 32 367 32 858 Fragments arranged by size: 858 367 154 32 32 32 16 KasI G'GCGC_C Cuts at: 0 298 790 1213 1291 1491 Size: 298 492 423 78 200 Fragments arranged by size: 492 423 298 200 78 KpnI G_GTAC'C Cuts at: 0 937 1491 Size: 937 554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 997 1491 Size: 997 494 MscI TGG'CCA Cuts at: 0 415 1363 1400 1491 Size: 415 948 37 91 Fragments arranged by size: 948 415 91 37 MslI CAynn'nnrTG Cuts at: 0 82 409 1491 Size: 82 327 1082 Fragments arranged by size: 1082 327 82 MspA1I CmG'CkG Cuts at: 0 14 101 178 280 304 314 322 372 Size: 14 87 77 102 24 10 8 50 Cuts at: 372 473 742 901 927 1141 1333 1491 Size: 101 269 159 26 214 192 158 Fragments arranged by size: 269 214 192 159 158 102 101 87 77 50 26 24 14 10 8 NaeI GCC'GGC Cuts at: 0 851 1134 1296 1470 1491 Size: 851 283 162 174 21 Fragments arranged by size: 851 283 174 162 21 NarI GG'CG_CC Cuts at: 0 299 791 1214 1292 1491 Size: 299 492 423 78 199 Fragments arranged by size: 492 423 299 199 78 NcoI C'CATG_G Cuts at: 0 410 1491 Size: 410 1081 NgoMIV G'CCGG_C Cuts at: 0 849 1132 1294 1468 1491 Size: 849 283 162 174 23 Fragments arranged by size: 849 283 174 162 23 NotI GC'GGCC_GC Cuts at: 0 272 474 1491 Size: 272 202 1017 Fragments arranged by size: 1017 272 202 NspI r_CATG'y Cuts at: 0 630 1491 Size: 630 861 PasI CC'CwG_GG Cuts at: 0 6 1491 Size: 6 1485 PciI A'CATG_T Cuts at: 0 626 1491 Size: 626 865 PflMI CCAn_nnn'nTGG Cuts at: 0 1257 1359 1491 Size: 1257 102 132 Fragments arranged by size: 1257 132 102 PfoI T'CCnGG_A Cuts at: 0 1308 1491 Size: 1308 183 PpuMI rG'GwC_Cy Cuts at: 0 1336 1445 1491 Size: 1336 109 46 Fragments arranged by size: 1336 109 46 PshAI GACnn'nnGTC Cuts at: 0 342 1491 Size: 342 1149 PspOMI G'GGCC_C Cuts at: 0 130 325 1035 1041 1047 1491 Size: 130 195 710 6 6 444 Fragments arranged by size: 710 444 195 130 6 6 PstI C_TGCA'G Cuts at: 0 425 1491 Size: 425 1066 PvuII CAG'CTG Cuts at: 0 14 927 1491 Size: 14 913 564 Fragments arranged by size: 913 564 14 SacI G_AGCT'C Cuts at: 0 46 116 1491 Size: 46 70 1375 Fragments arranged by size: 1375 70 46 SacII CC_GC'GG Cuts at: 0 179 305 315 743 902 1142 1334 1491 Size: 179 126 10 428 159 240 192 157 Fragments arranged by size: 428 240 192 179 159 157 126 10 SanDI GG'GwC_CC Cuts at: 0 1445 1491 Size: 1445 46 SfcI C'TryA_G Cuts at: 0 421 1244 1491 Size: 421 823 247 Fragments arranged by size: 823 421 247 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1055 1491 Size: 1055 436 SfoI GGC'GCC Cuts at: 0 300 792 1215 1293 1491 Size: 300 492 423 78 198 Fragments arranged by size: 492 423 300 198 78 SgrAI Cr'CCGG_yG Cuts at: 0 1294 1491 Size: 1294 197 SmaI CCC'GGG Cuts at: 0 145 262 290 330 584 1040 1046 1289 Size: 145 117 28 40 254 456 6 243 Cuts at: 1289 1422 1491 Size: 133 69 Fragments arranged by size: 456 254 243 145 133 117 69 40 28 6 SmlI C'TyrA_G Cuts at: 0 1177 1491 Size: 1177 314 SrfI GCCC'GGGC Cuts at: 0 330 1040 1046 1491 Size: 330 710 6 445 Fragments arranged by size: 710 445 330 6 StuI AGG'CCT Cuts at: 0 1058 1233 1491 Size: 1058 175 258 Fragments arranged by size: 1058 258 175 StyI C'CwwG_G Cuts at: 0 410 1491 Size: 410 1081 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 710 1491 Size: 710 781 XmaI C'CCGG_G Cuts at: 0 143 260 288 328 582 1038 1044 1287 Size: 143 117 28 40 254 456 6 243 Cuts at: 1287 1420 1491 Size: 133 71 Fragments arranged by size: 456 254 243 143 133 117 71 40 28 6 Enzymes that do cut: Acc65I AfeI AflIII AhdI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BglI BglII Bme1580I BmgBI BpuEI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AflII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII EarI Eco57MI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI