################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRII EcoRV FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AfaI 360 GTAC GT^AC AlwNI 541 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 574 ATTAAT AT^TAAT AsnI 574 ATTAAT AT^TAAT Asp700I 916 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 637 GTGCTC G[AT]GC[AT]^C BalI 329 TGGCCA TGG^CCA BamHI 402 GGATCC G^GATCC BanII 460 GGGCTC G[GA]GC[CT]^C BbsI 703 GTCTTC GAAGAC(2/6) BbvCI 685 GCTGAGG CCTCAGC(-5/-2) BfaI 553 CTAG C^TAG BpuAI 703 GTCTTC GAAGAC(2/6) BseRI 121 CTCCTC GAGGAG(10/8) BsiHKAI 637 GTGCTC G[AT]GC[AT]^C BsmBI 154 CGTCTC CGTCTC(1/5) BsmFI 226 GTCCC GGGAC(10/14) BsrDI 237 CATTGC GCAATG(2/0) BstAPI 586 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 402 GGATCC [GA]^GATC[CT] DraI 180 TTTAAA TTT^AAA DraII 449 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 143 CCGTGG C^C[GA][CT]GG EaeI 329 TGGCCA [CT]^GGCC[GA] EcoO109I 449 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 568 GAATTC G^AATTC EcoT14I 698 CCAAGG C^C[AT][AT]GG HapII 693 CCGG C^CGG HincII 374 GTCAAC GT[CT]^[GA]AC HindII 374 GTCAAC GT[CT]^[GA]AC HindIII 58 AAGCTT A^AGCTT HpaII 693 CCGG C^CGG MaeI 553 CTAG C^TAG MaeII 604 ACGT A^CGT MflI 402 GGATCC [GA]^GATC[CT] MluNI 329 TGGCCA TGG^CCA MscI 329 TGGCCA TGG^CCA MspI 693 CCGG C^CGG PleI 509 GAGTC GAGTC(4/5) PshBI 574 ATTAAT AT^TAAT RsaI 360 GTAC GT^AC SapI 916 GAAGAGC GCTCTTC(1/4) StyI 698 CCAAGG C^C[AT][AT]GG TaqI 796 TCGA T^CGA TthHB8I 796 TCGA T^CGA XcmI 134 CCATCATTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 402 GGATCC [GA]^GATC[CT] XmnI 916 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 243 AAATTC [GA]^AATT[CT] 568 GAATTC AlwI 403 GATCC GGATC(4/5) 402 GGATC ApoI 243 AAATTC [GA]^AATT[CT] 568 GAATTC BmyI 460 GGGCTC G[AGT]GC[ACT]^C 637 GTGCTC BsaJI 143 CCGTGG C^C[ACGT][ACGT]GG 698 CCAAGG BsmAI 391 GAGAC GTCTC(1/5) 155 GTCTC BsmI 284 GCATTC GAATGC(1/-1) 595 GCATTC Bsp1286I 460 GGGCTC G[AGT]GC[ACT]^C 637 GTGCTC BspMI 98 ACCTGC ACCTGC(4/8) 712 ACCTGC DpnII 403 GATC ^GATC 627 GATC EcoT22I 74 ATGCAT ATGCA^T 466 ATGCAT MboI 403 GATC ^GATC 627 GATC MslI 138 CATTACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 339 CACAGTGATG MspA1I 683 CAGCTG C[AC]G^C[GT]G 752 CAGCTG MwoI 424 GCAGTGATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 586 GCACTGGCTGC NdeII 403 GATC ^GATC 627 GATC NlaIV 402 GGATCC GG[ACGT]^[ACGT]CC 449 GGGGCC NsiI 74 ATGCAT ATGCA^T 466 ATGCAT PshAI 148 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 696 GACCAAGGTC PstI 608 CTGCAG CTGCA^G 755 CTGCAG PvuII 683 CAGCTG CAG^CTG 752 CAGCTG Sau3AI 403 GATC ^GATC 627 GATC SphI 420 GCATGC GCATG^C 468 GCATGC Tsp45I 91 GTGAC ^GT[GC]AC 220 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI AvaII BbvI BsiYI BslI BsrI Cac8I Cfr13I DdeI EarI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MseI NlaIII NspI Sau96I SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI