(Circular) (Six-base) MAPSORT of: pFN21AE5080.seq Check: 8944 from: 1 to: 5668 pFN21AE5080 5668 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 5668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2279 3731 5115 5383 1632 Size: 419 228 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 419 268 228 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2551 3411 4983 2551 Size: 860 1572 3236 Fragments arranged by size: 3236 1572 860 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2248 2573 2977 3203 3355 5464 1493 Size: 755 325 404 226 152 2109 1697 Fragments arranged by size: 2109 1697 755 404 325 226 152 AseI AT'TA_AT Cuts at: 160 1032 2580 4222 160 Size: 872 1548 1642 1606 Fragments arranged by size: 1642 1606 1548 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2407 2998 3365 2407 Size: 591 367 4710 Fragments arranged by size: 4710 591 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 4444 618 Size: 325 217 627 1202 1455 1842 Fragments arranged by size: 1842 1455 1202 627 325 217 BanII G_rGCy'C Cuts at: 1391 2469 2987 1391 Size: 1078 518 4072 Fragments arranged by size: 4072 1078 518 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2701 928 Size: 971 802 3895 Fragments arranged by size: 3895 971 802 BbvCI CC'TCA_GC Cuts at: 2691 2691 Size: 5668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BfrBI ATG'CAT Cuts at: 2081 2473 2081 Size: 392 5276 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2230 3399 4357 Size: 416 53 83 399 24 813 1169 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1169 958 813 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4328 1239 Size: 3089 2579 Bpu10I CC'TnA_GC Cuts at: 2691 2691 Size: 5668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 4737 BseYI C'CCAG_C Cuts at: 2450 4871 5367 2450 Size: 2421 496 2751 Fragments arranged by size: 2751 2421 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 3729 3814 4885 2646 Size: 1083 85 1071 3429 Fragments arranged by size: 3429 1083 1071 85 BsmI GAATG_Cn' Cuts at: 2289 2600 3179 3272 2289 Size: 311 579 93 4685 Fragments arranged by size: 4685 579 311 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2165 2165 Size: 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2469 2646 2987 3729 3814 4885 1391 Size: 399 679 177 341 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 399 341 177 85 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2726 3023 844 Size: 1268 614 297 3489 Fragments arranged by size: 3489 1268 614 297 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2241 4163 4337 65 Size: 1183 993 1922 174 1396 Fragments arranged by size: 1922 1396 1183 993 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 2597 794 Size: 1803 3865 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2407 2998 3365 3750 3767 5219 5305 5317 Size: 435 591 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 591 435 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2148 513 Size: 552 939 144 4033 Fragments arranged by size: 4033 939 552 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2436 3184 3978 3998 2436 Size: 748 794 20 4106 Fragments arranged by size: 4106 794 748 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2187 2972 2187 Size: 785 4883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 3115 4004 Size: 54 1425 50 275 18 504 781 889 Cuts at: 4004 5633 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 781 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 2504 2916 3598 1353 Size: 852 299 412 682 3423 Fragments arranged by size: 3423 852 682 412 299 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2279 3731 4328 5115 5383 1239 Size: 393 419 228 1452 597 787 268 1524 Fragments arranged by size: 1524 1452 787 597 419 393 268 228 EcoO109I rG'GnC_Cy Cuts at: 2455 2455 Size: 5668 EcoRI G'AATT_C Cuts at: 2573 2977 3355 2573 Size: 404 378 4886 Fragments arranged by size: 4886 404 378 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 4815 1678 Size: 3137 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 4309 4341 4383 Size: 32 135 32 1211 32 1494 32 42 Cuts at: 4383 4415 1373 Size: 32 2626 Fragments arranged by size: 2626 1494 1211 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2381 3012 3258 669 Size: 988 281 443 631 246 3079 Fragments arranged by size: 3079 988 631 443 281 246 HindIII A'AGCT_T Cuts at: 748 2063 748 Size: 1315 4353 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2870 4782 4966 1305 Size: 1565 1912 184 2007 Fragments arranged by size: 2007 1912 1565 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 10 Size: 54 1425 343 504 3342 Fragments arranged by size: 3342 1425 504 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2348 3626 3985 4144 518 Size: 648 981 201 1278 359 159 2042 Fragments arranged by size: 2042 1278 981 648 359 201 159 MspA1I CmG'CkG Cuts at: 1606 2690 2759 3084 3761 4909 5154 1606 Size: 1084 69 325 677 1148 245 2120 Fragments arranged by size: 2120 1148 1084 677 325 245 69 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4177 Fragments arranged by size: 4177 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NsiI A_TGCA'T Cuts at: 2083 2475 2083 Size: 392 5276 NspI r_CATG'y Cuts at: 1132 1229 2081 2429 2477 3026 4571 1132 Size: 97 852 348 48 549 1545 2229 Fragments arranged by size: 2229 1545 852 549 348 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2157 2705 1613 Size: 544 548 4576 Fragments arranged by size: 4576 548 544 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 2617 2764 3020 830 Size: 1787 147 256 3478 Fragments arranged by size: 3478 1787 256 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2690 2759 2690 Size: 69 5599 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SapI GCTCTTCn'nnn_ Cuts at: 2916 2916 Size: 5668 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2419 2613 2667 2760 3016 4151 4832 Size: 219 1374 194 54 93 256 1135 681 Cuts at: 4832 5023 826 Size: 191 1471 Fragments arranged by size: 1471 1374 1135 681 256 219 194 191 93 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 2429 2477 3026 1132 Size: 1297 48 549 3774 Fragments arranged by size: 3774 1297 549 48 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2004 2703 3107 513 Size: 552 939 699 404 3074 Fragments arranged by size: 3074 939 699 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 2146 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2925 3797 2925 Size: 872 4796 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI