Restriction map for
pFN21AE5064
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AlwNI Aor51HI
ApaI AscI AseI AsnI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HpaI KasI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 18 GGTACC G^GTACC
AflIII 1430 ACGTGT A^C[GA][CT]GT
AhdI 612 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1075 CCTCAGG CC^T[ACGT]AGG
Asp718I 18 GGTACC G^GTACC
AspEI 612 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 18 GGTACC G^G[CT][GA]CC
BbrPI 1429 CACGTG CAC^GTG
BcgI 1005 CGAGTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 41 CCGGG CC^[GC]GG
BfaI 860 CTAG C^TAG
BpmI 1094 CTCCAG CTGGAG(16/14)
BsaAI 1429 CACGTG [CT]AC^GT[GA]
BsgI 1099 GTGCAG GTGCAG(16/14)
BsmFI 515 GTCCC GGGAC(10/14)
BstXI 518 CCATGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1075 CCTCAGG CC^T[ACGT]AGG
Cac8I 553 GCATGC GC[ACGT]^[ACGT]GC
CfoI 63 GCGC GCG^C
DrdI 229 GACATATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 612 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1075 CCTCAGG CC^T[ACGT]AGG
EcoT22I 551 ATGCAT ATGCA^T
HapII 41 CCGG C^CGG
HgaI 189 GCGTC GACGC(5/10)
HhaI 63 GCGC GCG^C
HinP1I 63 GCGC G^CGC
HincII 1018 GTCAAC GT[CT]^[GA]AC
HindII 1018 GTCAAC GT[CT]^[GA]AC
HindIII 448 AAGCTT A^AGCTT
HpaII 41 CCGG C^CGG
KpnI 18 GGTACC GGTAC^C
MaeI 860 CTAG C^TAG
MaeII 1430 ACGT A^CGT
MspI 41 CCGG C^CGG
NciI 41 CCGGG CC^[GC]GG
NsiI 551 ATGCAT ATGCA^T
NspI 553 GCATGC [GA]CATG^[CT]
PmaCI 1429 CACGTG CAC^GTG
PmlI 1429 CACGTG CAC^GTG
PvuII 1322 CAGCTG CAG^CTG
SmlI 821 CTCAAG C^T[CT][GA]AG
SphI 553 GCATGC GCATG^C
SspI 1033 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 19 GTAC GT^AC
473 GTAC
Alw44I 129 GTGCAC G^TGCAC
914 GTGCAC
ApaLI 129 GTGCAC G^TGCAC
914 GTGCAC
BbsI 1059 GAAGAC GAAGAC(2/6)
651 GTCTTC
BpuAI 1059 GAAGAC GAAGAC(2/6)
651 GTCTTC
BsmAI 492 GAGAC GTCTC(1/5)
421 GTCTC
BstYI 1411 GGATCT [GA]^GATC[CT]
1534 GGATCT
DraII 204 GGGTCCT [GA]G^G[ACGT]CC[CT]
618 AGGTCCT
DsaI 539 CCATGG C^C[GA][CT]GG
953 CCATGG
EcoO109I 204 GGGTCCT [GA]G^G[ACGT]CC[CT]
618 AGGTCCT
EcoT14I 539 CCATGG C^C[AT][AT]GG
953 CCATGG
HaeIII 334 GGCC GG^CC
996 GGCC
MflI 1411 GGATCT [GA]^GATC[CT]
1534 GGATCT
MspA1I 1088 CCGCTG C[AC]G^C[GT]G
1322 CAGCTG
NcoI 539 CCATGG C^CATGG
953 CCATGG
PflMI 953 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1525 CCACCTTCTGG
PleI 494 GACTC GAGTC(4/5)
649 GAGTC
PpuMI 204 GGGTCCT [GA]G^G[AT]CC[CT]
618 AGGTCCT
RsaI 19 GTAC GT^AC
473 GTAC
StyI 539 CCATGG C^C[AT][AT]GG
953 CCATGG
Van91I 953 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1525 CCACCTTCTGG
XcmI 518 CCATGAGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1031 CCAATATTCAAATGG
XhoII 1411 GGATCT [GA]^GATC[CT]
1534 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI