################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MaeII MaeIII MamI MfeI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 350 GGTACC G^GTACC AccI 1021 GTCTAC GT^[AC][GT]AC AccIII 459 TCCGGA T^CCGGA AfaI 351 GTAC GT^AC AlwI 291 GATCC GGATC(4/5) AlwNI 994 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 350 GGTACC G^GTACC BbvCI 142 CCTCAGC CCTCAGC(-5/-2) BglII 815 AGATCT A^GATCT BsaWI 459 TCCGGA [AT]^CCGG[AT] BseAI 459 TCCGGA T^CCGGA BsgI 790 CTGCAC GTGCAG(16/14) BsmBI 802 GAGACG CGTCTC(1/5) BspEI 459 TCCGGA T^CCGGA BsrBI 71 CCGCTC CCGCTC(-3/-3) BssSI 931 CACGAG CACGAG(-5/-1) BstF5I 921 CATCC GGATG(2/0) BstXI 340 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1033 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG FokI 921 CATCC GGATG(9/13) KpnI 350 GGTACC GGTAC^C MroI 459 TCCGGA T^CCGGA PaeR7I 724 CTCGAG C^TCGAG PshAI 804 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 962 CTGCAG CTGCA^G PvuII 853 CAGCTG CAG^CTG RsaI 351 GTAC GT^AC SapI 162 GCTCTTC GCTCTTC(1/4) SfaNI 905 GCATC GCATC(5/9) SfcI 962 CTGCAG C^T[GA][CT]AG SfiI 216 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 372 CGCCGGCG C[GA]^CCGG[CT]G SmaI 10 CCCGGG CCC^GGG StuI 410 AGGCCT AGG^CCT TspRI 467 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 218 CCAAGGGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 724 CTCGAG C^TCGAG XmaCI 10 CCCGGG C^CCGGG XmaI 10 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 235 GGCGCC G[GA]^CG[CT]C 573 GGCGCC Aor51HI 332 AGCGCT AGC^GCT 746 AGCGCT ApaI 224 GGGCCC GGGCC^C 974 GGGCCC AvaI 10 CCCGGG C^[CT]CG[GA]G 724 CTCGAG BbeI 235 GGCGCC GGCGC^C 573 GGCGCC BsaHI 235 GGCGCC G[GA]^CG[CT]C 573 GGCGCC BsaI 116 GAGACC GGTCTC(1/5) 1041 GGTCTC BseRI 426 CTCCTC GAGGAG(10/8) 532 GAGGAG BsmFI 84 GTCCC GGGAC(10/14) 786 GTCCC BsoBI 10 CCCGGG C^[CT]CG[GA]G 724 CTCGAG BsrI 632 ACTGG ACTGG(1/-1) 985 CCAGT BstYI 290 AGATCC [GA]^GATC[CT] 815 AGATCT EarI 163 CTCTTC CTCTTC(1/4) 1044 CTCTTC Eco47III 332 AGCGCT AGC^GCT 746 AGCGCT EcoT14I 77 CCAAGG C^C[AT][AT]GG 218 CCAAGG Hin1I 235 GGCGCC G[GA]^CG[CT]C 573 GGCGCC HphI 797 TCACC GGTGA(8/7) 1004 TCACC KasI 235 GGCGCC G^GCGCC 573 GGCGCC Ksp632I 163 CTCTTC CTCTTC(1/4) 1044 CTCTTC MflI 290 AGATCC [GA]^GATC[CT] 815 AGATCT NarI 235 GGCGCC GG^CGCC 573 GGCGCC NlaIII 741 CATG CATG^ 889 CATG SfoI 235 GGCGCC GGC^GCC 573 GGCGCC SmlI 472 CTCAAG C^T[CT][GA]AG 724 CTCGAG StyI 77 CCAAGG C^C[AT][AT]GG 218 CCAAGG TaqI 725 TCGA T^CGA 737 TCGA TthHB8I 725 TCGA T^CGA 737 TCGA XhoII 290 AGATCC [GA]^GATC[CT] 815 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AluI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BsiEI BsiYI BslI BsmAI Bsp1286I BspMI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI KspI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PleI SacII Sau3AI Sau96I ScrFI TseI