(Circular) (Six-base) MAPSORT of: pFN21AB8968.seq Check: 943 from: 1 to: 5776 pFN21AB8968 5776 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2254 2254 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3027 3119 1937 Size: 1090 92 4594 Fragments arranged by size: 4594 1090 92 Acc65I G'GTAC_C Cuts at: 2355 3097 2355 Size: 742 5034 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 3839 5223 5491 1632 Size: 789 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 789 268 AfeI AGC'GCT Cuts at: 2339 2753 2339 Size: 414 5362 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2328 1184 Size: 1144 4632 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2078 Size: 32 5744 AlwNI CAG_nnn'CTG Cuts at: 3004 3519 5091 3004 Size: 515 1572 3689 Fragments arranged by size: 3689 1572 515 ApaI G_GGCC'C Cuts at: 2233 2983 2233 Size: 750 5026 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2729 3101 1870 Size: 73 72 714 372 4545 Fragments arranged by size: 4545 714 372 73 72 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2240 2355 2578 2920 Size: 325 217 627 246 207 115 223 342 Cuts at: 2920 2986 3097 4552 618 Size: 66 111 1455 1842 Fragments arranged by size: 1842 1455 627 342 325 246 223 217 207 115 111 66 BanII G_rGCy'C Cuts at: 1391 2233 2519 2983 3095 1391 Size: 842 286 464 112 4072 Fragments arranged by size: 4072 842 464 286 112 BbeI G_GCGC'C Cuts at: 2244 2582 2244 Size: 338 5438 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 2695 2696 2713 928 Size: 971 741 55 1 17 3991 Fragments arranged by size: 3991 971 741 55 17 1 BbvCI CC'TCA_GC Cuts at: 2148 2148 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3967 4001 1903 Size: 34 50 34 1946 34 3678 Fragments arranged by size: 3678 1946 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 2228 2783 Size: 107 122 71 631 605 547 9 555 Cuts at: 2783 2978 4387 136 Size: 195 1409 1525 Fragments arranged by size: 1525 1409 631 605 555 547 195 122 107 71 9 BglII A'GATC_T Cuts at: 2820 5771 2820 Size: 2951 2825 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2233 2925 2983 3837 4993 1790 Size: 443 692 58 854 1156 2573 Fragments arranged by size: 2573 1156 854 692 443 58 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 2563 2524 Size: 39 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2354 2555 2894 4436 1239 Size: 1016 99 201 339 1542 2579 Fragments arranged by size: 2579 1542 1016 339 201 99 Bpu10I CC'TnA_GC Cuts at: 2142 2148 2888 2142 Size: 6 740 5030 Fragments arranged by size: 5030 740 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 3901 4766 5064 2462 Size: 1439 865 298 3174 Fragments arranged by size: 3174 1439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2115 3052 4439 882 Size: 617 616 937 1387 2219 Fragments arranged by size: 2219 1387 937 617 616 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 2579 3583 3965 Size: 53 83 186 936 708 338 1004 382 Cuts at: 3965 4600 275 Size: 635 1451 Fragments arranged by size: 1451 1004 936 708 635 382 338 186 83 53 BsaWI w'CCGG_w Cuts at: 2464 4209 4607 4881 5028 5439 2464 Size: 1745 398 274 147 411 2801 Fragments arranged by size: 2801 1745 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 1178 Size: 30 165 30 675 30 4846 Fragments arranged by size: 4846 675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2422 2552 1186 Size: 1236 130 4410 Fragments arranged by size: 4410 1236 130 BseYI C'CCAG_C Cuts at: 2344 4979 5475 2344 Size: 2635 496 2645 Fragments arranged by size: 2645 2635 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 1163 Size: 573 1044 4159 Fragments arranged by size: 4159 1044 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2592 2952 3071 3075 3226 Size: 275 186 134 457 360 119 4 151 Cuts at: 3226 3987 5015 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 457 360 275 186 151 134 119 4 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2801 2801 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2519 2925 2983 3095 3837 3922 Size: 399 443 286 406 58 112 742 85 Cuts at: 3922 4993 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 443 406 399 286 112 85 58 BspEI T'CCGG_A Cuts at: 2464 5439 2464 Size: 2975 2801 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 2254 2803 3131 844 Size: 1274 136 549 328 3489 Fragments arranged by size: 3489 1274 549 328 136 BsrBI CCG'CTC Cuts at: 1394 1823 2078 3663 1394 Size: 429 255 1585 3507 Fragments arranged by size: 3507 1585 429 255 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2130 2378 2671 2777 3070 4420 Size: 110 220 100 248 293 106 293 1350 Cuts at: 4420 4607 1700 Size: 187 2869 Fragments arranged by size: 2869 1350 293 293 248 220 187 110 106 100 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2936 3836 4848 2936 Size: 900 1012 3864 Fragments arranged by size: 3864 1012 900 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2352 2352 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2295 2820 3106 3473 3858 3875 5327 5413 Size: 323 525 286 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 525 385 367 346 323 286 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2159 2214 2244 2898 2973 513 Size: 552 1094 55 30 654 75 3316 Fragments arranged by size: 3316 1094 654 552 75 55 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2471 3292 4086 4106 2471 Size: 821 794 20 4141 Fragments arranged by size: 4141 821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 3043 3043 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 2220 2383 Size: 54 1425 50 275 18 302 88 163 Cuts at: 2383 2589 2616 2784 2880 2949 3068 3223 4112 Size: 206 27 168 96 69 119 155 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 302 275 206 168 163 155 119 96 88 69 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2132 2589 2949 3068 3223 1537 Size: 275 320 457 360 119 155 4090 Fragments arranged by size: 4090 457 360 320 275 155 119 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 3055 3706 1353 Size: 821 881 651 3423 Fragments arranged by size: 3423 881 821 651 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2882 4363 4737 4883 387 Size: 1064 138 1293 1481 374 146 1280 Fragments arranged by size: 1481 1293 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2354 2421 2555 2894 3839 4436 Size: 393 623 99 67 134 339 945 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 945 787 623 597 393 339 268 134 99 67 EcoO109I rG'GnC_Cy Cuts at: 2229 2230 2979 2980 2229 Size: 1 749 1 5025 Fragments arranged by size: 5025 749 1 1 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2244 2341 2582 2652 2755 4923 1678 Size: 566 97 241 70 103 2168 2531 Fragments arranged by size: 2531 2168 566 241 103 97 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 4417 4449 4491 Size: 32 135 32 872 32 1941 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1941 872 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2240 2578 2240 Size: 338 5438 KpnI G_GTAC'C Cuts at: 2359 3101 2359 Size: 742 5034 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2210 2216 2246 2860 2900 2975 3004 Size: 598 6 6 30 614 40 75 29 Cuts at: 3004 3061 3192 3869 5017 5262 1606 Size: 57 131 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 614 598 245 131 75 57 40 30 29 6 6 NaeI GCC'GGC Cuts at: 2032 2380 2673 2779 2032 Size: 348 293 106 5029 Fragments arranged by size: 5029 348 293 106 NarI GG'CG_CC Cuts at: 2241 2579 2241 Size: 338 5438 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NgoMIV G'CCGG_C Cuts at: 2030 2378 2671 2777 2030 Size: 348 293 106 5029 Fragments arranged by size: 5029 348 293 106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2078 2110 3845 3877 5358 5390 2078 Size: 32 1735 32 1481 32 2464 Fragments arranged by size: 2464 1735 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2813 1613 Size: 1200 4576 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2229 2979 2229 Size: 750 5026 PspXI vC'TCGA_Gb Cuts at: 2729 2729 Size: 5776 PstI C_TGCA'G Cuts at: 830 2971 3128 830 Size: 2141 157 3478 Fragments arranged by size: 3478 2141 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2860 2860 Size: 5776 SacII CC_GC'GG Cuts at: 2217 2247 2901 2976 2217 Size: 30 654 75 5017 Fragments arranged by size: 5017 654 75 30 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2174 2174 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2967 3124 4259 4940 5131 826 Size: 219 1922 157 1135 681 191 1471 Fragments arranged by size: 1922 1471 1135 681 219 191 157 SfiI GGCCn_nnn'nGGCC Cuts at: 2228 2228 Size: 5776 SfoI GGC'GCC Cuts at: 2242 2580 2242 Size: 338 5438 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 2378 1700 Size: 678 5098 SmaI CCC'GGG Cuts at: 1872 2017 3103 1872 Size: 145 1086 4545 Fragments arranged by size: 4545 1086 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 2729 3880 4781 5043 820 Size: 197 926 534 252 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 534 262 252 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2417 1517 Size: 900 4876 StyI C'CwwG_G Cuts at: 513 1065 2082 2223 3215 513 Size: 552 1017 141 992 3074 Fragments arranged by size: 3074 1017 992 552 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2230 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 2729 1943 Size: 786 4990 XmaI C'CCGG_G Cuts at: 1870 2015 3101 1870 Size: 145 1086 4545 Fragments arranged by size: 4545 1086 145 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SexAI SrfI SwaI