(Linear) (Six-base) MAPSORT of: KIBB8968.seq Check: 571 from: 1 to: 1071 KIBB8968.orf With 182 enzymes: SgfI * December 5, 2014 12:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 249 1071 Size: 249 822 AccI GT'mk_AC Cuts at: 0 1022 1071 Size: 1022 49 Acc65I G'GTAC_C Cuts at: 0 350 1071 Size: 350 721 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1071 Size: 416 655 AfeI AGC'GCT Cuts at: 0 334 748 1071 Size: 334 414 323 Fragments arranged by size: 414 334 323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 323 1071 Size: 323 748 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 73 105 1071 Size: 73 32 966 Fragments arranged by size: 966 73 32 AlwNI CAG_nnn'CTG Cuts at: 0 999 1071 Size: 999 72 ApaI G_GGCC'C Cuts at: 0 228 978 1071 Size: 228 750 93 Fragments arranged by size: 750 228 93 AvaI C'yCGr_G Cuts at: 0 10 724 1071 Size: 10 714 347 Fragments arranged by size: 714 347 10 BanI G'GyrC_C Cuts at: 0 28 235 350 573 915 981 1071 Size: 28 207 115 223 342 66 90 Fragments arranged by size: 342 223 207 115 90 66 28 BanII G_rGCy'C Cuts at: 0 228 514 978 1071 Size: 228 286 464 93 Fragments arranged by size: 464 286 228 93 BbeI G_GCGC'C Cuts at: 0 239 577 1071 Size: 239 338 494 Fragments arranged by size: 494 338 239 BbsI GAAGACnn'nnnn_ Cuts at: 0 635 690 691 708 1071 Size: 635 55 1 17 363 Fragments arranged by size: 635 363 55 17 1 BbvCI CC'TCA_GC Cuts at: 0 143 1071 Size: 143 928 BglI GCCn_nnn'nGGC Cuts at: 0 214 223 778 973 1071 Size: 214 9 555 195 98 Fragments arranged by size: 555 214 195 98 9 BglII A'GATC_T Cuts at: 0 815 1071 Size: 815 256 Bme1580I G_kGCm'C Cuts at: 0 228 920 978 1071 Size: 228 692 58 93 Fragments arranged by size: 692 228 93 58 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 519 558 1071 Size: 519 39 513 Fragments arranged by size: 519 513 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 349 550 889 1071 Size: 250 99 201 339 182 Fragments arranged by size: 339 250 201 182 99 Bpu10I CC'TnA_GC Cuts at: 0 137 143 883 1071 Size: 137 6 740 188 Fragments arranged by size: 740 188 137 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 457 1071 Size: 457 614 BsaI GGTCTCn'nnnn_ Cuts at: 0 110 1047 1071 Size: 110 937 24 Fragments arranged by size: 937 110 24 BsaHI Gr'CG_yC Cuts at: 0 236 574 1071 Size: 236 338 497 Fragments arranged by size: 497 338 236 BsaWI w'CCGG_w Cuts at: 0 459 1071 Size: 459 612 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 73 103 1071 Size: 73 30 968 Fragments arranged by size: 968 73 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 417 547 1071 Size: 417 130 524 Fragments arranged by size: 524 417 130 BseYI C'CCAG_C Cuts at: 0 339 1071 Size: 339 732 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 775 1071 Size: 775 296 BsiEI CG_ry'CG Cuts at: 0 130 587 947 1066 1071 Size: 130 457 360 119 5 Fragments arranged by size: 457 360 130 119 5 BsmBI CGTCTCn'nnnn_ Cuts at: 0 796 1071 Size: 796 275 Bsp1286I G_dGCh'C Cuts at: 0 228 514 920 978 1071 Size: 228 286 406 58 93 Fragments arranged by size: 406 286 228 93 58 BspEI T'CCGG_A Cuts at: 0 459 1071 Size: 459 612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 113 249 798 1071 Size: 113 136 549 273 Fragments arranged by size: 549 273 136 113 BsrBI CCG'CTC Cuts at: 0 73 1071 Size: 73 998 BsrFI r'CCGG_y Cuts at: 0 25 125 373 666 772 1065 1071 Size: 25 100 248 293 106 293 6 Fragments arranged by size: 293 293 248 106 100 25 6 BssSI C'ACGA_G Cuts at: 0 931 1071 Size: 931 140 BstXI CCAn_nnnn'nTGG Cuts at: 0 347 1071 Size: 347 724 BstYI r'GATC_y Cuts at: 0 290 815 1071 Size: 290 525 256 Fragments arranged by size: 525 290 256 BtgI C'CryG_G Cuts at: 0 154 209 239 893 968 1071 Size: 154 55 30 654 75 103 Fragments arranged by size: 654 154 103 75 55 30 BtsI GCAGTG_nn' Cuts at: 0 466 1071 Size: 466 605 DraIII CAC_nnn'GTG Cuts at: 0 1038 1071 Size: 1038 33 EaeI y'GGCC_r Cuts at: 0 127 215 378 584 611 779 875 944 Size: 127 88 163 206 27 168 96 69 Cuts at: 944 1063 1071 Size: 119 8 Fragments arranged by size: 206 168 163 127 119 96 88 69 27 8 EagI C'GGCC_G Cuts at: 0 127 584 944 1063 1071 Size: 127 457 360 119 8 Fragments arranged by size: 457 360 127 119 8 EarI CTCTTCn'nnn_ Cuts at: 0 169 1050 1071 Size: 169 881 21 Fragments arranged by size: 881 169 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 877 1071 Size: 877 194 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 349 416 550 889 1071 Size: 250 99 67 134 339 182 Fragments arranged by size: 339 250 182 134 99 67 EcoO109I rG'GnC_Cy Cuts at: 0 224 225 974 975 1071 Size: 224 1 749 1 96 Fragments arranged by size: 749 224 96 1 1 HaeII r_GCGC'y Cuts at: 0 239 336 577 647 750 1071 Size: 239 97 241 70 103 321 Fragments arranged by size: 321 241 239 103 97 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 439 471 1071 Size: 439 32 600 Fragments arranged by size: 600 439 32 KasI G'GCGC_C Cuts at: 0 235 573 1071 Size: 235 338 498 Fragments arranged by size: 498 338 235 KpnI G_GTAC'C Cuts at: 0 354 1071 Size: 354 717 MspA1I CmG'CkG Cuts at: 0 199 205 211 241 855 895 970 999 Size: 199 6 6 30 614 40 75 29 Cuts at: 999 1056 1071 Size: 57 15 Fragments arranged by size: 614 199 75 57 40 30 29 15 6 6 NaeI GCC'GGC Cuts at: 0 27 375 668 774 1071 Size: 27 348 293 106 297 Fragments arranged by size: 348 297 293 106 27 NarI GG'CG_CC Cuts at: 0 236 574 1071 Size: 236 338 497 Fragments arranged by size: 497 338 236 NgoMIV G'CCGG_C Cuts at: 0 25 373 666 772 1071 Size: 25 348 293 106 299 Fragments arranged by size: 348 299 293 106 25 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 73 105 1071 Size: 73 32 966 Fragments arranged by size: 966 73 32 PshAI GACnn'nnGTC Cuts at: 0 808 1071 Size: 808 263 PspOMI G'GGCC_C Cuts at: 0 224 974 1071 Size: 224 750 97 Fragments arranged by size: 750 224 97 PspXI vC'TCGA_Gb Cuts at: 0 724 1071 Size: 724 347 PstI C_TGCA'G Cuts at: 0 966 1071 Size: 966 105 PvuII CAG'CTG Cuts at: 0 855 1071 Size: 855 216 SacII CC_GC'GG Cuts at: 0 212 242 896 971 1071 Size: 212 30 654 75 100 Fragments arranged by size: 654 212 100 75 30 SapI GCTCTTCn'nnn_ Cuts at: 0 169 1071 Size: 169 902 SfcI C'TryA_G Cuts at: 0 962 1071 Size: 962 109 SfiI GGCCn_nnn'nGGCC Cuts at: 0 223 1071 Size: 223 848 SfoI GGC'GCC Cuts at: 0 237 575 1071 Size: 237 338 496 Fragments arranged by size: 496 338 237 SgrAI Cr'CCGG_yG Cuts at: 0 373 1071 Size: 373 698 SmaI CCC'GGG Cuts at: 0 12 1071 Size: 12 1059 SmlI C'TyrA_G Cuts at: 0 472 724 1071 Size: 472 252 347 Fragments arranged by size: 472 347 252 StuI AGG'CCT Cuts at: 0 412 1071 Size: 412 659 StyI C'CwwG_G Cuts at: 0 77 218 1071 Size: 77 141 853 Fragments arranged by size: 853 141 77 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 225 1071 Size: 225 846 XhoI C'TCGA_G Cuts at: 0 724 1071 Size: 724 347 XmaI C'CCGG_G Cuts at: 0 10 1071 Size: 10 1061 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AlfI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MspA1I NaeI NarI NgoMIV PpiI PshAI PspOMI PspXI PstI PvuII SacII SapI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI XcmI XhoI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BsaAI BsaBI BsiHKAI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PsiI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XmnI ZraI