################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MflI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 247 CGCG CG^CG AccIII 6 TCCGGA T^CCGGA AcyI 882 GACGCC G[GA]^CG[CT]C AlwI 164 GGATC GGATC(4/5) ApaI 122 GGGCCC GGGCC^C BanI 729 GGTGCC G^G[CT][GA]CC BbsI 754 GTCTTC GAAGAC(2/6) BbvCI 565 GCTGAGG CCTCAGC(-5/-2) BclI 416 TGATCA T^GATCA BfaI 562 CTAG C^TAG BglI 298 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 264 CTCCAG CTGGAG(16/14) BpuAI 754 GTCTTC GAAGAC(2/6) BsaBI 744 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 882 GACGCC G[GA]^CG[CT]C BsaWI 6 TCCGGA [AT]^CCGG[AT] BseAI 6 TCCGGA T^CCGGA BsiEI 798 CGACCG CG[GA][CT]^CG BspEI 6 TCCGGA T^CCGGA BspHI 413 TCATGA T^CATGA BspMI 424 ACCTGC ACCTGC(4/8) BsrBI 774 CCGCTC CCGCTC(-3/-3) BsrDI 307 GCAATG GCAATG(2/0) BstAPI 204 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 247 CGCG CG^CG DdeI 566 CTGAG C^T[ACGT]AG DsaI 299 CCATGG C^C[GA][CT]GG EaeI 168 CGGCCA [CT]^GGCC[GA] EarI 556 CTCTTC CTCTTC(1/4) EcoNI 561 CCTAGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1 ATGCAT ATGCA^T FbaI 416 TGATCA T^GATCA HaeII 216 GGCGCT [GA]GCGC^[CT] Hin1I 882 GACGCC G[GA]^CG[CT]C HindIII 253 AAGCTT A^AGCTT HinfI 484 GAGTC G^A[ACGT]TC Ksp632I 556 CTCTTC CTCTTC(1/4) MaeI 562 CTAG C^TAG MamI 744 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 6 TCCGGA T^CCGGA MseI 257 TTAA T^TAA MvnI 247 CGCG CG^CG NcoI 299 CCATGG C^CATGG NsiI 1 ATGCAT ATGCA^T NspI 500 GCATGC [GA]CATG^[CT] PleI 484 GAGTC GAGTC(4/5) RcaI 413 TCATGA T^CATGA SacI 814 GAGCTC GAGCT^C SapI 555 GCTCTTC GCTCTTC(1/4) SphI 500 GCATGC GCATG^C Tru9I 257 TTAA T^TAA Tsp45I 148 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 397 GTGCAC G^TGCAC 460 GTGCAC ApaLI 397 GTGCAC G^TGCAC 460 GTGCAC BsgI 375 CTGCAC GTGCAG(16/14) 319 GTGCAG BstF5I 4 CATCC GGATG(2/0) 429 CATCC BstXI 380 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 719 CCACCATCATGG DraII 121 GGGGCCC [GA]G^G[ACGT]CC[CT] 122 GGGCCCC EcoO109I 121 GGGGCCC [GA]G^G[ACGT]CC[CT] 122 GGGCCCC EcoT14I 299 CCATGG C^C[AT][AT]GG 625 CCAAGG FokI 4 CATCC GGATG(9/13) 429 CATCC HgaI 328 GACGC GACGC(5/10) 882 GACGC MaeII 458 ACGT A^CGT 668 ACGT MaeIII 148 GTCAC ^GT[ACGT]AC 176 GTTAC MboII 557 TCTTC GAAGA(8/7) 755 TCTTC SmaI 117 CCCGGG CCC^GGG 690 CCCGGG SmlI 240 CTTGAG C^T[CT][GA]AG 604 CTCAAG StyI 299 CCATGG C^C[AT][AT]GG 625 CCAAGG XcmI 299 CCATGGTGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 380 CCACAGTGCTGGTGG XmaCI 117 CCCGGG C^CCGGG 690 CCCGGG XmaI 117 CCCGGG C^CCGGG 690 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I