(Circular) (Six-base) MAPSORT of: pFN21AE5023.seq Check: 3372 from: 1 to: 6106 pFN21AE5023 6106 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3145 3145 Size: 6106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4169 5553 5821 1632 Size: 2537 1384 268 1917 Fragments arranged by size: 2537 1917 1384 268 AfeI AGC'GCT Cuts at: 2357 2357 Size: 6106 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 2796 5005 5982 1203 Size: 1593 2209 977 1327 Fragments arranged by size: 2209 1593 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 2579 1166 Size: 1413 4693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 3849 5421 3849 Size: 1572 4534 ApaI G_GGCC'C Cuts at: 2294 3347 2294 Size: 1053 5053 ApaLI G'TGCA_C Cuts at: 3026 4163 5319 3026 Size: 1137 1156 3813 Fragments arranged by size: 3813 1156 1137 ApoI r'AATT_y Cuts at: 1493 3415 3641 3793 5902 1493 Size: 1922 226 152 2109 1697 Fragments arranged by size: 2109 1922 1697 226 152 AscI GG'CGCG_CC Cuts at: 2345 2912 2345 Size: 567 5539 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2463 2521 2827 3149 3281 3431 Size: 73 83 437 58 306 322 132 150 Cuts at: 3431 1870 Size: 4545 Fragments arranged by size: 4545 437 322 306 150 132 83 73 58 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2490 3427 4882 618 Size: 325 217 627 703 937 1455 1842 Fragments arranged by size: 1842 1455 937 703 627 325 217 BanII G_rGCy'C Cuts at: 1391 2294 2444 3252 3347 3425 1391 Size: 903 150 808 95 78 4072 Fragments arranged by size: 4072 903 808 150 95 78 BbeI G_GCGC'C Cuts at: 2494 2494 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BbvCI CC'TCA_GC Cuts at: 3179 3179 Size: 6106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2524 2558 2644 2678 4297 4331 1903 Size: 34 587 34 86 34 1619 34 3678 Fragments arranged by size: 3678 1619 587 86 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 2252 2576 Size: 107 122 71 631 605 439 141 324 Cuts at: 2576 4717 136 Size: 2141 1525 Fragments arranged by size: 2141 1525 631 605 439 324 141 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3167 3523 3167 Size: 356 5750 Bme1580I G_kGCm'C Cuts at: 1790 2294 3030 3078 3347 4167 5323 1790 Size: 504 736 48 269 820 1156 2573 Fragments arranged by size: 2573 1156 820 736 504 269 48 BmgBI CAC'GTC Cuts at: 1318 2062 2197 2503 1318 Size: 744 135 306 4921 Fragments arranged by size: 4921 744 306 135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 3837 4795 Size: 416 53 83 399 24 921 1499 958 Cuts at: 4795 442 Size: 1753 Fragments arranged by size: 1753 1499 958 921 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3352 3352 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2372 2690 2867 3052 4766 1239 Size: 917 216 318 177 185 1714 2579 Fragments arranged by size: 2579 1714 917 318 216 185 177 Bpu10I CC'TnA_GC Cuts at: 2594 3179 2594 Size: 585 5521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3236 4231 5096 5394 3236 Size: 995 865 298 3948 Fragments arranged by size: 3948 995 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4769 882 Size: 617 1167 2103 2219 Fragments arranged by size: 2219 2103 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 2797 2899 3897 493 Size: 711 1593 102 998 2702 Fragments arranged by size: 2702 1593 998 711 102 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2491 3913 4295 4930 Size: 53 83 186 936 958 1422 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1451 1422 958 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2663 2693 1178 Size: 30 165 30 1260 30 4591 Fragments arranged by size: 4591 1260 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2405 2636 2639 1186 Size: 1216 3 231 3 4653 Fragments arranged by size: 4653 1216 231 3 3 BseYI C'CCAG_C Cuts at: 2809 2923 2938 3046 5309 5805 2809 Size: 114 15 108 2263 496 3110 Fragments arranged by size: 3110 2263 496 114 108 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 2400 2485 2580 3556 4317 Size: 275 186 223 176 85 95 976 761 Cuts at: 4317 5345 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 223 186 176 95 85 BsiHKAI G_wGCw'C Cuts at: 2600 2642 2895 3030 3252 4167 4252 5323 2600 Size: 42 253 135 222 915 85 1071 3383 Fragments arranged by size: 3383 1071 915 253 222 135 85 42 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 2444 2600 2642 2895 3030 3078 Size: 399 504 150 156 42 253 135 48 Cuts at: 3078 3252 3347 3425 4167 4252 5323 1391 Size: 174 95 78 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 253 174 156 150 135 95 85 78 48 42 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 3145 3461 844 Size: 1334 967 316 3489 Fragments arranged by size: 3489 1334 967 316 BsrBI CCG'CTC Cuts at: 1394 1823 2629 3993 1394 Size: 429 806 1364 3507 Fragments arranged by size: 3507 1364 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2484 3286 4750 4937 1700 Size: 110 674 802 1464 187 2869 Fragments arranged by size: 2869 1464 802 674 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 2129 2146 2263 2298 2345 2447 2449 2749 Size: 204 17 117 35 47 102 2 300 Cuts at: 2749 2751 2851 2912 2961 2963 3051 3053 3268 Size: 2 100 61 49 2 88 2 215 Cuts at: 3268 1925 Size: 4763 Fragments arranged by size: 4763 300 215 204 117 102 100 88 61 49 47 35 17 2 2 2 2 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 2232 794 Size: 1438 4668 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 3208 1184 Size: 2024 4082 BstXI CCAn_nnnn'nTGG Cuts at: 2845 2845 Size: 6106 BstYI r'GATC_y Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101 Size: 1464 367 385 17 1452 86 12 346 Cuts at: 6101 1972 Size: 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2155 2580 3003 3114 3375 Size: 552 939 8 143 425 423 111 261 Cuts at: 3375 513 Size: 3244 Fragments arranged by size: 3244 939 552 425 423 261 143 111 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2131 487 Size: 683 56 905 4462 Fragments arranged by size: 4462 905 683 56 BtsI GCAGTG_nn' Cuts at: 3622 4416 4436 3622 Size: 794 20 5292 Fragments arranged by size: 5292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2397 2482 Size: 54 1425 50 275 18 328 239 85 Cuts at: 2482 2568 2577 3000 3033 3273 3315 3363 3553 Size: 86 9 423 33 240 42 48 190 Cuts at: 3553 4442 6071 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 423 328 275 240 239 190 86 85 54 50 48 43 42 33 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2397 2482 2577 3553 1537 Size: 275 585 85 95 976 4090 Fragments arranged by size: 4090 976 585 275 95 85 EarI CTCTTCn'nnn_ Cuts at: 1353 2546 3017 4036 1353 Size: 1193 471 1019 3423 Fragments arranged by size: 3423 1193 1019 471 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3250 3250 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2372 2690 2867 3052 4169 4766 Size: 393 524 216 318 177 185 1117 597 Cuts at: 4766 5553 5821 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1117 787 597 524 393 318 268 216 185 177 EcoNI CCTnn'n_nnAGG Cuts at: 3296 3296 Size: 6106 EcoO109I rG'GnC_Cy Cuts at: 2046 2290 2459 3343 3344 2046 Size: 244 169 884 1 4808 Fragments arranged by size: 4808 884 244 169 1 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 6106 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2143 2258 2359 2494 2783 3011 3167 5253 Size: 465 115 101 135 289 228 156 2086 Cuts at: 5253 1678 Size: 2531 Fragments arranged by size: 2531 2086 465 289 228 156 135 115 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 4747 4779 4821 Size: 32 135 32 537 32 2606 32 42 Cuts at: 4821 4853 1373 Size: 32 2626 Fragments arranged by size: 2626 2606 537 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2490 2490 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3002 10 Size: 54 1425 343 1170 3114 Fragments arranged by size: 3114 1425 1170 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2579 3104 4064 4423 4582 518 Size: 648 906 507 525 960 359 159 2042 Fragments arranged by size: 2042 960 906 648 525 507 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2958 3183 3377 3522 4199 5347 5592 Size: 551 801 225 194 145 677 1148 245 Cuts at: 5592 1606 Size: 2120 Fragments arranged by size: 2120 1148 801 677 551 245 225 194 145 NaeI GCC'GGC Cuts at: 2486 3288 2486 Size: 802 5304 NarI GG'CG_CC Cuts at: 2491 2491 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 2012 3003 513 Size: 552 939 8 991 3616 Fragments arranged by size: 3616 991 939 552 8 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NgoMIV G'CCGG_C Cuts at: 2484 3286 2484 Size: 802 5304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 3464 5009 1132 Size: 97 2235 1545 2229 Fragments arranged by size: 2235 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2455 1348 Size: 1107 4999 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 3087 1108 Size: 1815 164 4127 Fragments arranged by size: 4127 1815 164 PfoI T'CCnGG_A Cuts at: 2844 2844 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2459 2046 Size: 413 5693 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 2290 3343 2290 Size: 1053 5053 PstI C_TGCA'G Cuts at: 830 2478 2724 3297 3458 830 Size: 1648 246 573 161 3478 Fragments arranged by size: 3478 1648 573 246 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SacI G_AGCT'C Cuts at: 3252 3252 Size: 6106 SacII CC_GC'GG Cuts at: 2158 3378 2158 Size: 1220 4886 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SanDI GG'GwC_CC Cuts at: 2046 2046 Size: 6106 SapI GCTCTTCn'nnn_ Cuts at: 2546 3017 2546 Size: 471 5635 SbfI CC_TGCA'GG Cuts at: 2724 3297 3458 2724 Size: 573 161 5372 Fragments arranged by size: 5372 573 161 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2474 2720 3293 3454 4589 5270 5461 Size: 219 1429 246 573 161 1135 681 191 Cuts at: 5461 826 Size: 1471 Fragments arranged by size: 1471 1429 1135 681 573 246 219 191 161 SfiI GGCCn_nnn'nGGCC Cuts at: 2576 2576 Size: 6106 SfoI GGC'GCC Cuts at: 2492 2492 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 2028 2829 3151 3433 1872 Size: 156 801 322 282 4545 Fragments arranged by size: 4545 801 322 282 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 3251 4210 5111 5373 820 Size: 197 926 1308 959 901 262 1553 Fragments arranged by size: 1553 1308 959 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SrfI GCCC'GGGC Cuts at: 2829 3151 2829 Size: 322 5784 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2004 2012 3003 3545 513 Size: 552 939 8 991 542 3074 Fragments arranged by size: 3074 991 939 552 542 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3379 4133 4150 4303 4488 4933 325 Size: 1255 1799 754 17 153 185 445 1498 Fragments arranged by size: 1799 1498 1255 754 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4352 6064 96 Size: 274 80 33 51 1443 2375 1712 138 Fragments arranged by size: 2375 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2663 2695 3371 3403 6066 6098 2663 Size: 32 676 32 2663 32 2671 Fragments arranged by size: 2671 2663 676 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3131 1316 Size: 1815 4291 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3112 3112 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 2026 2827 3149 3431 1870 Size: 156 801 322 282 4545 Fragments arranged by size: 4545 801 322 282 156 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SexAI SwaI