################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KspI MaeII MamI MflI MluI MluNI MroI MscI MslI MspI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 231 GGTACC G^GTACC AciI 545 CCGC CCGC(-3/-1) AlwNI 1091 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 694 CCTCAGG CC^T[ACGT]AGG Asp718I 231 GGTACC G^GTACC AvaI 557 CTCGAG C^[CT]CG[GA]G AvaII 899 GGTCC G^G[AT]CC BanI 231 GGTACC G^G[CT][GA]CC BbsI 775 GAAGAC GAAGAC(2/6) BpuAI 775 GAAGAC GAAGAC(2/6) BsaI 1201 GAGACC GGTCTC(1/5) BsoBI 557 CTCGAG C^[CT]CG[GA]G BspMI 118 ACCTGC ACCTGC(4/8) BsrDI 1129 GCAATG GCAATG(2/0) BssSI 1277 CACGAG CACGAG(-5/-1) BstAPI 113 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 562 GGATG GGATG(2/0) BstXI 213 CCAGTTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 694 CCTCAGG CC^T[ACGT]AGG DpnII 238 GATC ^GATC DraIII 332 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 694 CCTCAGG CC^T[ACGT]AGG FokI 562 GGATG GGATG(9/13) HgaI 1093 GACGC GACGC(5/10) HindIII 143 AAGCTT A^AGCTT KpnI 231 GGTACC GGTAC^C MboI 238 GATC ^GATC MfeI 1004 CAATTG C^AATTG MunI 1004 CAATTG C^AATTG NcoI 863 CCATGG C^CATGG NdeII 238 GATC ^GATC PaeR7I 557 CTCGAG C^TCGAG SacI 1399 GAGCTC GAGCT^C SapI 1312 GAAGAGC GCTCTTC(1/4) Sau3AI 238 GATC ^GATC SfcI 961 CTACAG C^T[GA][CT]AG XhoI 557 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Alw44I 73 GTGCAC G^TGCAC 371 GTGCAC ApaLI 73 GTGCAC G^TGCAC 371 GTGCAC BpmI 211 CTCCAG CTGGAG(16/14) 968 CTCCAG Bsp1407I 1291 TGTACA T^GTACA 1389 TGTACA BsrGI 1291 TGTACA T^GTACA 1389 TGTACA BssKI 228 CCAGG ^CC[ACGT]GG 993 CCAGG BstEII 759 GGTTACC G^GT[ACGT]ACC 1024 GGTGACC BstNI 228 CCAGG CC^[AT]GG 993 CCAGG BstPI 759 GGTTACC G^GT[ACGT]ACC 1024 GGTGACC DsaI 863 CCATGG C^C[GA][CT]GG 1335 CCGTGG EcoO65I 759 GGTTACC G^GT[ACGT]ACC 1024 GGTGACC EcoRII 228 CCAGG ^CC[AT]GG 993 CCAGG EcoT14I 471 CCTTGG C^C[AT][AT]GG 863 CCATGG HincII 196 GTCAAC GT[CT]^[GA]AC 419 GTCAAC HindII 196 GTCAAC GT[CT]^[GA]AC 419 GTCAAC MvaI 228 CCAGG CC^[AT]GG 993 CCAGG NheI 355 GCTAGC G^CTAGC 1375 GCTAGC NlaIV 223 GGAGCC GG[ACGT]^[ACGT]CC 231 GGTACC PflMI 331 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 533 CCAATCTATGG PvuII 793 CAGCTG CAG^CTG 1117 CAGCTG ScrFI 228 CCAGG CC^[ACGT]GG 993 CCAGG SmlI 557 CTCGAG C^T[CT][GA]AG 642 CTCAAG SspBI 1291 TGTACA T^GTACA 1389 TGTACA StuI 596 AGGCCT AGG^CCT 1051 AGGCCT StyI 471 CCTTGG C^C[AT][AT]GG 863 CCATGG Tsp45I 403 GTGAC ^GT[GC]AC 1025 GTGAC Van91I 331 CCACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 533 CCAATCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI BanII BbvCI BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI Cac8I Cfr13I DdeI EarI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MspA1I MwoI NlaIII NspI PleI RsaI Sau96I SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I