(Circular) (Six-base) MAPSORT of: pFN21AE5653.seq Check: 8027 from: 1 to: 6223 pFN21AE5653 6223 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 2236 3544 2236 Size: 1308 4915 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2822 4286 5670 5938 1632 Size: 1190 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 1190 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2320 2550 3487 1184 Size: 1136 230 937 3920 Fragments arranged by size: 3920 1136 937 230 AlwNI CAG_nnn'CTG Cuts at: 3101 3966 5538 3101 Size: 865 1572 3786 Fragments arranged by size: 3786 1572 865 ApaLI G'TGCA_C Cuts at: 2078 2376 4280 5436 2078 Size: 298 1904 1156 2865 Fragments arranged by size: 2865 1904 1156 298 ApoI r'AATT_y Cuts at: 1493 2033 2063 2810 3532 3758 3910 6019 1493 Size: 540 30 747 722 226 152 2109 1697 Fragments arranged by size: 2109 1697 747 722 540 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2562 3548 1870 Size: 73 619 986 4545 Fragments arranged by size: 4545 986 619 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2726 2759 2726 Size: 33 6190 BamHI G'GATC_C Cuts at: 3553 3920 3553 Size: 367 5856 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3544 4999 618 Size: 325 217 627 449 1308 1455 1842 Fragments arranged by size: 1842 1455 1308 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2233 2563 3408 3542 1391 Size: 842 330 845 134 4072 Fragments arranged by size: 4072 845 842 330 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2787 928 Size: 971 888 4364 Fragments arranged by size: 4364 971 888 BbvCI CC'TCA_GC Cuts at: 2523 3213 3219 2523 Size: 690 6 5527 Fragments arranged by size: 5527 690 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4414 4448 1903 Size: 34 50 34 2393 34 3678 Fragments arranged by size: 3678 2393 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4834 136 Size: 107 122 71 631 605 3162 1525 Fragments arranged by size: 3162 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6218 6218 Size: 6223 BlpI GC'TnA_GC Cuts at: 3640 3640 Size: 6223 Bme1580I G_kGCm'C Cuts at: 1790 2082 2380 3392 4284 5440 1790 Size: 292 298 1012 892 1156 2573 Fragments arranged by size: 2573 1156 1012 892 298 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3954 4912 442 Size: 416 53 83 399 24 2537 958 1753 Fragments arranged by size: 2537 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2364 3384 1056 Size: 1308 1020 3895 Fragments arranged by size: 3895 1308 1020 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2958 4883 1239 Size: 962 757 1925 2579 Fragments arranged by size: 2579 1925 962 757 Bpu10I CC'TnA_GC Cuts at: 2523 3213 3219 2523 Size: 690 6 5527 Fragments arranged by size: 5527 690 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2632 4348 5213 5511 2632 Size: 1716 865 298 3344 Fragments arranged by size: 3344 1716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3200 4886 882 Size: 617 1701 1686 2219 Fragments arranged by size: 2219 1701 1686 617 BsaAI yAC'GTr Cuts at: 493 1204 4014 493 Size: 711 2810 2702 Fragments arranged by size: 2810 2702 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4030 4412 5047 275 Size: 53 83 186 936 2497 382 635 1451 Fragments arranged by size: 2497 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4656 5054 5328 5475 5886 4656 Size: 398 274 147 411 4993 Fragments arranged by size: 4993 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 1178 Size: 30 165 30 817 30 5151 Fragments arranged by size: 5151 817 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6223 BseYI C'CCAG_C Cuts at: 3034 5426 5922 3034 Size: 2392 496 3335 Fragments arranged by size: 3335 2392 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2696 3471 1163 Size: 573 297 663 775 3915 Fragments arranged by size: 3915 775 663 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3673 4434 5462 1540 Size: 275 186 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2380 3408 4284 4369 5440 2082 Size: 298 1028 876 85 1071 2865 Fragments arranged by size: 2865 1071 1028 876 298 85 BsmI GAATG_Cn' Cuts at: 3734 3827 3734 Size: 93 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2233 2380 2563 3392 3408 3542 Size: 399 292 151 147 183 829 16 134 Cuts at: 3542 4284 4369 5440 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 829 742 399 292 183 151 147 134 85 16 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 4112 5842 4112 Size: 1730 4493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2132 3578 844 Size: 1288 1446 3489 Fragments arranged by size: 3489 1446 1288 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3141 4718 4892 65 Size: 1183 1893 1577 174 1396 Fragments arranged by size: 1893 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4867 5054 1700 Size: 110 3057 187 2869 Fragments arranged by size: 3057 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3296 3394 96 Size: 3200 98 2925 Fragments arranged by size: 3200 2925 98 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 3282 4283 5295 3282 Size: 1001 1012 4210 Fragments arranged by size: 4210 1012 1001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2124 794 Size: 1330 4893 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 2764 3029 1184 Size: 1580 265 4378 Fragments arranged by size: 4378 1580 265 BstXI CCAn_nnnn'nTGG Cuts at: 2225 2225 Size: 6223 BstYI r'GATC_y Cuts at: 1972 3553 3920 4305 4322 5774 5860 5872 6218 Size: 1581 367 385 17 1452 86 12 346 Cuts at: 6218 1972 Size: 1977 Fragments arranged by size: 1977 1581 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2700 2700 Size: 6223 BtgI C'CryG_G Cuts at: 513 1065 2868 3340 513 Size: 552 1803 472 3396 Fragments arranged by size: 3396 1803 552 472 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 3459 3739 4533 4553 3459 Size: 280 794 20 5129 Fragments arranged by size: 5129 794 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DraIII CAC_nnn'GTG Cuts at: 2342 2342 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3670 4559 6188 Size: 54 1425 50 275 18 1840 889 1629 Cuts at: 6188 8 Size: 43 Fragments arranged by size: 1840 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 2791 3312 3484 3510 4153 1353 Size: 1239 199 521 172 26 643 3423 Fragments arranged by size: 3423 1239 643 521 199 172 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4810 5184 5330 387 Size: 1064 138 3221 374 146 1280 Fragments arranged by size: 3221 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3406 3406 Size: 6223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2822 2958 4286 4883 5670 5938 Size: 393 569 621 136 1328 597 787 268 Cuts at: 5938 1239 Size: 1524 Fragments arranged by size: 1524 1328 787 621 597 569 393 268 136 EcoRI G'AATT_C Cuts at: 3532 3910 3532 Size: 378 5845 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 6223 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 5370 1678 Size: 3692 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2768 2800 4864 Size: 32 135 32 662 32 502 32 2064 Cuts at: 4864 4896 4938 4970 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2064 662 502 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2203 2426 3567 3813 669 Size: 988 281 265 223 1141 246 3079 Fragments arranged by size: 3079 1141 988 281 265 246 223 HindIII A'AGCT_T Cuts at: 748 2148 748 Size: 1400 4823 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KpnI G_GTAC'C Cuts at: 2240 3548 2240 Size: 1308 4915 MfeI C'AATT_G Cuts at: 3009 3822 3009 Size: 813 5410 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2206 5337 5521 1305 Size: 901 3131 184 2007 Fragments arranged by size: 3131 2007 901 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4401 Fragments arranged by size: 4401 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4181 4540 4699 518 Size: 648 843 2172 359 159 2042 Fragments arranged by size: 2172 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2552 2800 3124 3639 4316 5464 5709 1606 Size: 946 248 324 515 677 1148 245 2120 Fragments arranged by size: 2120 1148 946 677 515 324 248 245 NcoI C'CATG_G Cuts at: 513 1065 2868 513 Size: 552 1803 3868 Fragments arranged by size: 3868 1803 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NheI G'CTAG_C Cuts at: 1052 2360 3380 1052 Size: 1308 1020 3895 Fragments arranged by size: 3895 1308 1020 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2947 3250 3474 3581 5126 1132 Size: 97 1718 303 224 107 1545 2229 Fragments arranged by size: 2229 1718 1545 303 224 107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6223 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 2544 1108 Size: 1234 202 4787 Fragments arranged by size: 4787 1234 202 PfoI T'CCnGG_A Cuts at: 2997 2997 Size: 6223 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PspXI vC'TCGA_Gb Cuts at: 2562 2562 Size: 6223 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2490 2522 3280 3312 2490 Size: 32 758 32 5401 Fragments arranged by size: 5401 758 32 32 PstI C_TGCA'G Cuts at: 830 3575 830 Size: 2745 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 PvuII CAG'CTG Cuts at: 2800 3124 2800 Size: 324 5899 SacI G_AGCT'C Cuts at: 3408 3408 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6223 SbfI CC_TGCA'GG Cuts at: 3575 3575 Size: 6223 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 2966 3571 4706 5387 5578 826 Size: 219 1921 605 1135 681 191 1471 Fragments arranged by size: 1921 1471 1135 681 605 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2562 2647 4327 5228 5490 820 Size: 197 926 619 85 1680 901 262 1553 Fragments arranged by size: 1680 1553 926 901 619 262 197 85 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2603 3058 1517 Size: 1086 455 4682 Fragments arranged by size: 4682 1086 455 StyI C'CwwG_G Cuts at: 513 1065 2476 2868 3662 513 Size: 552 1411 392 794 3074 Fragments arranged by size: 3074 1411 794 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4250 4267 4420 4605 5050 325 Size: 1255 2670 17 153 185 445 1498 Fragments arranged by size: 2670 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2191 3296 3394 Size: 274 80 33 51 1443 214 1105 98 Cuts at: 3394 4469 6181 96 Size: 1075 1712 138 Fragments arranged by size: 1712 1443 1105 1075 274 214 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6183 6215 6183 Size: 32 6191 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6223 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 2562 1943 Size: 619 5604 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI