################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MluI MluNI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 10 TCCGGA T^CCGGA AcsI 112 GAATTC [GA]^AATT[CT] AfaI 834 GTAC GT^AC AhdI 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 419 AGCGCT AGC^GCT ApoI 112 GAATTC [GA]^AATT[CT] Asp700I 490 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 627 GGGCTC G[GA]GC[CT]^C BbsI 406 GAAGAC GAAGAC(2/6) BcgI 438 CGAAAGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 406 GAAGAC GAAGAC(2/6) BsaWI 10 TCCGGA [AT]^CCGG[AT] BseAI 10 TCCGGA T^CCGGA BseRI 31 GAGGAG GAGGAG(10/8) BspEI 10 TCCGGA T^CCGGA BspMI 738 ACCTGC ACCTGC(4/8) BsrDI 648 CATTGC GCAATG(2/0) BsrFI 269 ACCGGC [GA]^CCGG[CT] BssHII 384 GCGCGC G^CGCGC BstXI 581 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 269 ACCGGC [GA]^CCGG[CT] DsaI 623 CCACGG C^C[GA][CT]GG Eam1105I 187 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 419 AGCGCT AGC^GCT EcoRI 112 GAATTC G^AATTC EcoRV 750 GATATC GAT^ATC EcoT14I 284 CCTTGG C^C[AT][AT]GG FauI 899 GCGGG CCCGC(4/6) HaeII 419 AGCGCT [GA]GCGC^[CT] HgaI 153 GACGC GACGC(5/10) MaeII 699 ACGT A^CGT MfeI 325 CAATTG C^AATTG MroI 10 TCCGGA T^CCGGA MseI 317 TTAA T^TAA MunI 325 CAATTG C^AATTG PflMI 865 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 834 GTAC GT^AC SexAI 985 ACCAGGT A^CC[AT]GGT SmlI 669 CTCAAG C^T[CT][GA]AG StyI 284 CCTTGG C^C[AT][AT]GG Tru9I 317 TTAA T^TAA Van91I 865 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 923 CCAGCGTGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 490 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 300 CGCG CG^CG 385 CGCG BcnI 719 CCGGG CC^[GC]GG 746 CCCGG BglII 57 AGATCT A^GATCT 275 AGATCT BmyI 627 GGGCTC G[AGT]GC[ACT]^C 794 GGGCAC BpmI 349 CTGGAG CTGGAG(16/14) 799 CTGGAG BsaI 185 GAGACC GGTCTC(1/5) 773 GAGACC BsmBI 151 GAGACG CGTCTC(1/5) 906 GAGACG BsmFI 724 GGGAC GGGAC(10/14) 843 GTCCC Bsp1286I 627 GGGCTC G[AGT]GC[ACT]^C 794 GGGCAC BsrI 537 ACTGG ACTGG(1/-1) 798 ACTGG BssSI 954 CACGAG CACGAG(-5/-1) 829 CTCGTG BstF5I 291 GGATG GGATG(2/0) 510 GGATG BstUI 300 CGCG CG^CG 385 CGCG BstYI 57 AGATCT [GA]^GATC[CT] 275 AGATCT EaeI 539 TGGCCG [CT]^GGCC[GA] 910 CGGCCA FokI 291 GGATG GGATG(9/13) 510 GGATG HphI 937 GGTGA GGTGA(8/7) 654 TCACC MboII 37 GAAGA GAAGA(8/7) 406 GAAGA MflI 57 AGATCT [GA]^GATC[CT] 275 AGATCT MvnI 300 CGCG CG^CG 385 CGCG NciI 719 CCGGG CC^[GC]GG 746 CCCGG PvuII 157 CAGCTG CAG^CTG 611 CAGCTG TaqI 404 TCGA T^CGA 437 TCGA TspRI 587 CAGTG CA[GC]TG[ACGT][ACGT]^ 797 CACTG TthHB8I 404 TCGA T^CGA 437 TCGA XhoII 57 AGATCT [GA]^GATC[CT] 275 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BsaJI BsgI BsiYI BslI BsmAI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI TfiI TseI Tsp509I